##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547913_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1984062
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.483705146310953	31.0	31.0	33.0	30.0	34.0
2	31.899845367735484	31.0	31.0	34.0	30.0	34.0
3	31.9832878206427	33.0	31.0	34.0	30.0	34.0
4	35.73728240347328	37.0	35.0	37.0	35.0	37.0
5	35.64253788440079	37.0	35.0	37.0	33.0	37.0
6	35.67695565965177	37.0	35.0	37.0	33.0	37.0
7	36.00752194235866	37.0	35.0	37.0	35.0	37.0
8	36.03228528140754	37.0	35.0	37.0	35.0	37.0
9	37.82623627689054	39.0	38.0	39.0	35.0	39.0
10	37.309646573544576	39.0	37.0	39.0	34.0	39.0
11	37.029157858978195	39.0	37.0	39.0	33.0	39.0
12	36.2572076880662	38.0	35.0	39.0	32.0	39.0
13	35.9613676387129	38.0	35.0	39.0	32.0	39.0
14	36.92452504004411	39.0	35.0	41.0	32.0	41.0
15	37.19497273774711	39.0	35.0	41.0	32.0	41.0
16	37.33159800449784	39.0	35.0	41.0	33.0	41.0
17	37.26801884215312	39.0	35.0	41.0	33.0	41.0
18	37.210490397981516	39.0	35.0	41.0	32.0	41.0
19	37.12029210780712	38.0	35.0	41.0	32.0	41.0
20	36.9587301203289	38.0	35.0	41.0	32.0	41.0
21	36.83641993042556	38.0	35.0	41.0	32.0	41.0
22	36.73712817442197	38.0	35.0	41.0	32.0	41.0
23	36.68159109947169	38.0	35.0	41.0	32.0	41.0
24	36.690149803786376	38.0	35.0	41.0	32.0	41.0
25	36.626729910658035	38.0	35.0	41.0	32.0	41.0
26	36.49824753460325	38.0	35.0	40.0	31.0	41.0
27	36.42257500017641	38.0	35.0	40.0	31.0	41.0
28	36.39488836538374	38.0	35.0	40.0	31.0	41.0
29	36.41025734074842	38.0	35.0	40.0	31.0	41.0
30	36.324371415812614	38.0	35.0	40.0	31.0	41.0
31	36.1359881898852	38.0	35.0	40.0	30.0	41.0
32	35.96704135253838	38.0	35.0	40.0	30.0	41.0
33	35.857757469272634	38.0	35.0	40.0	30.0	41.0
34	35.765429205337334	38.0	35.0	40.0	30.0	41.0
35	35.6753019814905	38.0	35.0	40.0	30.0	41.0
36	35.52703897358046	38.0	35.0	40.0	29.0	41.0
37	35.40649536153608	37.0	34.0	40.0	28.0	41.0
38	35.30808462638768	37.0	34.0	40.0	28.0	41.0
39	35.22823732322881	37.0	34.0	40.0	27.0	41.0
40	35.05466915852428	37.0	34.0	40.0	26.0	41.0
41	35.01216897455826	37.0	34.0	40.0	26.0	41.0
42	34.92238044980449	37.0	34.0	40.0	26.0	41.0
43	34.84270098414263	37.0	34.0	40.0	26.0	41.0
44	34.67847224532298	36.0	34.0	40.0	25.0	41.0
45	34.514294412170585	36.0	34.0	40.0	24.0	41.0
46	34.47273371497463	36.0	34.0	40.0	24.0	41.0
47	34.38579489955455	36.0	34.0	40.0	24.0	41.0
48	34.30664465122562	36.0	34.0	40.0	24.0	41.0
49	34.19726046867487	36.0	34.0	40.0	24.0	41.0
50	34.09532766617172	35.0	34.0	40.0	24.0	41.0
51	33.766299137829364	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	1.0
13	6.0
14	21.0
15	53.0
16	158.0
17	427.0
18	944.0
19	1545.0
20	2775.0
21	4559.0
22	7155.0
23	10558.0
24	15346.0
25	21567.0
26	27500.0
27	31832.0
28	35858.0
29	41532.0
30	48433.0
31	58565.0
32	72252.0
33	95865.0
34	174940.0
35	286363.0
36	129317.0
37	165552.0
38	262868.0
39	487977.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.115799808675334	24.58476600025604	29.6519463605472	12.647487830521426
