##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547911_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4349607
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48124278814155	31.0	31.0	33.0	30.0	34.0
2	31.894062613013084	31.0	31.0	34.0	30.0	34.0
3	31.89546572828304	33.0	31.0	34.0	30.0	34.0
4	35.72867525732784	37.0	35.0	37.0	35.0	37.0
5	35.6942601940819	37.0	35.0	37.0	33.0	37.0
6	35.747811698850036	37.0	35.0	37.0	33.0	37.0
7	36.02934793879079	37.0	35.0	37.0	35.0	37.0
8	36.04976104737738	37.0	35.0	37.0	35.0	37.0
9	37.8099600722548	39.0	38.0	39.0	35.0	39.0
10	37.230616007377215	39.0	37.0	39.0	34.0	39.0
11	37.08586040072126	39.0	37.0	39.0	33.0	39.0
12	36.71266507525852	39.0	35.0	39.0	33.0	39.0
13	36.60701001262873	39.0	35.0	39.0	33.0	39.0
14	37.6914475261788	40.0	36.0	41.0	33.0	41.0
15	37.82266926644177	40.0	36.0	41.0	33.0	41.0
16	37.881690920582024	40.0	36.0	41.0	33.0	41.0
17	37.807241895647124	40.0	36.0	41.0	33.0	41.0
18	37.67352682667652	39.0	36.0	41.0	33.0	41.0
19	37.580383009315554	39.0	36.0	41.0	33.0	41.0
20	37.43050257184155	39.0	35.0	41.0	32.0	41.0
21	37.34152671724135	39.0	35.0	41.0	32.0	41.0
22	37.24093739963174	39.0	35.0	41.0	32.0	41.0
23	37.130210384524396	39.0	35.0	41.0	32.0	41.0
24	37.15769838516445	39.0	35.0	41.0	32.0	41.0
25	37.13243771218871	39.0	35.0	41.0	32.0	41.0
26	37.04857703236178	39.0	35.0	41.0	32.0	41.0
27	36.982035388484526	39.0	35.0	41.0	32.0	41.0
28	36.89325518374419	39.0	35.0	41.0	31.0	41.0
29	36.8782853255478	39.0	35.0	41.0	31.0	41.0
30	36.77476194975776	39.0	35.0	41.0	31.0	41.0
31	36.60970910705266	39.0	35.0	41.0	31.0	41.0
32	36.45937713453193	39.0	35.0	41.0	30.0	41.0
33	36.322611215220135	39.0	35.0	41.0	30.0	41.0
34	36.13133370440134	39.0	35.0	41.0	30.0	41.0
35	35.993531139709866	39.0	35.0	41.0	29.0	41.0
36	35.88302989212588	38.0	35.0	41.0	29.0	41.0
37	35.79048911775248	38.0	35.0	41.0	28.0	41.0
38	35.6749244242066	38.0	35.0	41.0	27.0	41.0
39	35.580800748205526	38.0	35.0	40.0	27.0	41.0
40	35.43844673783172	38.0	35.0	40.0	26.0	41.0
41	35.362596436873496	38.0	35.0	40.0	26.0	41.0
42	35.302993810705196	38.0	34.0	40.0	26.0	41.0
43	35.25258465879791	38.0	34.0	40.0	26.0	41.0
44	35.07292245023516	38.0	34.0	40.0	25.0	41.0
45	34.94852822335443	38.0	34.0	40.0	24.0	41.0
46	34.90706884553018	38.0	34.0	40.0	24.0	41.0
47	34.839221566454164	38.0	34.0	40.0	24.0	41.0
48	34.72996893742354	37.0	34.0	40.0	24.0	41.0
49	34.586985444891916	37.0	34.0	40.0	24.0	41.0
50	34.52750282956598	37.0	34.0	40.0	24.0	41.0
51	34.2149116000595	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	9.0
12	13.0
13	27.0
14	41.0
15	125.0
16	270.0
17	726.0
18	1630.0
19	3102.0
20	5396.0
21	8938.0
22	13623.0
23	20517.0
24	31228.0
25	47737.0
26	64624.0
27	73808.0
28	76629.0
29	82764.0
30	95517.0
31	113973.0
32	141139.0
33	184220.0
34	303332.0
35	419174.0
36	316433.0
37	424842.0
38	689250.0
