FastQCFastQC Report
Thu 26 May 2016
SRR1547911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547911_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4349607
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331810.7628505287949003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT189850.042.4282341
TCACGAC5250.041.99999625
CGTTAGG6950.039.4964032
TACGGGA13150.038.66924
CACGACC6950.038.5251827
CGAGGGA24550.038.126274
AGGGCGA34450.037.9462976
TAGGGCG12000.037.5000045
GGGCGAT66550.037.3929377
CACGACG5600.037.3660726
CGAGGGT13550.037.3616264
CGGTAGT7500.036.912
ACGGTCT5800.036.85344730
ATAGGGT29050.036.7900164
TACGGGT10400.036.7788434
CGCATCG1850.036.48648521
TAGGGTC22500.036.45
GCGCGAC7300.036.3698659
TAGGGTA34150.036.368965
CTCACGA6250.036.35999724
Produced by FastQC (version 0.11.5)