##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547910_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2152964
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47143519352855	31.0	31.0	33.0	30.0	34.0
2	31.875723885768643	31.0	31.0	34.0	30.0	34.0
3	31.9160315732172	33.0	31.0	34.0	30.0	34.0
4	35.70067126064347	37.0	35.0	37.0	33.0	37.0
5	35.628681668620565	37.0	35.0	37.0	33.0	37.0
6	35.673148273728685	37.0	35.0	37.0	33.0	37.0
7	36.00211801033366	37.0	35.0	37.0	35.0	37.0
8	36.02574590192869	37.0	35.0	37.0	35.0	37.0
9	37.79195379021665	39.0	38.0	39.0	35.0	39.0
10	37.30811894671718	39.0	37.0	39.0	34.0	39.0
11	36.99073138241048	39.0	37.0	39.0	33.0	39.0
12	36.26438528465873	38.0	35.0	39.0	32.0	39.0
13	35.99709516740642	38.0	35.0	39.0	32.0	39.0
14	36.992741169847704	39.0	35.0	41.0	32.0	41.0
15	37.234931935694235	39.0	35.0	41.0	32.0	41.0
16	37.37227190050554	39.0	35.0	41.0	33.0	41.0
17	37.29309407867479	39.0	35.0	41.0	32.0	41.0
18	37.19837860735247	39.0	35.0	41.0	32.0	41.0
19	37.11127682580852	38.0	35.0	41.0	32.0	41.0
20	36.95679723395282	38.0	35.0	41.0	32.0	41.0
21	36.819259866862616	38.0	35.0	41.0	32.0	41.0
22	36.71529807279639	38.0	35.0	41.0	31.0	41.0
23	36.65487950564896	38.0	35.0	41.0	31.0	41.0
24	36.659248366438085	38.0	35.0	41.0	32.0	41.0
25	36.604020782511924	38.0	35.0	41.0	31.0	41.0
26	36.486619376821906	38.0	35.0	40.0	31.0	41.0
27	36.38653131218172	38.0	35.0	40.0	31.0	41.0
28	36.32053903362991	38.0	35.0	40.0	31.0	41.0
29	36.33688394232323	38.0	35.0	40.0	31.0	41.0
30	36.25572838189584	38.0	35.0	40.0	31.0	41.0
31	36.08563635992056	38.0	35.0	40.0	30.0	41.0
32	35.90397935125715	38.0	35.0	40.0	30.0	41.0
33	35.79584006049335	38.0	35.0	40.0	30.0	41.0
34	35.676328540560824	38.0	35.0	40.0	29.0	41.0
35	35.582196915508106	38.0	35.0	40.0	29.0	41.0
36	35.482429803749625	38.0	35.0	40.0	29.0	41.0
37	35.376140056220166	37.0	34.0	40.0	28.0	41.0
38	35.29127890666077	37.0	34.0	40.0	27.0	41.0
39	35.2107229846853	37.0	34.0	40.0	27.0	41.0
40	35.06412972999084	37.0	34.0	40.0	26.0	41.0
41	35.016596654658414	37.0	34.0	40.0	26.0	41.0
42	34.94314628577162	37.0	34.0	40.0	26.0	41.0
43	34.86092057275459	37.0	34.0	40.0	26.0	41.0
44	34.677238913423544	36.0	34.0	40.0	25.0	41.0
45	34.535849182801016	36.0	34.0	40.0	24.0	41.0
46	34.49908637580563	36.0	34.0	40.0	24.0	41.0
47	34.42469126283579	36.0	34.0	40.0	24.0	41.0
48	34.332908028188115	36.0	34.0	40.0	24.0	41.0
49	34.213414158341706	36.0	34.0	40.0	24.0	41.0
50	34.118047956212926	35.0	33.0	40.0	24.0	41.0
51	33.793385305095676	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	4.0
12	6.0
13	10.0
14	31.0
15	68.0
16	196.0
17	418.0
18	916.0
19	1761.0
20	3203.0
21	5137.0
22	7704.0
23	11380.0
24	16876.0
25	24029.0
26	30940.0
27	35337.0
28	39318.0
29	44642.0
30	52579.0
31	63392.0
32	78753.0
33	104004.0
34	189410.0
35	294861.0
36	153812.0
37	185202.0
38	286026.0