39	1230351.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.05009096224096	24.72301520574158	28.5605573101202	14.666336521897266
2	33.16136377378462	25.44390792087653	28.297958873066005	13.096769432272847
3	29.43321086249861	24.999545935989158	31.87901803542251	13.688225166089719
4	25.542698455285727	27.303961024524742	31.917458289909874	15.235882230279655
5	23.350684326193146	31.559265009459477	30.436611859416264	14.653438804931113
6	21.612918132603703	40.05159546598118	27.601666081556335	10.733820319858783
7	87.53381627351621	3.2213714940223332	7.590823722694946	1.6539885097665143
8	88.04402328762116	2.4988464475066365	7.511483221357699	1.945647043514506
9	83.11206046891132	4.65504124855418	9.404918651271252	2.827979631263238
10	37.33790202195279	26.84477011371372	24.075508430991583	11.74181943334191
11	32.709253962484425	23.587855178640275	26.181951610800702	17.520939248074598
12	29.790116670310674	22.420577307329147	29.167738602590994	18.62156741976919
13	24.800539451035462	26.474759673690063	29.645069083252807	19.07963179202167
14	20.080848683570725	29.568395489523535	30.734822709270055	19.615933117635688
15	19.609380801529884	26.12587757928475	36.6484834147085	17.616258204476864
16	23.051599834191915	23.97237267642801	34.702215625457654	18.27381186392242
17	23.07769414570098	24.014353480670785	29.119872209144415	23.788080164483826
18	23.810541963906164	25.230003538250696	31.4490711459679	19.510383351875237
19	25.60953207956489	26.605805995806058	28.118425411767085	19.666236512861968
20	26.98052950530933	26.241773107317513	27.65357881758053	19.12411856979263
21	24.957886999905966	26.294858363065902	30.086902104029168	18.660352532998957
22	24.054380085373232	23.073739765454672	30.411115303060715	22.460764846111385
23	21.935062179180786	26.519499347872117	29.643252827209448	21.90218564573765
24	21.827903072622423	26.002877961158326	32.2995387859179	19.869680180301348
25	21.874137134688258	26.50802704704126	30.520021693913957	21.097814124356525
26	20.852780492582433	28.7350328431971	28.259564599744298	22.152622064476173
27	20.28939626039778	27.946961645040574	31.00470916108053	20.758932933481116
28	18.969690824941196	27.524072864513965	32.86377366966717	20.64246264087767
29	20.02900491929501	26.080540149949176	32.46739303114051	21.4230618996153
30	21.729204500544533	26.00122263919476	31.673321290866046	20.59625156939466
31	24.50363906440283	25.69786189878764	28.521312385233884	21.277186651575647
32	24.65477915590995	26.39144180152368	28.558143298923326	20.395635743643044
33	23.851097352013642	25.98735931774986	28.31561564067742	21.84592768955908
34	20.848458263010887	26.73489811838173	30.59021194328591	21.826431675321473
35	21.490056457974248	26.908109169403122	29.321499620540433	22.2803347520822
36	24.376777028361413	26.48402487856949	28.233516269400894	20.905681823668207
37	21.40119785534647	28.626011499429723	28.796394708763344	21.176395936460466
38	21.690925180136965	28.738642364700993	27.09470073963004	22.475731715532003