2	29.21264557256779	26.59937038257877	31.054120284547558	13.133863760305879
3	29.366673017274664	25.756805986909683	31.357588623742604	13.518932372073051
4	25.579946594410863	28.592402858378417	30.8002471696953	15.02740337751542
5	23.68439091117112	32.27132015027756	29.85199051239326	14.192298426158054
6	20.969102780054254	41.81729199994758	26.50995785413964	10.703647365858526
7	88.91400571151506	3.855423872842683	5.747249833926561	1.4833205817156925
8	89.78494623655914	2.909586494776877	4.9491900958740205	2.356277172789963
9	86.09569660625525	4.752774862882309	6.727763547711714	2.423764983150728
10	46.57908875831501	28.199673195696505	12.7052481222865	12.515989923701982
11	39.61998163363846	24.189717861639405	21.574124195715658	14.616176309006473
12	37.2595715254866	21.546655296054258	24.884605420596735	16.309167757862404
13	21.83540635322888	36.46897123174578	25.56855582134026	16.127066593685075
14	15.277193958656534	39.51983355358855	28.090906433367508	17.112066054387412
15	14.108430079301955	25.871620947329266	44.28541043576259	15.734538537606182
16	17.46991777474696	21.898005203466425	41.692850324233824	18.9392266975528
17	17.561951189025343	22.63376850118595	27.504080013628606	32.3002002961601
18	21.135428227545308	25.62697133456515	32.80204953272629	20.435550905163247
19	25.987242334160925	27.910972540172637	25.32839195549333	20.77339317017311
20	27.74368946131724	26.284057655456333	25.89455369842273	20.077699184803702
21	21.757838212717143	28.97933633122352	29.127113971236785	20.13571148482255
22	22.68336372552874	24.107915982464256	27.437398629679922	25.771321662327086
23	19.45428116661677	29.685261851696165	26.615246902566554	24.24521007912051
24	19.935213718119694	26.232849578289386	35.48931434602346	18.342622357567457
25	18.99310606220975	27.189321704664472	32.3064501008537	21.511122132272074
26	17.7422378937755	33.86915328250831	26.590046077189122	21.798562746527075
27	17.712803329734655	33.06408771500084	29.144804950651743	20.07830400461276
28	15.733631307892596	29.448323691497546	36.312474106151925	18.505570894457936
29	16.47236830300666	26.214452975763862	35.24587437287746	22.067304348352017
30	19.256706695657698	29.665504404600256	32.14521522008889	18.932573679653157
31	27.175058037500843	27.447831771386177	25.931346903473784	19.4457632876392
32	26.950266675134145	27.839906212608273	26.355980811083523	18.853846301174055
33	25.360245798770404	27.896910479612032	26.329318337834202	20.41352538378337
34	19.529934044399823	27.685475554695365	28.70883067162216	24.07575972928265
35	19.48941111719291	26.43606903413301	30.126477902404257	23.948041946269825
36	27.60926825875401	24.558708346815774	27.67191751064231	20.160105883787907
37	19.704525362614678	29.554671174590307	30.786033904182432	19.954769558612583
38	20.304405809899087	30.708314558718424	25.97474272477372	23.012536906608766