39	522869.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.190568908723044	23.820881352405333	29.412149947700005	13.576399791171612
2	30.21369609524358	26.338712584139817	30.074260414944238	13.373330905672365
3	29.401652791221778	25.25522953472515	31.505589503586684	13.83752817046639
4	25.709905042536707	27.93952894707018	31.29141964287373	15.059146367519382
5	23.00767685850762	32.10792191601903	30.24086793833989	14.643533287133458
6	21.716851744850356	41.4418448241587	26.637231277438918	10.204072153552033
7	88.04671141737623	3.796765761062424	6.418686053273534	1.7378367682878118
8	88.5766320291468	3.0123587760872916	6.111946135652988	2.299063059112925
9	84.24437194490943	5.214392809169127	7.792605914450962	2.748629331470475
10	46.929349492141995	28.642606193136533	13.082011589603916	11.34603272511756
11	38.66125954730316	22.859834163506683	23.24808961041615	15.230816678774007
12	35.89929975605723	22.598473546236722	25.61752077600926	15.884705921696787
13	23.001127747607484	34.10990615727899	26.44744640412009	16.441519690993438
14	16.34760265382979	37.68219069153037	29.100904613360928	16.869302041278907
15	15.41846496272116	27.05219409149433	41.85843330404038	15.670907641744126
16	19.417045524216846	23.343539418216004	39.89388582437979	17.345529233187364
17	19.07788518526088	24.363157024455585	27.791314671308946	28.767643118974583
18	22.30613238307747	27.00481754455718	31.55719278167215	19.1318572906932
19	25.999320007208667	28.423838020514975	26.286505487318877	19.290336484957482
20	28.180824203284406	27.286847341618348	25.16433159123887	19.367996863858384
21	22.932803335308904	28.9872473483068	28.799088140814245	19.280861175570053
22	22.275198284783208	25.263590101831706	28.592024762141865	23.869186851243214
23	19.44375289136279	30.288244485277044	26.899381503824493	23.368621119535675
24	20.515020223282878	26.682889263359723	34.16211325409993	18.63997725925747
25	19.425498986513475	27.366179833940556	32.024780720903834	21.183540458642135
26	17.970992547947855	33.48918049721222	26.975973588039558	21.563853366800373
27	18.028959146553312	32.71592093504582	29.960742492675212	19.29437742572565
28	16.01921815692227	29.943324644536556	35.08967172697732	18.94778547156385
29	17.123184595748	27.630420202102773	34.16791920347948	21.07847599866974
30	19.70162993900502	29.928089833364606	30.70427559401829	19.66600463361208
31	26.33077004538859	28.122904052273984	25.420397182674677	20.125928719662753
32	26.49914257739563	28.94632701707971	25.74854944160701	18.80598096391765
33	24.807521166169057	30.237709501877415	25.186208408501027	19.768560923452505
34	19.25113471474674	29.767892078083978	28.025224759912383	22.955748447256898
35	18.844764705773066	29.63347273804857	29.61280355825736	21.908958997921005
36	25.73925992259973	27.526098903650965	27.12246930278444	19.612171870964865
37	19.80516162834121	31.578279989818686	28.885991591127397	19.73056679071271
38	19.744175936058383	32.62720602852626	25.53437958089406	22.0942384545213
