FastQCFastQC Report
Thu 26 May 2016
SRR1547912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547912_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2071338
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408951.9743277050872432No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT48790.23554823017778848No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41340.19958114030641066No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC38950.1880427047637807No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT29480.1423234643500964No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG24600.11876381353501939TruSeq Adapter, Index 13 (95% over 21bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24250.11707408448065935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT177950.043.5585821
TTGCACG800.042.1875041
TAAGACG650.041.538461
TACGGGA8100.041.111114
TTTAGCG1400.040.178571
ACGGGAT11600.038.987075
TAGGGAC26500.038.886795
GCGCGAC6500.038.769239
CGGTTTT16450.038.7082061
TAGGGAT33250.038.5037575
CGAGGGA16550.038.3383674
ATAGGGA29600.038.23484
AAGGGAT35750.037.8881155
CGATCGA601.5643309E-1037.50000410
CGGTCTA2700.037.531
CGACGGT2900.037.2413828
CTAGGGA15200.037.1546064
AGGGATC22750.036.9890146
TAAGGGA28750.036.939134
GCGTTAG1950.036.9230771