##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547914_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1732551
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503229053574756	31.0	31.0	33.0	30.0	34.0
2	31.92317397871693	31.0	31.0	34.0	30.0	34.0
3	32.00846151137831	33.0	31.0	34.0	30.0	34.0
4	35.76660311875379	37.0	35.0	37.0	35.0	37.0
5	35.67906861039011	37.0	35.0	37.0	33.0	37.0
6	35.72168496050044	37.0	35.0	37.0	33.0	37.0
7	36.012660521970204	37.0	35.0	37.0	35.0	37.0
8	36.03613226969942	37.0	35.0	37.0	35.0	37.0
9	37.802842744600305	39.0	38.0	39.0	35.0	39.0
10	37.35605935986877	39.0	37.0	39.0	34.0	39.0
11	37.078943130678404	39.0	37.0	39.0	33.0	39.0
12	36.30501612939533	38.0	35.0	39.0	33.0	39.0
13	35.99396092813429	38.0	35.0	39.0	32.0	39.0
14	37.01202850594297	39.0	35.0	41.0	32.0	41.0
15	37.236911929288084	39.0	35.0	41.0	32.0	41.0
16	37.38438753029492	39.0	35.0	41.0	33.0	41.0
17	37.31295471244425	39.0	35.0	41.0	33.0	41.0
18	37.23701697670083	39.0	35.0	41.0	32.0	41.0
19	37.1531527787638	38.0	35.0	41.0	32.0	41.0
20	37.018588197403716	38.0	35.0	41.0	32.0	41.0
21	36.894801942338205	38.0	35.0	41.0	32.0	41.0
22	36.79172330280609	38.0	35.0	41.0	32.0	41.0
23	36.71308723379572	38.0	35.0	41.0	32.0	41.0
24	36.72123475730296	38.0	35.0	41.0	32.0	41.0
25	36.704385614045414	38.0	35.0	41.0	32.0	41.0
26	36.581105548985285	38.0	35.0	41.0	31.0	41.0
27	36.47735218184054	38.0	35.0	40.0	31.0	41.0
28	36.445316761238196	38.0	35.0	40.0	31.0	41.0
29	36.45066609871802	38.0	35.0	40.0	31.0	41.0
30	36.36002287955737	38.0	35.0	40.0	31.0	41.0
31	36.16836272063564	38.0	35.0	40.0	30.0	41.0
32	36.022359514958	38.0	35.0	40.0	30.0	41.0
33	35.89117376631337	38.0	35.0	40.0	30.0	41.0
34	35.79704724420811	38.0	35.0	40.0	30.0	41.0
35	35.680725704466994	38.0	35.0	40.0	30.0	41.0
36	35.54415136985867	38.0	35.0	40.0	29.0	41.0
37	35.413093755970245	37.0	34.0	40.0	28.0	41.0
38	35.33505853507343	37.0	34.0	40.0	28.0	41.0
39	35.28272818520205	37.0	34.0	40.0	28.0	41.0
40	35.115959645632365	37.0	34.0	40.0	27.0	41.0
41	35.066564851482006	37.0	34.0	40.0	27.0	41.0
42	34.962629094323916	37.0	34.0	40.0	26.0	41.0
43	34.8715524102898	37.0	34.0	40.0	26.0	41.0
44	34.68425806801647	36.0	34.0	40.0	26.0	41.0
45	34.55350751579607	36.0	34.0	40.0	25.0	41.0
46	34.48520303298431	36.0	34.0	40.0	25.0	41.0
47	34.409532533241446	36.0	34.0	40.0	24.0	41.0
48	34.33127740539817	35.0	34.0	40.0	24.0	41.0
49	34.2069330138045	36.0	34.0	40.0	24.0	41.0
50	34.09487455203339	35.0	33.0	40.0	24.0	41.0
51	33.737581750840235	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	2.0
13	4.0
14	9.0
15	51.0
16	108.0
17	279.0
18	666.0
19	1255.0
20	2345.0
21	3845.0
22	5926.0
23	8733.0
24	12852.0
25	18030.0
26	23727.0
27	27146.0
28	30685.0
29	34820.0
30	41435.0
31	50292.0
32	62900.0
33	82787.0
34	154985.0
35	249098.0
36	117039.0
37	149330.0
38	237857.0
39	416274.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.35405422408922	23.971184686626827	29.75687295785232	12.91788813143163
2	30.20032310737173	26.514140132094237	30.170540434307565	13.114996326226471
3	29.194176679358936	25.189850111194417	31.811646525845415	13.804326683601234
4	25.60294040406314	28.255791604402987	30.623225521211207	15.518042470322662
5	23.375069478474227	32.603888716695785	28.917013121114472	15.104028683715514
6	21.448661540122053	41.39514507798039	26.322168871219375	10.834024510678185
7	88.65568748048398	3.928311489820502	5.7047671323961024	1.7112338972994159
8	89.17803862627997	3.1683915798149664	5.149401085451453	2.504168708453604
9	84.53984904340479	5.571610878987112	7.01820610186944	2.870333975738665
10	47.45482239772451	29.978280581639442	11.882074467071964	10.68482255356408
11	40.27887202166055	21.23146735651649	22.380582158909032	16.109078462913935
12	37.72235276191004	20.554835038045056	25.400926148782922	16.321886051261984