39	19.500449078708225	29.03860867251124	28.75766987120362	22.703272377576912
40	22.94782118703952	25.503537691866484	27.18110623559143	24.36753488550257
41	17.926808738839814	23.810495841359796	29.515257083700003	28.747438336100384
42	21.67059295526047	25.29124593888699	26.810452495940147	26.22770860991239
43	21.548671362084452	25.988048760573008	28.077802004171243	24.3854778731713
44	20.23485153185737	29.214964048502516	27.868282341983264	22.681902077656847
45	18.50955262486757	33.17648339618419	24.654421081599263	23.659542897348974
46	22.94545230945404	29.160127052481222	27.018359305303967	20.87606133276077
47	20.752426083459085	26.811510930605998	28.814472531604356	23.62159045433056
48	21.544689631674814	24.734710911251764	30.7826065919311	22.93799286514232
49	20.639727992371206	23.848599489330475	31.393525000730826	24.118147517567497
50	19.582502966137145	28.81936149172758	29.340212150628357	22.257923391506917
51	18.50592370601322	30.381863066779164	26.179575033441495	24.93263819376612
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1674.0
1	2338.0
2	3002.0
3	8290.5
4	13579.0
5	10176.5
6	6774.0
7	6800.5
8	6827.0
9	7487.5
10	8148.0
11	8381.5
12	8615.0
13	8717.0
14	8819.0
15	8402.0
16	7985.0
17	7980.0
18	7975.0
19	8250.0
20	8525.0
21	8554.5
22	8584.0
23	8824.5
24	9065.0
25	10761.5
26	14522.5
27	16587.0
28	20922.5
29	25258.0
30	28101.5
31	30945.0
32	36980.0
33	43015.0
34	50968.5
35	58922.0
36	61609.5
37	64297.0
38	71074.5
39	77852.0
40	103450.0
41	129048.0
42	153347.0
43	177646.0
44	181602.5
45	185559.0
46	186885.0
47	188211.0
48	187083.5
49	185956.0
50	170931.5
51	155907.0
52	140527.0
53	125147.0
54	112139.0
55	99131.0
56	91613.5
57	84096.0
58	75189.0
59	66282.0
60	59765.5
61	53249.0
62	44353.0
63	35457.0
64	31042.0
65	26627.0
66	22045.0
67	17463.0
68	13734.0
69	10005.0
70	8338.0
71	6671.0
72	5581.5
73	4492.0
74	3286.5
75	1561.0
76	1041.0
77	767.0
78	493.0
79	361.5
80	230.0
81	202.5
82	175.0
83	136.5
84	98.0
85	73.0
86	48.0
87	36.0
88	24.0
89	17.0
90	10.0
91	7.0
92	4.0
93	4.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1984062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.198091546593915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9397674944533	14.962947914787591
2	8.997818871535182	3.4548190083080557
3	3.2699732797362833	1.8833173913787933
4	1.5787408270223409	1.2123524370208114
5	0.9314872260600198	0.8941388520191541
6	0.5966690830932514	0.6872944608147895
7	0.41841245297113755	0.5622904403462374
8	0.3126248512914601	0.4801440411867008
9	0.25103138712809553	0.4337391196038271
>10	2.522931257172202	11.774902802232763
>50	1.8040767213674571	26.040710049053633
>100	1.3647587329550719	35.19933829617963
>500	0.009046948120075341	1.1848834076951424
>1k	0.0023947803847258254	0.7907736646312052
>5k	2.6608670941398064E-4	0.43834811474162616
