##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547918_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2498761 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34835984714024 31.0 31.0 33.0 30.0 34.0 2 31.70779518329284 31.0 31.0 34.0 30.0 34.0 3 31.546471231142153 31.0 31.0 34.0 30.0 34.0 4 35.49338972394719 37.0 35.0 37.0 33.0 37.0 5 35.511162131952595 37.0 35.0 37.0 33.0 37.0 6 35.607776814189116 37.0 35.0 37.0 33.0 37.0 7 35.90147036871473 37.0 35.0 37.0 35.0 37.0 8 35.9074129138401 37.0 35.0 37.0 35.0 37.0 9 37.67080044870238 39.0 37.0 39.0 35.0 39.0 10 37.204109956894634 39.0 37.0 39.0 33.0 39.0 11 37.065459641798476 39.0 37.0 39.0 33.0 39.0 12 36.723863946972116 39.0 35.0 39.0 32.0 39.0 13 36.63441721717283 39.0 35.0 39.0 32.0 39.0 14 37.75500738165835 40.0 36.0 41.0 33.0 41.0 15 37.8661312546498 40.0 36.0 41.0 33.0 41.0 16 37.89484508522424 40.0 36.0 41.0 33.0 41.0 17 37.805080197746 40.0 36.0 41.0 33.0 41.0 18 37.622203163888024 39.0 36.0 41.0 33.0 41.0 19 37.45414827588553 39.0 36.0 41.0 32.0 41.0 20 37.200473354594536 39.0 35.0 41.0 32.0 41.0 21 37.096227690443385 39.0 35.0 41.0 32.0 41.0 22 37.03799042805615 39.0 35.0 41.0 32.0 41.0 23 36.901401534600545 39.0 35.0 41.0 32.0 41.0 24 36.8521815411718 38.0 35.0 41.0 31.0 41.0 25 36.858961701419226 39.0 35.0 41.0 31.0 41.0 26 36.76442925113686 39.0 35.0 41.0 31.0 41.0 27 36.702818316757785 38.0 35.0 41.0 31.0 41.0 28 36.62328930217816 38.0 35.0 41.0 31.0 41.0 29 36.58115922251068 38.0 35.0 41.0 31.0 41.0 30 36.467464475394 38.0 35.0 41.0 31.0 41.0 31 36.276921642365956 38.0 35.0 41.0 30.0 41.0 32 35.98485529428385 38.0 35.0 41.0 29.0 41.0 33 35.67378432751271 38.0 35.0 41.0 27.0 41.0 34 35.324113430616215 38.0 34.0 41.0 25.0 41.0 35 35.09941687100127 38.0 34.0 41.0 24.0 41.0 36 34.958588676548096 38.0 34.0 41.0 23.0 41.0 37 34.842291439637485 38.0 34.0 40.0 23.0 41.0 38 34.68200400118299 38.0 34.0 40.0 22.0 41.0 39 34.58920681089548 38.0 34.0 40.0 21.0 41.0 40 34.43002512044969 38.0 34.0 40.0 21.0 41.0 41 34.29942039274665 38.0 33.0 40.0 20.0 41.0 42 34.2730409190795 38.0 33.0 40.0 20.0 41.0 43 34.21193183341664 38.0 33.0 40.0 19.0 41.0 44 34.0857601027069 38.0 33.0 40.0 18.0 41.0 45 33.98997623222069 38.0 33.0 40.0 19.0 41.0 46 33.94112081947814 37.0 33.0 40.0 19.0 41.0 47 33.896546728558675 37.0 33.0 40.0 19.0 41.0 48 33.757705919053485 37.0 33.0 40.0 18.0 41.0 49 33.62186819787887 37.0 33.0 40.0 18.0 41.0 50 33.54387754571165 37.0 33.0 40.0 18.0 41.0 51 33.272368986069495 36.0 32.0 40.