##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547917_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2166807
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334410494335675	31.0	31.0	33.0	30.0	34.0
2	31.69572555377567	31.0	31.0	34.0	30.0	34.0
3	31.521708209360593	31.0	31.0	34.0	30.0	34.0
4	35.45401736287542	37.0	35.0	37.0	33.0	37.0
5	35.46399240910704	37.0	35.0	37.0	33.0	37.0
6	35.55966821225887	37.0	35.0	37.0	33.0	37.0
7	35.937057153682815	37.0	35.0	37.0	35.0	37.0
8	35.962123530152894	37.0	35.0	37.0	35.0	37.0
9	37.729253228367824	39.0	38.0	39.0	35.0	39.0
10	37.21220533254692	39.0	37.0	39.0	33.0	39.0
11	36.93648211400462	39.0	37.0	39.0	33.0	39.0
12	36.614556349504134	39.0	35.0	39.0	32.0	39.0
13	36.52258553715213	39.0	35.0	39.0	32.0	39.0
14	37.65069108600812	40.0	36.0	41.0	32.0	41.0
15	37.795067581007444	40.0	36.0	41.0	33.0	41.0
16	37.84996494842411	40.0	36.0	41.0	33.0	41.0
17	37.781913202237206	40.0	36.0	41.0	33.0	41.0
18	37.60678177613419	39.0	36.0	41.0	33.0	41.0
19	37.459934364251176	39.0	36.0	41.0	32.0	41.0
20	37.23253201600327	39.0	35.0	41.0	32.0	41.0
21	37.12668641000329	39.0	35.0	41.0	32.0	41.0
22	37.05024351499695	39.0	35.0	41.0	32.0	41.0
23	36.90302643474938	38.0	35.0	41.0	32.0	41.0
24	36.868518977463154	38.0	35.0	41.0	32.0	41.0
25	36.858701767162465	38.0	35.0	41.0	32.0	41.0
26	36.757773996484225	38.0	35.0	41.0	31.0	41.0
27	36.67951414223786	38.0	35.0	41.0	31.0	41.0
28	36.60208731096032	38.0	35.0	41.0	31.0	41.0
29	36.573183952239404	38.0	35.0	41.0	31.0	41.0
30	36.46102121693349	38.0	35.0	40.0	31.0	41.0
31	36.236888195395345	38.0	35.0	40.0	30.0	41.0
32	35.90954108972326	38.0	35.0	40.0	30.0	41.0
33	35.64772173986885	38.0	35.0	40.0	28.0	41.0
34	35.346390795303876	38.0	34.0	40.0	25.0	41.0
35	35.16008393917871	38.0	34.0	40.0	25.0	41.0
36	35.02668119495645	38.0	34.0	40.0	24.0	41.0
37	34.93406888569217	38.0	34.0	40.0	24.0	41.0
38	34.82283009054337	38.0	34.0	40.0	23.0	41.0
39	34.763930059299234	38.0	34.0	40.0	23.0	41.0
40	34.64865860226592	38.0	34.0	40.0	23.0	41.0
41	34.52396175570782	38.0	34.0	40.0	22.0	41.0
42	34.492850078479535	38.0	34.0	40.0	22.0	41.0
43	34.410832621456365	38.0	34.0	40.0	22.0	41.0
44	34.270521093941454	37.0	33.0	40.0	21.0	41.0
45	34.16809849700504	37.0	33.0	40.0	21.0	41.0
46	34.10657986613482	37.0	33.0	40.0	21.0	41.0
47	34.03282110497151	37.0	33.0	40.0	20.0	41.0
48	33.85873038069381	37.0	33.0	40.0	20.0	41.0
49	33.71790657866621	37.0	33.0	40.0	20.0	41.0
50	33.62193587153816	36.0	33.0	40.0	19.0	41.0
51	33.33725246410963	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	4.0
13	6.0
14	22.0
15	55.0
16	153.0
17	355.0
18	834.0
19	1791.0
20	3112.0
21	5261.0
22	8259.0
23	13044.0
24	20630.0
25	32776.0
26	44031.0
27	47421.0
28	47073.0
29	48054.0
30	53840.0
31	62672.0
32	76319.0
33	95884.0
34	147692.0
35	213602.0
36	172517.0
37	209413.0
38	312887.0
39	549011.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.83999359426105	24.162050427195407	28.707032975248836	18.290923003294708
