##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547912_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2071338
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.427424205996317	31.0	31.0	33.0	30.0	34.0
2	31.790046819978198	31.0	31.0	34.0	30.0	34.0
3	31.542836562646947	31.0	31.0	34.0	30.0	34.0
4	35.51397840429713	37.0	35.0	37.0	33.0	37.0
5	35.54388516022011	37.0	35.0	37.0	33.0	37.0
6	35.63183121248198	37.0	35.0	37.0	33.0	37.0
7	35.99126023855112	37.0	35.0	37.0	35.0	37.0
8	36.02733305718333	37.0	35.0	37.0	35.0	37.0
9	37.77995382694664	39.0	38.0	39.0	35.0	39.0
10	37.32679553023215	39.0	37.0	39.0	34.0	39.0
11	37.07856322821287	39.0	37.0	39.0	33.0	39.0
12	36.849385276570025	39.0	35.0	39.0	33.0	39.0
13	36.80403391431046	39.0	35.0	39.0	33.0	39.0
14	37.95923649351289	40.0	37.0	41.0	33.0	41.0
15	38.06862665581378	40.0	37.0	41.0	33.0	41.0
16	38.102827737433486	40.0	37.0	41.0	33.0	41.0
17	37.994354856619246	40.0	36.0	41.0	33.0	41.0
18	37.79957592628533	39.0	36.0	41.0	33.0	41.0
19	37.620516303954254	39.0	36.0	41.0	33.0	41.0
20	37.36864142887351	39.0	35.0	41.0	32.0	41.0
21	37.276428086579784	39.0	35.0	41.0	32.0	41.0
22	37.19051791643855	39.0	35.0	41.0	32.0	41.0
23	37.073872540357975	39.0	35.0	41.0	32.0	41.0
24	37.08408912500036	39.0	35.0	41.0	32.0	41.0
25	37.050316751780734	39.0	35.0	41.0	32.0	41.0
26	36.937851765380636	39.0	35.0	41.0	32.0	41.0
27	36.85982104320975	39.0	35.0	41.0	31.0	41.0
28	36.751296987744155	39.0	35.0	41.0	31.0	41.0
29	36.68085942516383	38.0	35.0	41.0	31.0	41.0
30	36.53594826146192	38.0	35.0	41.0	31.0	41.0
31	36.32769350052961	38.0	35.0	41.0	30.0	41.0
32	36.086008657206115	38.0	35.0	41.0	30.0	41.0
33	35.83540494115398	38.0	35.0	41.0	28.0	41.0
34	35.53387955031965	38.0	35.0	41.0	26.0	41.0
35	35.31617727285455	38.0	35.0	41.0	25.0	41.0
36	35.17128155810399	38.0	35.0	40.0	24.0	41.0
37	35.0701845860019	38.0	34.0	40.0	24.0	41.0
38	34.90687420401692	38.0	34.0	40.0	23.0	41.0
39	34.79943060958665	38.0	34.0	40.0	23.0	41.0
40	34.62324159552907	38.0	34.0	40.0	22.0	41.0
41	34.49315370065146	38.0	34.0	40.0	21.0	41.0
42	34.38995277448683	38.0	34.0	40.0	21.0	41.0
43	34.29054649699856	38.0	33.0	40.0	20.0	41.0
44	34.055592568668175	38.0	33.0	40.0	18.0	41.0
45	33.90538193187206	37.0	33.0	40.0	18.0	41.0
46	33.849296927879465	37.0	33.0	40.0	18.0	41.0
47	33.748038224567885	37.0	33.0	40.0	18.0	41.0
48	33.6172140906023	37.0	33.0	40.0	18.0	41.0
49	33.45607090682448	37.0	33.0	40.0	18.0	41.0
50	33.37748885020214	37.0	33.0	40.0	18.0	41.0
51	33.04120766383854	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	7.0
13	6.0
14	19.0
15	47.0
16	132.0
17	337.0
18	773.0
19	1674.0
20	2911.0
21	4696.0
22	7632.0
23	12286.0
24	19756.0
25	31066.0
26	43164.0
27	48732.0
28	48193.0
29	47877.0
30	51818.0
31	58611.0
32	69906.0
33	87864.0
34	129873.0
35	180031.0
36	154445.0
37	199662.0
38	314574.0
39	555167.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.977193485563436	22.70546863911153	26.9176735037932	20.39966437153183
2	36.20543822398855	23.751845425517224	27.31036653602647	12.73234981446775
3	27.778131816246308	24.07786657706275	35.20830497002421	12.935696636666735
4	24.07950802814413	25.68619896897561	35.71174767227753	14.522545330602732
5	21.5765365189071	29.780460745662946	34.08946294617296	14.553539789256991
6	19.762443406146172	38.04917401216026	31.166279960103083	11.02210262159049
7	82.0734230724295	2.622266380474843	13.055088063850514	2.2492224832451293
8	82.31196453693217	2.068035250644752	12.713038625275065	2.9069615871480172
9	77.00249790232208	4.6356509657042935	14.908479446618564	3.45337168535507
10	34.3502122782472	35.69629872092339	19.06772337493929	10.885765625890125
11	24.006849678806645	23.519000761826415	34.74121558142611	17.732933977940828
12	24.62953897432481	21.00318731177625	35.40383076060015	18.963442953298788
13	21.905068125047674	23.41838946613252	36.14200096749058	18.534541441329228
14	16.608057207466864	28.03516374440096	35.80893123188972	19.54784781624245