39	21.420670879001253	27.445284137164577	27.904061217484706	23.229983766349466
40	22.817601682174963	26.293685843341706	28.56963859033701	22.319073884146317
41	20.036982651536103	25.41314652105351	29.74928539520927	24.800585432201117
42	21.83606472952614	26.720483022948972	28.13465676324321	23.30879548428168
43	21.74311380315509	26.09867971979997	28.786600720478884	23.37160575656605
44	21.684119967620063	27.39153215451419	28.678981802263976	22.245366075601773
45	21.001667506972467	28.474549539763018	27.312168662594118	23.211614290670397
46	22.162691939754556	27.833411156456204	27.856125852289644	22.147771051499596
47	21.52182944344167	26.931214705144622	29.16670402636376	22.38025182504994
48	21.98725080219891	26.27628197214139	29.759493213984623	21.976974011675075
49	21.677038868109232	25.421836961362253	29.417117454519452	23.484006716009056
50	20.39168136339674	26.990185550096825	30.006412073550553	22.611721012955883
51	20.07388253697403	27.707790611887468	28.549682764442856	23.668644086695647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2373.0
1	3813.0
2	5253.0
3	30822.0
4	56391.0
5	41630.0
6	26869.0
7	24626.5
8	22384.0
9	22771.5
10	23159.0
11	22889.5
12	22620.0
13	22273.0
14	21926.0
15	20824.5
16	19723.0
17	18920.0
18	18117.0
19	17572.5
20	17028.0
21	17671.5
22	18315.0
23	19925.5
24	21536.0
25	25583.0
26	33019.0
27	36408.0
28	44080.5
29	51753.0
30	61838.0
31	71923.0
32	82893.5
33	93864.0
34	108316.5
35	122769.0
36	135019.5
37	147270.0
38	162886.0
39	178502.0
40	206498.5
41	234495.0
42	261217.0
43	287939.0
44	308087.0
45	328235.0
46	345922.0
47	363609.0
48	363282.5
49	362956.0
50	347932.0
51	332908.0
52	312782.0
53	292656.0
54	266278.0
55	239900.0
56	225449.5
57	210999.0
58	196862.0
59	182725.0
60	167073.5
61	151422.0
62	137535.5
63	123649.0
64	104053.5
65	84458.0
66	69353.0
67	54248.0
68	45506.5
69	36765.0
70	29658.0
71	22551.0
72	18178.5
73	13806.0
74	11480.0
75	6880.5
76	4607.0
77	3728.5
78	2850.0
79	1896.5
80	943.0
81	699.0
82	455.0
83	321.5
84	188.0
85	165.0
86	142.0
87	94.5
88	47.0
89	42.0
90	37.0
91	27.0
92	17.0
93	22.5
94	28.0
95	15.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4349607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.058288759503185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1772858796409	14.708669999689238
2	7.839376547958879	2.9881020389095516
3	2.713757156414508	1.5515870253034785
4	1.4412087043059896	1.0986788659749198
5	0.9260380357029819	0.8824350143355274
6	0.6568003647278655	0.7510494604998402
7	0.5059634222817135	0.674995790251394
8	0.38223607870383386	0.5827812449790281
9	0.3168248027757365	0.5434324719725369
>10	4.7149355942686615	23.091442821836544
>50	2.7795982630361418	38.15521358639416
>100	0.5389481428096388	12.809088432449695
>500	0.004561390750935178	0.5950398601034345
>1k	0.0023423357910189297	0.7878885994115323
>5k	0.0	0.0