2	36.15158156679391	23.560520157079058	27.896531624644005	12.391366651483034
3	27.38282643539549	23.65517556478265	36.23193020882801	12.730067790993846
4	23.81854959855677	26.56406408138796	36.01183677180293	13.605549548252336
5	21.781773826649072	30.500270674776296	34.18472434323869	13.53323115533594
6	20.185831040789513	38.9857979967759	30.5335454426721	10.294825519762488
7	83.75559059943963	2.726546480604872	11.744746994079307	1.7731159258761855
8	84.11616724516766	2.1429688938608744	11.483394690897713	2.2574691700737537
9	79.03361951479758	5.14531289588782	13.142979508557984	2.6780880807566154
10	34.942890622007404	37.43692908505464	17.818153624203724	9.802026668734225
11	26.170489572906124	23.99498432486142	34.0789004281415	15.755625674090956
12	26.056312352692235	22.326630844371465	34.019688878612634	17.59736792432367
13	21.173182475411977	27.45334494488895	34.98285726416797	16.3906153155311
14	16.245332417700332	31.50022129335931	34.63907953038734	17.615366758553023
15	15.014535212411628	27.62516458549377	41.270219267336685	16.090080934757918
16	18.591688138352886	26.245530866385426	39.5193480545337	15.643432940727992
17	18.517708314584546	25.698412456670116	33.972061194190346	21.811818034554992
18	18.75358534470306	27.37262709599886	35.673504839148116	18.20028272014997
19	21.069019991166726	28.910419802040515	32.32973679704745	17.690823409745306
20	22.2950636581846	28.868837879885014	31.376121638890776	17.459976823039618
21	20.310530656399024	28.838424465123104	33.84085430774407	17.0101905707338
22	19.714492338265472	25.748947645083298	33.435926688440645	21.100633328210588
23	16.48462461123672	30.101573421167643	33.1562986458877	20.257503321707933
24	17.316401506917785	28.39740687564698	36.93669994604965	17.349491671385593
25	18.082598034804207	29.50959637844995	33.90080427098491	18.50700131576093
26	17.52048059656444	32.27537108750341	31.290050290588873	18.914098025343282
27	17.537464111939826	31.244176338732522	33.60017758849773	17.618181960829922
28	16.476640512976008	29.78580925758501	36.17927208099291	17.558278148446078
29	17.44710073393708	27.584459529621235	35.28449926550911	19.683940470932576
30	19.786718429467875	28.85319273936257	33.965461621639584	17.39462720952997
31	22.153334376342702	28.284983387999024	31.48540686826284	18.076275367395432
32	22.54986253967243	28.993814400636513	31.557217601752257	16.899105457938802
33	21.45904088365969	29.398280511369958	31.249668290715327	17.89301031425503
34	18.17314601623495	29.561100734860098	33.384745388029486	18.881007860875474
35	18.029247644114125	29.82637586088655	33.085410929538256	19.058965565461065
36	21.296682168739533	29.331546372150356	31.429656633008847	17.942114826101264
37	17.768726056358503	32.18076183065681	32.48734197369678	17.5631701392879
38	17.6909618623163	32.253910938999184	30.603971650451562	19.451155548232954
39	17.4409165190993	31.783679857043108	31.09501676891389	19.680386854943702
40	19.86605175264802	29.43907786895649	31.040466455941857	19.65440392245364
