FastQCFastQC Report
Thu 26 May 2016
SRR1547916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547916_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2398683
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183530.7651281974316739No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28730.11977405934840077No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100850.042.3227581
CGCGTCA603.6379788E-1241.24999639
CGACGGT3700.040.13513628
AATCGCG403.460318E-739.3751
CGGGATC6650.039.2481236
TATAGCG1900.039.078951
AGGGACC30950.039.0387736
CGGTCTA3750.039.031
TACGGGA11950.038.974894
ACGGGAT16100.038.850935
CGGGATA9900.038.4090966
GACCGAT9900.038.181829
TAAGGGA48250.038.0984464
TATGGGA35800.037.70954
CGCGGAC301.1402921E-437.49999624
CTCGACA301.1402921E-437.49999640
TACCGAT301.1402921E-437.49999636
TTAGGGA40750.037.4355854
TAGGGAT45600.037.4013185
TGCGTAG1750.037.2857131
Produced by FastQC (version 0.11.5)