13	22.90916688743939	35.754907070556655	25.439655167438076	15.896270874565884
14	16.915981116861783	38.99371504792644	26.435527727610904	17.654776107600874
15	14.637952937604723	26.138105025479767	43.04329280927372	16.180649227641783
16	17.69287022431086	21.441735337083873	41.556987355639166	19.3084070829661
17	17.929919523292533	22.197326370190545	26.835689108141693	33.03706499837523
18	21.805130123153663	24.92284498407262	32.42219132366089	20.84983356911283
19	26.376655001786382	26.80850376121684	25.244624833554685	21.570216403442092
20	29.458238170189503	24.411344889703102	25.2937431567671	20.836673783340288
21	22.582769569265203	28.501152347030477	28.038828294232033	20.877249789472287
22	22.933812626583574	24.176431169991535	26.170946771552465	26.718809431872426
23	20.077561930355873	29.29783885149701	24.925326873494633	25.699272344652478
24	20.71633100555193	25.477633847430752	34.56700553115031	19.239029615867008
25	19.15903197077604	26.507444802490664	31.580080470935634	22.753442755797664
26	19.213344946267092	32.904197336759495	25.704524715289768	22.177933001683645
27	18.958633829538062	32.95660560641505	27.869886658459116	20.214873905587773
28	16.886025288721658	28.65075833265514	35.21027663832118	19.252939740302015
29	17.646637819031014	24.82616673333137	35.26464733217088	22.262548115466732
30	20.20154096473928	29.37668212941495	30.743048833771702	19.67872807207407
31	27.441154690395837	27.331085780447445	24.360321860655183	20.867437668501534
32	28.129907864184084	27.37795308767246	25.595148425645192	18.89699062249827
33	25.84553066547536	27.674740887858423	25.987517827758026	20.49221061890819
34	20.444881564813965	27.493736115127348	27.766282204679687	24.295100115378997
35	19.787354023056174	25.728535552488786	30.489145774063793	23.994964650391243
36	28.483375092565815	24.253889207301835	28.39223780425511	18.870497895877236
37	21.05611898293326	28.36355178000532	30.118420756445264	20.461908480616156
38	21.07678215533049	29.51295517419112	25.8046660675501	23.605596602928284
39	19.8600214365984	28.98985368973265	28.659646959887475	22.49047791378147
40	23.324392759578217	25.122665941724083	27.326410593396673	24.226530705301027
41	18.7510786118273	23.56715617606639	29.229731188288255	28.452034023818058
42	22.980275905298026	25.55168650158062	26.335790403861125	25.13224718926023
43	23.005094799518165	25.464185469864958	27.688997322445342	23.841722408171535
44	20.869457811054335	29.007804099273265	27.98197571096031	22.140762378712083
45	19.681325398213385	32.86050453926032	23.714280272269043	23.743889790257256
46	22.94264353545725	29.58643064475447	26.54986779609951	20.92105802368877
47	21.886108980341703	26.938543223258655	27.551685347213446	23.6236624491862
48	22.614110638012967	24.526723888647435	29.872713703665866	22.986451769673735
49	21.53218000509076	22.916439400629475	31.120353744276503	24.431026850003263
50	19.781005003604513	28.472985788008547	29.198909584768355	22.547099623618582
51	18.55720264511694	29.983128923766166	26.590963267459372	24.86870516365752
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1246.0
1	1792.5
2	2339.0
3	6129.5
4	9920.0
5	7642.5
6	5365.0
7	5455.5
8	5546.0
9	6037.0
10	6528.0
11	6854.0
12	7180.0
13	7024.0
14	6868.0
15	6725.5
16	6583.0
17	6245.5
18	5908.0
19	6080.0
20	6252.0
21	6233.5
22	6215.0
23	6325.5
24	6436.0
25	8418.5
26	11507.0
27	12613.0
28	15180.0
29	17747.0
30	20255.5
31	22764.0
32	29377.0
33	35990.0
34	41601.0
35	47212.0
36	51240.5
37	55269.0
38	63063.0
39	70857.0
40	86976.5
41	103096.0
42	125623.0
43	148150.0
44	154373.5
45	160597.0
46	161096.5
47	161596.0
48	159215.5
49	156835.0
50	147697.5
51	138560.0
52	125061.0
53	111562.0
54	97233.5
55	82905.0
56	79382.5
57	75860.0
58	69863.5
59	63867.0
60	59087.5
61	54308.0
62	48503.5
63	42699.0
64	36455.0
65	30211.0
66	25096.5
67	19982.0
