FastQCFastQC Report
Thu 26 May 2016
SRR1547913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547913_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1984062
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85810.43249656512750106No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49700.2504962042516816No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT207.034525E-445.00000421
GAGTCGA207.034525E-445.00000411
ACCTCGT207.034525E-445.00000411
TCTAACG207.034525E-445.0000041
CTCGCTA406.8193913E-945.00000431
CGTAATC207.034525E-445.00000419
CGGTCGA207.034525E-445.00000443
CACGTAT207.034525E-445.00000429
AGTCGAC207.034525E-445.00000412
GTATCCG253.891834E-545.044
TGTCGAC253.891834E-545.026
TCGGTAT502.1827873E-1145.042
CCCGATA253.891834E-545.023
GCCGAAT302.1663036E-644.99999639
CGTAGAA302.1663036E-644.99999639
CGAATCC302.1663036E-644.99999641
CGACGGT3450.043.69565228
CGGTCTA3500.042.4285731
GCTCGAT750.042.044
TAATACG650.041.5384641