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 2.0 12 6.0 13 16.0 14 38.0 15 79.0 16 211.0 17 549.0 18 1286.0 19 2573.0 20 4357.0 21 6833.0 22 10585.0 23 16019.0 24 25299.0 25 39726.0 26 54568.0 27 60554.0 28 59062.0 29 58484.0 30 62344.0 31 71195.0 32 84724.0 33 106856.0 34 160441.0 35 213556.0 36 184873.0 37 241464.0 38 381070.0 39 651896.0 40 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.770074048698536 23.407120568953975 27.60796250621808 19.21484287612941 2 35.389098837383806 24.67454870633886 27.476697451256843 12.459655005020489 3 27.062772309956813 24.029749143675605 35.80406449436341 13.103414052004172 4 24.12767767705675 25.834163411386683 36.20770453836922 13.83045437318735 5 22.092509047483933 30.294974189208173 34.10790387716152 13.504612886146376 6 20.45829913305034 38.68381169707707 30.840164385469436 10.017724784403152 7 83.5487267489768 2.6796080137316056 12.253352761628664 1.5183124756629385 8 83.92555350431674 2.399989434763869 11.980857713082605 1.693599347836788 9 78.3202154988012 5.57288192027969 13.281422272878437 2.8254803080406647 10 28.38462742135002 41.473194115003395 18.839176695970522 11.30300176767606 11 28.956710945944806 22.007466900595933 32.433714148732115 16.602108004727143 12 28.295743370414378 20.52829382241839 33.0329311206634 18.14303168650383 13 24.115951865744663 23.463108316481648 34.10702344081727 18.313916376956417 14 18.74240873777044 27.329264383428427 34.74037733100525 19.18794954779589 15 17.989195445262673 25.560667866994883 38.84941376946415 17.6007229182783 16 22.756598170053078 24.14352553125329 36.29766912481826 16.802207173875374 17 21.555522917157745 23.510691898905097 33.78506387765777 21.14872130627939 18 21.587058546215506 24.136121861994805 35.366487631270054 18.910331960519635 19 23.115456020003514 25.27580668979546 32.4374760131121 19.171261277088924 20 23.857943997044938 25.936454106655262 32.3998173494784 17.805784546821403 21 23.44397883591108 24.63737028071112 34.83586465452278 17.08278622885502 22 22.423433053421277 21.852790242844353 34.40869294822514 21.31508375550923 23 19.113312557703598 25.85729487534022 34.66790141193975 20.361491155016427 24 19.32545769683455 25.681967983332537 36.08068158579392 18.91189273403899 25 20.816116467321205 26.27878376523405 33.64859624429867 19.25650352314607 26 19.766276166468103 27.45768803018776 32.117637501145566 20.65839830219857 27 18.97732516235046 26.488047476329267 34.52002812593922 20.014599235381056 28 17.78153252752064 26.195822649705192 36.16040109478257 19.862243727991594 29 19.47369116133956 24.591667630477666 35.35328108610628 20.5813601220765 30 19.94272361382301 24.74718470473967 35.47802290815328 19.83206877328404 31 21.971409030315424 25.071865616599588 33.3205936862309 19.63613166685409 32 22.725102560829146 25.06210077714515 33.20889833001235 19.003898332013346 33 22.40278281916518 25.314025631102776 32.200158398502296 20.08303315122975 34 19.598392963552737 26.763303893409574 33.528776861812716 20.109526281224976 35 20.061822639300036 27.040241143510723 32.23441537626047 20.663520840928765 36 22.698969609338388 27.127164222588718 30.7349522423313 19.438913925741595 37 20.890833497081154 28.702504961458896 30.846007281208564 19.560654260251383 38 21.04186834995424 29.08117262915501 29.900018449143396 