41	17.60710575515032	28.937879561954528	31.511666705894896	21.943347977000258
42	19.51129011490179	30.349772730104714	29.70412224069795	20.43481491429555
43	19.384882917583337	29.87940319557764	30.646661193175028	20.089052693663994
44	19.01332236788971	31.443317286680355	30.30339111882138	19.23996922660855
45	18.11305760042311	32.451575059523066	29.335146138996226	20.100221201057593
46	20.129896202107524	30.655291403433715	29.741689038294595	19.473123356164162
47	19.484430316128755	30.06654492070591	30.340450256991048	20.108574506174293
48	19.19358761532522	29.398188209655956	31.164289205268396	20.24393496975042
49	19.390421020423137	28.338149175261112	31.163596942413424	21.107832861902327
50	18.12118015125482	30.304867946245327	31.383644228581502	20.19030767391835
51	17.40376507921564	30.955271973922923	30.372340499176897	21.268622447684542
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1642.0
1	2298.5
2	2955.0
3	32059.0
4	61163.0
5	41664.0
6	22165.0
7	20662.5
8	19160.0
9	21098.5
10	23037.0
11	24552.0
12	26067.0
13	26825.5
14	27584.0
15	26594.5
16	25605.0
17	24162.0
18	22719.0
19	21161.5
20	19604.0
21	19431.5
22	19259.0
23	19816.0
24	20373.0
25	22117.5
26	26298.5
27	28735.0
28	33224.5
29	37714.0
30	42422.5
31	47131.0
32	54055.0
33	60979.0
34	68651.5
35	76324.0
36	80258.5
37	84193.0
38	93079.0
39	101965.0
40	118669.5
41	135374.0
42	150526.5
43	165679.0
44	183851.5
45	202024.0
46	202747.0
47	203470.0
48	191259.0
49	179048.0
50	159541.0
51	140034.0
52	122229.5
53	104425.0
54	91863.5
55	79302.0
56	71224.5
57	63147.0
58	55515.5
59	47884.0
60	41377.0
61	34870.0
62	28773.0
63	22676.0
64	19072.5
65	15469.0
66	11930.0
67	8391.0
68	6661.0
69	4931.0
70	4051.0
71	3171.0
72	2620.0
73	2069.0
74	1552.0
75	852.0
76	669.0
77	541.0
78	413.0
79	304.5
80	196.0
81	168.0
82	140.0
83	115.0
84	90.0
85	57.5
86	25.0
87	21.0
88	17.0
89	13.5
90	10.0
91	9.0
92	8.0
93	6.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2166807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.41793383240857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74421522826155	20.493319474837804
2	8.613697251250679	4.723395625743814
3	3.6985313437851226	3.0421826298296892
4	2.111886025208092	2.316142052029752
5	1.3975972990956596	1.9159615135478862
6	0.979181475624895	1.6108279745162133
7	0.7411904349632881	1.4225337212126292
8	0.5818830470583418	1.276322470595672
9	0.4768838399630107	1.1767652612865669
>10	5.51124548910509	34.28246373567956
>50	0.9969410760770051	18.79780261193773
>100	0.14247175889111638	5.212767351110603
>500	0.0022233799724221746	0.4354454333957383
>1k	0.0018813215151264556	1.148120395354432
>5k	0.0	0.0
>10k+	1.7102922864785958E-4	2.145949748921944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45763	2.112001668814989	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	3871	0.17864996744057038	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3702	0.17085047260785108	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	2947	0.13600657557410512	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	2767	0.1276994213144041	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2493	0.1150540864968592	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.230171399667806E-5	0.0	0.0	0.1005165665423824	0.0
