FastQCFastQC Report
Thu 26 May 2016
SRR1547915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547915_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1546791
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT544823.5222599562578267No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51220.33113717367116824No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT38690.25013075457511713No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC28090.18160178071892066No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT27740.17933903158215944No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTG17820.11520625604881332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTG551.8189894E-1245.0000041
TAACGCG207.034045E-445.01
CGTTTTT146300.043.5851021
CGGTCTA2050.041.70731731
GCGATAC556.184564E-1140.9090929
TGCTACG556.184564E-1140.9090921
TAATGCG556.184564E-1140.9090921
GCGATCG950.040.2631579
CGGTTTT13800.039.9456521
GTAGGGT4250.039.705884
TCGATAG800.039.3751
TACGGGA4500.039.0000044
TAGGGAC15950.038.7931025
GACCGAT7950.038.7735869
ATTGGGA12450.038.6746984
ATTCGGC356.249691E-638.57142617
TACGTAG356.249691E-638.5714261
AGGGCGA10900.038.3944976
CACGACG2350.038.2978726
CCGCTCG1000.038.2519
Produced by FastQC (version 0.11.5)