39	19.209006746048704	31.34985071742955	27.958572461035114	21.48257007548663
40	22.268277593122786	27.966329209406194	26.790043865108753	22.975349332362267
41	18.251814707538074	26.648936071388096	28.346642117564436	26.752607103509398
42	21.96214149423771	27.543423856599553	26.14609440752377	24.34834024163897
43	21.622423784141304	27.965678943075684	26.65079397518955	23.761103297593458
44	19.76085062267646	30.86526295841454	27.42684039305813	21.947046025850874
45	18.765292870665743	34.055423128301264	24.664648363837017	22.514635637195976
46	22.19725922031209	31.107533614124527	26.19449280155172	20.50071436401166
47	21.076200066512957	28.970293976118505	27.12428075899086	22.829225198377678
48	22.18374297015649	26.31311995927475	29.67318543180471	21.829951638764047
49	21.037648562632725	25.486677900791655	29.873049433246447	23.602624103329177
50	18.91573663098872	30.417461694668376	28.87646983414493	21.79033184019798
51	18.012748935885597	32.097099626375545	26.241126186968287	23.649025250770567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2227.0
1	2835.0
2	3443.0
3	13887.5
4	24332.0
5	17210.0
6	10088.0
7	9825.0
8	9562.0
9	11265.0
10	12968.0
11	14206.5
12	15445.0
13	15494.0
14	15543.0
15	14416.0
16	13289.0
17	12432.0
18	11575.0
19	11025.0
20	10475.0
21	10683.0
22	10891.0
23	11554.0
24	12217.0
25	13752.0
26	17681.0
27	20075.0
28	24008.0
29	27941.0
30	33661.0
31	39381.0
32	41545.0
33	43709.0
34	52150.0
35	60591.0
36	63103.0
37	65615.0
38	76525.0
39	87435.0
40	109641.0
41	131847.0
42	157224.0
43	182601.0
44	186389.0
45	190177.0
46	192602.5
47	195028.0
48	191766.5
49	188505.0
50	173903.5
51	159302.0
52	146536.5
53	133771.0
54	117616.0
55	101461.0
56	94150.0
57	86839.0
58	81035.0
59	75231.0
60	68290.5
61	61350.0
62	54188.0
63	47026.0
64	40043.0
65	33060.0
66	27250.0
67	21440.0
68	17225.5
69	13011.0
70	10672.5
71	8334.0
72	7032.5
73	5731.0
74	4174.0
75	2182.0
76	1747.0
77	1276.0
78	805.0
79	640.0
80	475.0
81	346.5
82	218.0
83	172.0
84	126.0
85	115.0
86	104.0
87	67.5
88	31.0
89	26.0
90	21.0
91	16.5
92	12.0
93	7.5
94	3.0
95	1.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2152964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.88736721400402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45923975427115	15.40460345110769
2	8.863101623515208	3.525275132837643
3	3.2365141895875804	1.930972385449885
4	1.6741843927704454	1.3318047921192075
5	0.9297196164469078	0.9244837709171314
6	0.6614590873405073	0.7892807860188376
7	0.45682132860782537	0.6359481459249117
8	0.36134817894386245	0.5749011141414582
9	0.2892920958465533	0.5177932327988327
>10	3.0027846652368706	14.572293553432056
>50	1.9479777575338098	28.778774465962975
>100	1.1113960525482298	29.083814457583895
>500	0.004265485858422464	0.5326807737072723
>1k	0.0016588000560531805	0.5608158194387809
>5k	0.0	0.0
>10k+	2.369714365790258E-4	0.836558118559479