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8581	0.43249656512750106	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4970	0.2504962042516816	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08895891358233765	0.0
2	0.0	0.0	0.0	0.30457717551165236	0.0
3	5.040165075486552E-5	0.0	0.0	0.443383321690552	0.0
4	5.040165075486552E-5	0.0	0.0	0.7659034848709365	0.0
5	5.040165075486552E-5	0.0	0.0	1.2418462729491315	0.0
6	5.040165075486552E-5	0.0	0.0	1.8742861866211842	0.0
7	5.040165075486552E-5	0.0	0.0	2.1473119287602906	0.0
8	5.040165075486552E-5	0.0	0.0	2.8534390558359566	0.0
9	5.040165075486552E-5	0.0	0.0	3.105497711261039	0.0
10	5.040165075486552E-5	0.0	0.0	3.5944945268847444	0.0
11	5.040165075486552E-5	0.0	0.0	4.2458854612406265	0.0
12	5.040165075486552E-5	0.0	0.0	4.753430084342122	0.0
13	5.040165075486552E-5	0.0	0.0	4.966881075288978	0.0
14	5.040165075486552E-5	0.0	0.0	5.056545611981884	0.0
15	5.040165075486552E-5	0.0	0.0	5.189404363371709	0.0
16	5.040165075486552E-5	0.0	0.0	5.492116677805432	0.0
17	5.040165075486552E-5	0.0	0.0	5.835906337604369	0.0
18	5.040165075486552E-5	0.0	0.0	6.299349516295358	0.0
19	5.040165075486552E-5	0.0	0.0	6.520259951553934	0.0
20	5.040165075486552E-5	0.0	0.0	6.746714568395544	0.0
21	5.040165075486552E-5	0.0	0.0	7.060968860852131	0.0
22	5.040165075486552E-5	0.0	0.0	7.369275758519643	0.0
23	5.040165075486552E-5	0.0	0.0	7.732671660462223	0.0
24	5.040165075486552E-5	0.0	0.0	7.979841355764084	0.0
25	5.040165075486552E-5	0.0	0.0	8.177214220120137	0.0
26	5.040165075486552E-5	0.0	0.0	8.373075034953544	0.0
27	5.040165075486552E-5	0.0	0.0	8.579873008000758	0.0
28	5.040165075486552E-5	0.0	0.0	8.786318169492688	0.0
29	5.040165075486552E-5	0.0	0.0	9.031421397113599	0.0
30	5.040165075486552E-5	0.0	0.0	9.325968644125032	0.0
31	5.040165075486552E-5	0.0	0.0	9.59093012214336	0.0
32	5.040165075486552E-5	0.0	0.0	9.824037756884614	0.0
33	5.040165075486552E-5	0.0	0.0	10.05553253880171	0.0
34	5.040165075486552E-5	0.0	0.0	10.320090803614	0.0
35	5.040165075486552E-5	0.0	0.0	10.652993706849887	0.0
36	5.040165075486552E-5	0.0	0.0	10.919114422835577	0.0
37	5.040165075486552E-5	0.0	0.0	11.183269474441827	0.0
38	5.040165075486552E-5	0.0	0.0	11.451456658108466	0.0
39	5.040165075486552E-5	0.0	0.0	11.835265228606767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAT	20	7.034525E-4	45.000004	21
GAGTCGA	20	7.034525E-4	45.000004	11
ACCTCGT	20	7.034525E-4	45.000004	11
TCTAACG	20	7.034525E-4	45.000004	1
CTCGCTA	40	6.8193913E-9	45.000004	31
CGTAATC	20	7.034525E-4	45.000004	19
CGGTCGA	20	7.034525E-4	45.000004	43
CACGTAT	20	7.034525E-4	45.000004	29
AGTCGAC	20	7.034525E-4	45.000004	12
GTATCCG	25	3.891834E-5	45.0	44
TGTCGAC	25	3.891834E-5	45.0	26
TCGGTAT	50	2.1827873E-11	45.0	42
CCCGATA	25	3.891834E-5	45.0	23
GCCGAAT	30	2.1663036E-6	44.999996	39
CGTAGAA	30	2.1663036E-6	44.999996	39
CGAATCC	30	2.1663036E-6	44.999996	41
CGACGGT	345	0.0	43.695652	28
CGGTCTA	350	0.0	42.42857	31
GCTCGAT	75	0.0	42.0	44
TAATACG	65	0.0	41.538464	1
>>END_MODULE