FastQCFastQC Report
Thu 26 May 2016
SRR1547909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547909_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences918543
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC40200.4376496255482868No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37900.41260997035522556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG351.2115743E-745.0000041
TCGATAG351.2115743E-745.0000041
CGCCTAA850.045.00000430
TAGGACG351.2115743E-745.0000041
CACCGGG600.045.0000043
AGTCGGT302.165023E-645.00000412
ATTTACG253.8901915E-545.01
ACAACGA207.032547E-445.014
CTTAACG207.032547E-445.01
CGGAATA207.032547E-445.010
CGGGTAT453.8380676E-1045.06
AGGTACG253.8901915E-545.01
GTCGATG253.8901915E-545.01
CCCTCCG207.032547E-445.044
CGAAACG207.032547E-445.035
CGACGCA207.032547E-445.014
ACGTTAG551.8189894E-1245.01
TTTGTCG207.032547E-445.01
CGTGATT207.032547E-445.013
GCTATTG502.1827873E-1145.01