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17751	0.824491259491566	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3750	0.17417848138659078	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6447595036424204E-5	0.0	0.0	0.06001029278706007	0.0
2	4.6447595036424204E-5	0.0	0.0	0.2193719913570315	0.0
3	4.6447595036424204E-5	0.0	0.0	0.3340511035019629	0.0
4	4.6447595036424204E-5	0.0	0.0	0.5688436964110872	0.0
5	4.6447595036424204E-5	0.0	0.0	1.022776042702061	0.0
6	4.6447595036424204E-5	0.0	0.0	1.62640898779543	0.0
7	4.6447595036424204E-5	0.0	0.0	1.9981755384669693	0.0
8	4.6447595036424204E-5	0.0	0.0	2.76363190466724	0.0
9	4.6447595036424204E-5	0.0	0.0	3.092434429930087	0.0
10	4.6447595036424204E-5	0.0	0.0	3.5843609089608557	0.0
11	4.6447595036424204E-5	0.0	0.0	4.172805490477314	0.0
12	4.6447595036424204E-5	0.0	0.0	4.601795478233728	0.0
13	4.6447595036424204E-5	0.0	0.0	4.780014900388488	0.0
14	4.6447595036424204E-5	0.0	0.0	4.851265511174362	0.0
15	4.6447595036424204E-5	0.0	0.0	4.957491161022665	0.0
16	4.6447595036424204E-5	0.0	0.0	5.198786417236889	0.0
17	4.6447595036424204E-5	0.0	0.0	5.4895483621649035	0.0
18	4.6447595036424204E-5	0.0	0.0	5.854719354341271	0.0
19	9.289519007284841E-5	0.0	0.0	6.046733712221848	0.0
20	9.289519007284841E-5	0.0	0.0	6.238608727317317	0.0
21	9.289519007284841E-5	0.0	0.0	6.491841015455901	0.0
22	9.289519007284841E-5	0.0	0.0	6.758961134510377	0.0
23	9.289519007284841E-5	0.0	0.0	7.041873435877237	0.0
24	9.289519007284841E-5	0.0	0.0	7.258458571532083	0.0
25	9.289519007284841E-5	0.0	0.0	7.437792735967717	0.0
26	9.289519007284841E-5	0.0	0.0	7.608998571271977	0.0
27	9.289519007284841E-5	0.0	0.0	7.784616928104697	0.0
28	9.289519007284841E-5	0.0	0.0	7.960560418102672	0.0
29	9.289519007284841E-5	0.0	0.0	8.166044578543811	0.0
30	9.289519007284841E-5	0.0	0.0	8.421645694029255	0.0
31	9.289519007284841E-5	0.0	0.0	8.644919283369346	0.0
32	9.289519007284841E-5	0.0	0.0	8.852214900016907	0.0
33	9.289519007284841E-5	0.0	0.0	9.062947638697164	0.0
34	9.289519007284841E-5	0.0	0.0	9.283480819930105	0.0
35	9.289519007284841E-5	0.0	0.0	9.550508043794508	0.0
36	9.289519007284841E-5	0.0	0.0	9.77819415466306	0.0
37	9.289519007284841E-5	0.0	0.0	10.008713568828833	0.0
38	9.289519007284841E-5	0.0	0.0	10.244063532878394	0.0
39	9.289519007284841E-5	0.0	0.0	10.576628313339192	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	20	7.0346624E-4	45.0	9
CGGTCTA	270	0.0	44.166664	31
CGTTTTT	6685	0.0	42.307404	1
TACGTAG	60	3.6379788E-12	41.250004	1
TCACGAC	290	0.0	41.12069	25
TAGTACG	100	0.0	40.5	1
ACGGTCT	290	0.0	40.344826	30
CCGCTCG	95	0.0	40.263157	19
TATACGA	40	3.4600998E-7	39.375	16
GCGTAAG	150	0.0	39.000004	1
TACGGGA	665	0.0	38.909775	4
TCGTAAG	145	0.0	38.793102	1
TGCGTAG	145	0.0	38.793102	1
ACCGCTC	105	0.0	38.57143	18
CGTTAGG	345	0.0	38.47826	2
GGGCGAT	2850	0.0	38.44737	7
CGCGTAA	100	0.0	38.25	31
TAAGGGA	2585	0.0	38.21083	4
ACGGGAT	655	0.0	38.129772	5
AGGGCGA	1500	0.0	37.8	6
>>END_MODULE