FastQCFastQC Report
Thu 26 May 2016
SRR1547918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547918_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2498761
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432631.731378070971974No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35750.14307090594098434No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC25570.10233071510240474No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC207.034878E-445.00000435
CGTTTTT222700.043.2521321
TCACGAC6800.043.01470625
CGGTCTA6650.042.63157731
CGACGGT6950.040.79136328
CACGACG7300.039.76027326
CTCACGA7450.039.26174524
ACGGTCT7250.039.10344730
TACGGGA14400.039.0625044
GGGCGAT36450.038.950627
ATCTCGT700.038.5714319
TAGGGCG8500.038.3823555
TAAGGGA38100.038.2086644
GGCGATA8600.038.1976748
AGGGCGA19200.038.0859346
TAGTACG1500.037.51
CGTTGCG301.14030554E-437.49999611
CGGTTTT17000.037.3235321
ACGGGAT14900.037.2986565
TCTCACG7800.037.21153623