>10k+	1.2328083110625944E-4	0.7795947878891292
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33181	0.7628505287949003	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.897174848210425E-5	0.0	0.0	0.04402696611440988	0.0
2	6.897174848210425E-5	0.0	0.0	0.1363341561662927	0.0
3	6.897174848210425E-5	0.0	0.0	0.2357454363118323	0.0
4	6.897174848210425E-5	0.0	0.0	0.37985040947377546	0.0
5	6.897174848210425E-5	0.0	0.0	0.7052821093951707	0.0
6	6.897174848210425E-5	0.0	0.0	1.2340655144246366	0.0
7	6.897174848210425E-5	0.0	0.0	1.5273563795533711	0.0
8	6.897174848210425E-5	0.0	0.0	2.1322845949070803	0.0
9	6.897174848210425E-5	0.0	0.0	2.4511639787226756	0.0
10	6.897174848210425E-5	0.0	0.0	2.9426106772404954	0.0
11	6.897174848210425E-5	0.0	0.0	3.3704654236578153	0.0
12	6.897174848210425E-5	0.0	0.0	3.6853904272271034	0.0
13	6.897174848210425E-5	0.0	0.0	3.833886601709074	0.0
14	6.897174848210425E-5	0.0	0.0	3.913709905285696	0.0
15	6.897174848210425E-5	0.0	0.0	4.004913547361865	0.0
16	6.897174848210425E-5	0.0	0.0	4.194792770933098	0.0
17	6.897174848210425E-5	0.0	0.0	4.398627278280544	0.0
18	6.897174848210425E-5	0.0	0.0	4.66945634398694	0.0
19	6.897174848210425E-5	0.0	0.0	4.811285249448973	0.0
20	6.897174848210425E-5	0.0	0.0	4.9725182068173055	0.0
21	6.897174848210425E-5	0.0	0.0	5.170145256801361	0.0
22	6.897174848210425E-5	0.0	0.0	5.38959036988859	0.0
23	6.897174848210425E-5	0.0	0.0	5.613679580706947	0.0
24	6.897174848210425E-5	0.0	0.0	5.7893966052565204	0.0
25	6.897174848210425E-5	0.0	0.0	5.949640967563276	0.0
26	6.897174848210425E-5	0.0	0.0	6.105379175635868	0.0
27	6.897174848210425E-5	0.0	0.0	6.277118829356307	0.0
28	6.897174848210425E-5	0.0	0.0	6.447065217616212	0.0
29	6.897174848210425E-5	0.0	0.0	6.6420943317407755	0.0
30	6.897174848210425E-5	0.0	0.0	6.8753108039415975	0.0
31	6.897174848210425E-5	0.0	0.0	7.07052384272878	0.0
32	6.897174848210425E-5	0.0	0.0	7.2621733411777205	0.0
33	6.897174848210425E-5	0.0	0.0	7.454765453522582	0.0
34	6.897174848210425E-5	0.0	0.0	7.657933233968034	0.0
35	6.897174848210425E-5	0.0	0.0	7.888206911566953	0.0
36	6.897174848210425E-5	0.0	0.0	8.086891528361068	0.0
37	9.196233130947233E-5	0.0	0.0	8.306152716785677	0.0
38	9.196233130947233E-5	0.0	0.0	8.553324472762712	0.0
39	9.196233130947233E-5	0.0	0.0	8.93253114591732	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18985	0.0	42.428234	1
TCACGAC	525	0.0	41.999996	25
CGTTAGG	695	0.0	39.496403	2
TACGGGA	1315	0.0	38.6692	4
CACGACC	695	0.0	38.52518	27
CGAGGGA	2455	0.0	38.12627	4
AGGGCGA	3445	0.0	37.946297	6
TAGGGCG	1200	0.0	37.500004	5
GGGCGAT	6655	0.0	37.392937	7
CACGACG	560	0.0	37.36607	26
CGAGGGT	1355	0.0	37.361626	4
CGGTAGT	750	0.0	36.9	12
ACGGTCT	580	0.0	36.853447	30
ATAGGGT	2905	0.0	36.790016	4
TACGGGT	1040	0.0	36.778843	4
CGCATCG	185	0.0	36.486485	21
TAGGGTC	2250	0.0	36.4	5
GCGCGAC	730	0.0	36.369865	9
TAGGGTA	3415	0.0	36.36896	5
CTCACGA	625	0.0	36.359997	24
>>END_MODULE