19.97694057174736 39 21.114864526859513 28.003718642959452 29.826461994564507 21.05495483561653 40 22.002864619705527 27.00506370957447 30.721585617832197 20.27048605288781 41 20.089276245307175 27.070416098218274 30.687248600406363 22.153059056068187 42 21.13683541563199 27.4203495252247 29.956606494178516 21.486208564964794 43 21.020417719021548 26.951717271079545 30.39858553899312 21.629279470905782 44 20.948382018128182 27.465371838283055 30.33087198015336 21.2553741634354 45 19.844875120109524 28.46874911205994 29.649174130699173 22.03720163713136 46 20.82756213979648 28.163517839441226 30.135134972892562 20.873785047869724 47 20.57799845603481 27.43979916446591 30.947137401296082 21.035064978203195 48 20.472426134392204 27.258349237882296 31.48460376962823 20.784620858097274 49 21.074964752531354 26.28830848568551 31.107056657279347 21.529670104503793 50 19.951207818594895 27.799217292089963 31.39291833032451 20.856656558990636 51 19.60375562128591 27.778006780160247 30.87938382262249 21.738853775931354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1705.0 1 2868.0 2 4031.0 3 36253.5 4 68476.0 5 48899.0 6 29322.0 7 26807.0 8 24292.0 9 24676.5 10 25061.0 11 24878.0 12 24695.0 13 24008.0 14 23321.0 15 22163.0 16 21005.0 17 19795.0 18 18585.0 19 17843.5 20 17102.0 21 16575.0 22 16048.0 23 17163.0 24 18278.0 25 20206.5 26 25431.0 27 28727.0 28 34355.5 29 39984.0 30 43155.0 31 46326.0 32 54786.0 33 63246.0 34 70027.5 35 76809.0 36 83653.5 37 90498.0 38 97166.0 39 103834.0 40 114394.0 41 124954.0 42 138729.5 43 152505.0 44 160313.0 45 168121.0 46 179799.5 47 191478.0 48 191917.0 49 192356.0 50 185516.5 51 178677.0 52 164686.0 53 150695.0 54 140149.0 55 129603.0 56 121500.0 57 113397.0 58 104784.0 59 96171.0 60 85820.5 61 75470.0 62 66117.0 63 56764.0 64 47905.0 65 39046.0 66 31785.0 67 24524.0 68 20068.5 69 15613.0 70 13216.0 71 10819.0 72 8673.0 73 6527.0 74 5373.5 75 3135.5 76 2051.0 77 1613.0 78 1175.0 79 848.5 80 522.0 81 429.5 82 337.0 83 232.0 84 127.0 85 86.5 86 46.0 87 32.5 88 19.0 89 15.5 90 12.0 91 21.0 92 30.0 93 23.5 94 17.0 95 9.5 96 2.0 97 2.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2498761.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.111243611383614 #Duplication Level Percentage of deduplicated Percentage of total 1 74.85475916572345 18.796960928819324 2 6.979876880109449 3.5054677742778524 3 3.0702702172472676 2.3129491013421553 4 1.7999788346165093 1.8079882804555816 5 1.2310180639648942 1.545619724711814 6 0.9541823879049899 1.4376423835424363 7 0.7956578866586218 1.3985971316242318 8 0.6517270341063088 1.3092541057254432 9 0.5447886517598424 1.2312288495952772 >10 8.306647983078161 50.04388000478911 >50 0.7592456464295433 11.685312752940007 >100 0.046971932719678255 1.9600412721226173 >500 0.003250210454202045 0.5690325143840289 >1k 0.0014625947043909202 0.630530793154592 >5k 0.0 0.0 >10k+ 1.6251052271010224E-4 