2	9.230171399667806E-5	0.0	0.0	0.2953654847893698	0.0
3	9.230171399667806E-5	0.0	0.0	0.49759854015609145	0.0
4	9.230171399667806E-5	0.0	0.0	0.707446486927539	0.0
5	9.230171399667806E-5	0.0	0.0	1.2979928530782852	0.0
6	9.230171399667806E-5	0.0	0.0	2.32231112415642	0.0
7	9.230171399667806E-5	0.0	0.0	3.035203412209763	0.0
8	9.230171399667806E-5	0.0	0.0	4.239971534151404	0.0
9	9.230171399667806E-5	0.0	0.0	4.945341232514017	0.0
10	9.230171399667806E-5	0.0	0.0	5.709276368407523	0.0
11	9.230171399667806E-5	0.0	0.0	6.395678064543819	0.0
12	9.230171399667806E-5	0.0	0.0	6.932689436576492	0.0
13	9.230171399667806E-5	0.0	0.0	7.207471639144603	0.0
14	9.230171399667806E-5	0.0	0.0	7.365169117507928	0.0
15	9.230171399667806E-5	0.0	0.0	7.503852442787936	0.0
16	9.230171399667806E-5	0.0	0.0	7.741898563185369	0.0
17	9.230171399667806E-5	0.0	0.0	8.031818246848935	0.0
18	9.230171399667806E-5	0.0	0.0	8.389302785158069	0.0
19	9.230171399667806E-5	0.0	0.0	8.616088096447907	0.0
20	9.230171399667806E-5	0.0	0.0	8.842919558594744	0.0
21	9.230171399667806E-5	0.0	0.0	9.095641651517647	0.0
22	9.230171399667806E-5	0.0	0.0	9.385561335181213	0.0
23	9.230171399667806E-5	0.0	0.0	9.68964933194327	0.0
24	9.230171399667806E-5	0.0	0.0	9.932310538040536	0.0
25	9.230171399667806E-5	0.0	0.0	10.134405140836263	0.0
26	9.230171399667806E-5	0.0	0.0	10.33285382592912	0.0
27	9.230171399667806E-5	0.0	0.0	10.54237871670158	0.0
28	9.230171399667806E-5	0.0	0.0	10.763856679436609	0.0
29	9.230171399667806E-5	0.0	0.0	11.005733320964904	0.0
30	9.230171399667806E-5	0.0	0.0	11.317205454846693	0.0
31	9.230171399667806E-5	0.0	0.0	11.57320425861648	0.0
32	9.230171399667806E-5	0.0	0.0	11.82491103268542	0.0
33	9.230171399667806E-5	0.0	0.0	12.070756647915573	0.0
34	9.230171399667806E-5	0.0	0.0	12.323940249408462	0.0
35	9.230171399667806E-5	0.0	0.0	12.599368563974549	0.0
36	9.230171399667806E-5	0.0	0.0	12.85744415630926	0.0
37	9.230171399667806E-5	0.0	0.0	13.121519360053757	0.0
38	9.230171399667806E-5	0.0	0.0	13.40230117403165	0.0
39	9.230171399667806E-5	0.0	0.0	13.766662190033538	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	60	0.0	45.000004	1
CGACTCA	25	3.8919527E-5	45.000004	42
CTACGCG	35	1.2125929E-7	45.0	1
CGTTTTT	17870	0.0	43.904587	1
CGACGGT	450	0.0	42.0	28
AGGGCGA	1685	0.0	41.52819	6
TCGCACG	55	6.184564E-11	40.909092	1
CGATCGT	50	1.0822987E-9	40.500004	10
CGGTTTT	1375	0.0	40.254543	1
CGGTCTA	470	0.0	40.212765	31
GACCGTT	45	1.929584E-8	40.0	9
GGGCGAT	3235	0.0	39.85317	7
CACGACG	475	0.0	39.789474	26
TATAGCG	120	0.0	39.375004	1
ATGACGG	155	0.0	39.193546	2
GGCGATA	735	0.0	39.183674	8
TCACGAC	500	0.0	39.149998	25
TACGGGA	1055	0.0	39.02844	4
TAGGGAC	2810	0.0	38.994663	5
CGTAAGG	295	0.0	38.898304	2
>>END_MODULE