FastQCFastQC Report
Thu 26 May 2016
SRR1547917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547917_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2166807
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457632.112001668814989No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT38710.17864996744057038No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37020.17085047260785108No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC29470.13600657557410512No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT27670.1276994213144041No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24930.1150540864968592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG600.045.0000041
CGACTCA253.8919527E-545.00000442
CTACGCG351.2125929E-745.01
CGTTTTT178700.043.9045871
CGACGGT4500.042.028
AGGGCGA16850.041.528196
TCGCACG556.184564E-1140.9090921
CGATCGT501.0822987E-940.50000410
CGGTTTT13750.040.2545431
CGGTCTA4700.040.21276531
GACCGTT451.929584E-840.09
GGGCGAT32350.039.853177
CACGACG4750.039.78947426
TATAGCG1200.039.3750041
ATGACGG1550.039.1935462
GGCGATA7350.039.1836748
TCACGAC5000.039.14999825
TACGGGA10550.039.028444
TAGGGAC28100.038.9946635
CGTAAGG2950.038.8983042