FastQCFastQC Report
Thu 26 May 2016
SRR1547910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547910_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2152964
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177510.824491259491566No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37500.17417848138659078No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG207.0346624E-445.09
CGGTCTA2700.044.16666431
CGTTTTT66850.042.3074041
TACGTAG603.6379788E-1241.2500041
TCACGAC2900.041.1206925
TAGTACG1000.040.51
ACGGTCT2900.040.34482630
CCGCTCG950.040.26315719
TATACGA403.4600998E-739.37516
GCGTAAG1500.039.0000041
TACGGGA6650.038.9097754
TCGTAAG1450.038.7931021
TGCGTAG1450.038.7931021
ACCGCTC1050.038.5714318
CGTTAGG3450.038.478262
GGGCGAT28500.038.447377
CGCGTAA1000.038.2531
TAAGGGA25850.038.210834
ACGGGAT6550.038.1297725
AGGGCGA15000.037.86
Produced by FastQC (version 0.11.5)