1.7654943825155078 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43263 1.731378070971974 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3575 0.14307090594098434 No Hit GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC 2557 0.10233071510240474 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.037378524796889336 0.0 2 0.0 0.0 0.0 0.11693795445022553 0.0 3 0.0 0.0 0.0 0.20226024017503075 0.0 4 0.0 0.0 0.0 0.33120414477415006 0.0 5 0.0 0.0 0.0 0.6824582262969527 0.0 6 0.0 0.0 0.0 1.1349624874087598 0.0 7 0.0 0.0 0.0 1.4393933633508766 0.0 8 0.0 0.0 0.0 1.9412020597408075 0.0 9 0.0 0.0 0.0 2.2458330348520725 0.0 10 4.001983382964597E-5 0.0 0.0 2.700458347156851 0.0 11 4.001983382964597E-5 0.0 0.0 3.008170849472999 0.0 12 4.001983382964597E-5 0.0 0.0 3.300275616595585 0.0 13 4.001983382964597E-5 0.0 0.0 3.441185451509768 0.0 14 4.001983382964597E-5 0.0 0.0 3.5277483520832926 0.0 15 4.001983382964597E-5 0.0 0.0 3.6076279404072658 0.0 16 4.001983382964597E-5 0.0 0.0 3.7270471245549293 0.0 17 4.001983382964597E-5 0.0 0.0 3.864475233925934 0.0 18 4.001983382964597E-5 0.0 0.0 4.037120797067026 0.0 19 4.001983382964597E-5 0.0 0.0 4.1478956971074865 0.0 20 4.001983382964597E-5 0.0 0.0 4.273277836495767 0.0 21 4.001983382964597E-5 0.0 0.0 4.417389258116322 0.0 22 4.001983382964597E-5 0.0 0.0 4.586913274218703 0.0 23 4.001983382964597E-5 0.0 0.0 4.765401733098924 0.0 24 4.001983382964597E-5 0.0 0.0 4.900548711941639 0.0 25 4.001983382964597E-5 0.0 0.0 5.029932834712883 0.0 26 4.001983382964597E-5 0.0 0.0 5.153474061745001 0.0 27 4.001983382964597E-5 0.0 0.0 5.287220346403678 0.0 28 4.001983382964597E-5 0.0 0.0 5.429931073840195 0.0 29 4.001983382964597E-5 0.0 0.0 5.590810805835372 0.0 30 4.001983382964597E-5 0.0 0.0 5.792790907173595 0.0 31 4.001983382964597E-5 0.0 0.0 5.962234883608316 0.0 32 4.001983382964597E-5 0.0 0.0 6.130078066689851 0.0 33 4.001983382964597E-5 0.0 0.0 6.29988222162904 0.0 34 4.001983382964597E-5 0.0 0.0 6.479331156521172 0.0 35 4.001983382964597E-5 0.0 0.0 6.677549393479408 0.0 36 4.001983382964597E-5 0.0 0.0 6.865642612478744 0.0 37 4.001983382964597E-5 0.0 0.0 7.056217061175518 0.0 38 4.001983382964597E-5 0.0 0.0 7.271923965517311 0.0 39 4.001983382964597E-5 0.0 0.0 7.593523350172346 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTATC 20 7.034878E-4 45.000004 35 CGTTTTT 22270 0.0 43.252132 1 TCACGAC 680 0.0 43.014706 25 CGGTCTA 665 0.0 42.631577 31 CGACGGT 695 0.0 40.791363 28 CACGACG 730 0.0 39.760273 26 CTCACGA 745 0.0 39.261745 24 ACGGTCT 725 0.0 39.103447 30 TACGGGA 1440 0.0 39.062504 4 GGGCGAT 3645 0.0 38.95062 7 ATCTCGT 70 0.0 38.57143 19 TAGGGCG 850 0.0 38.382355 5 TAAGGGA 3810 0.0 38.208664 4 GGCGATA 860 0.0 38.197674 8 AGGGCGA 1920 0.0 38.085934 6 TAGTACG 150 0.0 37.5 1 CGTTGCG 30 1.14030554E-4 37.499996 11 CGGTTTT 1700 0.0 37.323532 1 ACGGGAT 1490 0.0 37.298656 5 TCTCACG 780 0.0 37.211536 23 >>END_MODULE