FastQCFastQC Report
Thu 26 May 2016
SRR1547914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547914_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1732551
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59270.3420967117273893No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47330.2731809914975086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG700.045.0000041
CGTTCAA253.891628E-545.09
TCGACGA253.891628E-545.034
ATACTCG1350.043.33333644
GCGATAC800.042.18759
TAATGCG700.041.7857171
CGGGACC5550.041.7567566
CGTTTTT35800.041.1662031
TACGGGA5750.040.6956524
CGACGGT3050.040.5737728
CGTAAGG2000.040.52
TAACGCC1900.040.2631612
GATACGT451.9292202E-840.011
ATACGTA451.9292202E-840.012
TCACGAC3150.039.99999625
TAAGGGA18650.039.5710454
CGCATCG1650.039.54545221
CGGTCTA3000.039.00000431
AAATGCG750.039.0000041
ACGTAGG2950.038.8983042
Produced by FastQC (version 0.11.5)