15	16.004292877357535	26.86104344148565	39.35673463239703	17.777929048759788
16	19.780837313852206	24.62418011932384	38.37152603775917	17.223456529064787
17	18.858052138279703	25.31807942499003	34.134120071181044	21.689748365549224
18	19.597574128413616	26.337951604228767	34.86046217469095	19.204012092666673
19	21.589378459720237	28.513019120973983	31.512722694219867	18.384879725085913
20	22.90519461333689	27.163456664243114	30.932711126817548	18.998637595602457
21	21.78553186394495	27.062652256657294	33.336374845631184	17.81544103376658
22	20.39058811261127	23.387057061667385	33.470008274844574	22.752346550876776
23	18.468931676047077	27.840024177608868	33.03217533787339	20.65886880847066
24	18.842361797060644	26.488240934120842	35.5938045842832	19.075592684535312
25	21.075942217059698	26.835166447967453	32.46795066763609	19.620940667336765
26	18.863748939091543	28.85057870806213	31.556269425849376	20.729402926996947
27	19.140912781979573	26.385650241534698	33.996865793994026	20.476571182491703
28	18.5343483294373	26.84245642188769	35.30630925517709	19.316885993497923
29	18.58079173944571	25.86922076454929	34.843323494282444	20.706664001722558
30	19.863634037515848	24.77123482502614	35.36945684383717	19.995674293620837
31	21.414708753472393	25.353901680942464	31.85047539319995	21.380914172385193
32	20.93303941703382	25.76928536047714	33.469477217141765	19.828198005347268
33	19.745353003710644	25.491783571778242	32.55407857143547	22.208784853075645
34	17.724050830912194	26.92520486757835	33.78217364814434	21.568570653365118
35	17.80308187268326	25.774306269667242	32.91524608731168	23.50736577033782
36	20.032751776870796	27.391521808608736	30.36056886901124	22.215157545509232
37	18.445468581177963	27.434344370643515	30.87888118694293	23.24130586123559
38	18.801518631918114	28.06929627129903	30.213900387092785	22.915284709690063
39	18.678602912706666	26.559112998458	29.204021748261265	25.55826234057406
40	21.044271866783692	25.519108904485893	31.476224546645692	21.96039468208472
41	18.48220811861705	25.988660469706055	31.637521254377603	23.891610157299294
42	20.57747214602349	27.80618131854869	29.3969405282962	22.219406007131624
43	20.41709271977823	26.817400153910175	30.676065422446747	22.089441703864846
44	21.030271254618995	27.7694417811096	30.33498154333093	20.865305420940476
45	19.761333012767594	27.159304758566684	30.180395473843475	22.898966754822244
46	21.252687876145757	26.825124629587254	29.77481222282409	22.147375271442904
47	20.873802344185254	27.07047328828033	30.40474321428951	21.650981153244906
48	19.83500519953769	26.43373510262449	30.836203458827093	22.895056239010728
49	20.19279325730518	25.79057594656208	30.687700413935342	23.328930382197402
50	18.261481226144646	26.059146310259358	33.49434037322735	22.185032090368644
51	18.46255898361349	26.22039474001829	31.852647901984128	23.464398374384093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1299.0
1	1919.5
2	2540.0
3	31776.0
4	61012.0
5	42957.5
6	24903.0
7	22030.5
8	19158.0
9	19318.5
10	19479.0
11	19724.5
12	19970.0
13	19356.5
14	18743.0
15	17886.0
16	17029.0
17	16089.0
18	15149.0
19	14709.5
20	14270.0
21	14251.5
22	14233.0
23	14568.0
24	14903.0
25	16428.0
26	20697.0
27	23441.0
28	26963.5
29	30486.0
30	35037.5
31	39589.0
32	44391.0
33	49193.0
34	55776.5
35	62360.0
36	66403.5
37	70447.0
38	76734.5
39	83022.0
40	90525.0
41	98028.0
42	108212.5
43	118397.0
44	128065.0
45	137733.0
46	157956.0
47	178179.0
48	185144.5
49	192110.0
50	180049.0
51	167988.0
52	147552.0
53	127116.0
54	112459.0
55	97802.0
56	89655.0
57	81508.0
58	76579.5
59	71651.0
60	64552.5
61	57454.0
62	49692.0
63	41930.0
64	35535.0
65	29140.0
66	23431.0
67	17722.0
68	14887.5
69	12053.0
70	10325.5
71	8598.0
72	7648.5
73	6699.0
74	5575.0
75	3135.0
76	1819.0
77	1389.5
78	960.0
79	713.0
80	466.0
81	321.5
82	177.0
83	127.0
84	77.0
85	66.0
86	55.0
87	39.0
88	23.0
89	18.5
90	14.0
91	9.5
92	5.0
93	2.5