68	16539.5
69	13097.0
70	10961.0
71	8825.0
72	7182.0
73	5539.0
74	4216.5
75	2192.5
76	1491.0
77	990.5
78	490.0
79	406.0
80	322.0
81	288.0
82	254.0
83	167.5
84	81.0
85	62.0
86	43.0
87	34.5
88	26.0
89	21.5
90	17.0
91	9.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1732551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.3491060071133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91118868830205	15.26860955077276
2	7.991782925856755	3.0926771003648086
3	2.763368109413363	1.6040610745714625
4	1.332311026728974	1.031161091624995
5	0.7759035778345509	0.7506520289409604
6	0.5260084409467516	0.6106675850709055
7	0.36936063176789313	0.5002758613251913
8	0.29444968501837354	0.4557870535345304
9	0.23230140915902123	0.40453421322777305
>10	3.3434508715174482	17.022305636995338
>50	2.6650185661460752	38.03807002497516
>100	0.7864033632129867	19.34157434322641
>500	0.005735763493633966	0.7096416068027829
>1k	0.002415058313109038	0.8237777249765121
>5k	3.0188228913862977E-4	0.3462051035904267
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5927	0.3420967117273893	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4733	0.2731809914975086	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10822192247154629	0.0
2	0.0	0.0	0.0	0.39785264618472993	0.0
3	0.0	0.0	0.0	0.5492479009275918	0.0
4	0.0	0.0	0.0	0.8744908519287455	0.0
5	0.0	0.0	0.0	1.4209682716410657	0.0
6	0.0	0.0	0.0	2.0165062962071536	0.0
7	0.0	0.0	0.0	2.3141598717728944	0.0
8	0.0	0.0	0.0	3.057341457769497	0.0
9	0.0	0.0	0.0	3.31055189717359	0.0
10	5.771835865149136E-5	0.0	0.0	3.8151835068635784	0.0
11	5.771835865149136E-5	0.0	0.0	4.490892331596588	0.0
12	5.771835865149136E-5	0.0	0.0	4.993157488581866	0.0
13	5.771835865149136E-5	0.0	0.0	5.180568999123258	0.0
14	5.771835865149136E-5	0.0	0.0	5.250639086526169	0.0
15	5.771835865149136E-5	0.0	0.0	5.373232880301936	0.0
16	5.771835865149136E-5	0.0	0.0	5.652878327968412	0.0
17	5.771835865149136E-5	0.0	0.0	5.970733329062175	0.0
18	5.771835865149136E-5	0.0	0.0	6.398772676821634	0.0
19	5.771835865149136E-5	0.0	0.0	6.600671495384551	0.0
20	5.771835865149136E-5	0.0	0.0	6.814171704036418	0.0
21	1.1543671730298271E-4	0.0	0.0	7.093066813040425	0.0
22	1.1543671730298271E-4	0.0	0.0	7.372654542348248	0.0
23	1.1543671730298271E-4	0.0	0.0	7.676368545572396	0.0
24	1.1543671730298271E-4	0.0	0.0	7.8914271499078525	0.0
25	1.1543671730298271E-4	0.0	0.0	8.065967466469962	0.0
26	1.1543671730298271E-4	0.0	0.0	8.24778029622216	0.0
27	1.1543671730298271E-4	0.0	0.0	8.423763571750557	0.0
28	1.1543671730298271E-4	0.0	0.0	8.612848914692844	0.0
29	1.1543671730298271E-4	0.0	0.0	8.812612153985654	0.0
30	1.1543671730298271E-4	0.0	0.0	9.077712575271955	0.0
31	1.1543671730298271E-4	0.0	0.0	9.300274566232105	0.0
32	1.1543671730298271E-4	0.0	0.0	9.50130760941525	0.0
33	1.1543671730298271E-4	0.0	0.0	9.713191704024874	0.0
34	1.1543671730298271E-4	0.0	0.0	9.930328169271785	0.0
35	1.1543671730298271E-4	0.0	0.0	10.207376290798944	0.0
36	1.1543671730298271E-4	0.0	0.0	10.422204021699795	0.0
37	1.1543671730298271E-4	0.0	0.0	10.644073392356127	0.0
38	1.1543671730298271E-4	0.0	0.0	10.862768253286628	0.0
39	1.1543671730298271E-4	0.0	0.0	11.12913847846326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	70	0.0	45.000004	1
CGTTCAA	25	3.891628E-5	45.0	9
TCGACGA	25	3.891628E-5	45.0	34
ATACTCG	135	0.0	43.333336	44
GCGATAC	80	0.0	42.1875	9
TAATGCG	70	0.0	41.785717	1
CGGGACC	555	0.0	41.756756	6
CGTTTTT	3580	0.0	41.166203	1
TACGGGA	575	0.0	40.695652	4
CGACGGT	305	0.0	40.57377	28
CGTAAGG	200	0.0	40.5	2
TAACGCC	190	0.0	40.26316	12
GATACGT	45	1.9292202E-8	40.0	11
ATACGTA	45	1.9292202E-8	40.0	12
TCACGAC	315	0.0	39.999996	25
TAAGGGA	1865	0.0	39.571045	4
CGCATCG	165	0.0	39.545452	21
CGGTCTA	300	0.0	39.000004	31
AAATGCG	75	0.0	39.000004	1
ACGTAGG	295	0.0	38.898304	2
>>END_MODULE