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2071338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.48363926228717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1767735527682	21.280339545456062
2	7.76577029400022	4.579263398841765
3	3.492772829308948	3.0893896237338945
4	2.1604154251551653	2.547876362078226
5	1.5843107877138667	2.3355623872152833
6	1.2841021721276304	2.2716003131358264
7	1.0704428795249987	2.209238619755929
8	0.9222800153238142	2.1753737016499217
9	0.8447811091182079	2.2416499329152484
>10	8.512973767855046	48.359808804541586
>50	0.14064059642595667	2.6609331622708066
>100	0.038937745017131285	2.0175912172671104
>500	0.0036449761296900043	0.7591577973588883
>1k	0.0019881687980127295	1.4745471508316972
>5k	0.0	0.0
>10k+	1.6568073316772748E-4	1.9976679829477988
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40895	1.9743277050872432	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	4879	0.23554823017778848	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4134	0.19958114030641066	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	3895	0.1880427047637807	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	2948	0.1423234643500964	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	2460	0.11876381353501939	TruSeq Adapter, Index 13 (95% over 21bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2425	0.11707408448065935	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11514296556139075	0.0
2	0.0	0.0	0.0	0.32602115154552275	0.0
3	0.0	0.0	0.0	0.6050678353798367	0.0
4	0.0	0.0	0.0	0.8978254635409576	0.0
5	0.0	0.0	0.0	1.6628382234092167	0.0
6	0.0	0.0	0.0	3.149413567462191	0.0
7	0.0	0.0	0.0	4.02618983478312	0.0
8	0.0	0.0	0.0	5.560801761952902	0.0
9	0.0	0.0	0.0	6.501015285771805	0.0
10	0.0	0.0	0.0	7.553861320557051	0.0
11	0.0	0.0	0.0	8.310087489342637	0.0
12	0.0	0.0	0.0	8.832310323085851	0.0
13	4.82779729817152E-5	0.0	0.0	9.14862760206205	0.0
14	4.82779729817152E-5	0.0	0.0	9.349946749395802	0.0
15	4.82779729817152E-5	0.0	0.0	9.506898439559357	0.0
16	4.82779729817152E-5	0.0	0.0	9.724632097706893	0.0
17	4.82779729817152E-5	0.0	0.0	9.986009043429899	0.0
18	4.82779729817152E-5	0.0	0.0	10.319996060517404	0.0
19	4.82779729817152E-5	0.0	0.0	10.514652847579681	0.0
20	4.82779729817152E-5	0.0	0.0	10.716599608562195	0.0
21	4.82779729817152E-5	0.0	0.0	10.938919674142994	0.0
22	4.82779729817152E-5	0.0	0.0	11.200344897838981	0.0
23	4.82779729817152E-5	0.0	0.0	11.479198469781368	0.0
24	4.82779729817152E-5	0.0	0.0	11.704849715497906	0.0
25	4.82779729817152E-5	0.0	0.0	11.89578909864059	0.0
26	4.82779729817152E-5	0.0	0.0	12.081417904755284	0.0
27	4.82779729817152E-5	0.0	0.0	12.293165094253087	0.0
28	4.82779729817152E-5	0.0	0.0	12.516402441320539	0.0
29	4.82779729817152E-5	0.0	0.0	12.747219430146117	0.0
30	4.82779729817152E-5	0.0	0.0	13.03099735533264	0.0
31	4.82779729817152E-5	0.0	0.0	13.271325104835618	0.0
32	4.82779729817152E-5	0.0	0.0	13.513487417311902	0.0
33	4.82779729817152E-5	0.0	0.0	13.743435402623811	0.0
34	4.82779729817152E-5	0.0	0.0	13.975217950909025	0.0
35	4.82779729817152E-5	0.0	0.0	14.239877798794788	0.0
36	4.82779729817152E-5	0.0	0.0	14.471998292890875	0.0
37	4.82779729817152E-5	0.0	0.0	14.715657222529591	0.0
38	4.82779729817152E-5	0.0	0.0	14.977227280144525	0.0
39	4.82779729817152E-5	0.0	0.0	15.330815154262607	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17795	0.0	43.558582	1
TTGCACG	80	0.0	42.187504	1
TAAGACG	65	0.0	41.53846	1
TACGGGA	810	0.0	41.11111	4
TTTAGCG	140	0.0	40.17857	1
ACGGGAT	1160	0.0	38.98707	5
TAGGGAC	2650	0.0	38.88679	5
GCGCGAC	650	0.0	38.76923	9
CGGTTTT	1645	0.0	38.708206	1
TAGGGAT	3325	0.0	38.503757	5
CGAGGGA	1655	0.0	38.338367	4
ATAGGGA	2960	0.0	38.2348	4
AAGGGAT	3575	0.0	37.888115	5
CGATCGA	60	1.5643309E-10	37.500004	10
CGGTCTA	270	0.0	37.5	31
CGACGGT	290	0.0	37.24138	28
CTAGGGA	1520	0.0	37.154606	4
AGGGATC	2275	0.0	36.989014	6
TAAGGGA	2875	0.0	36.93913	4
GCGTTAG	195	0.0	36.923077	1
>>END_MODULE