##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547909_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	918543
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.573742328883895	31.0	31.0	33.0	30.0	34.0
2	31.98657874481652	31.0	31.0	34.0	30.0	34.0
3	32.0301379467265	33.0	31.0	34.0	30.0	34.0
4	35.792536658599545	37.0	35.0	37.0	35.0	37.0
5	35.72163633058006	37.0	35.0	37.0	33.0	37.0
6	35.776742079576024	37.0	35.0	37.0	35.0	37.0
7	36.05565226668757	37.0	35.0	37.0	35.0	37.0
8	36.0833874951962	37.0	35.0	37.0	35.0	37.0
9	37.88411756444717	39.0	38.0	39.0	35.0	39.0
10	37.47333331155972	39.0	37.0	39.0	35.0	39.0
11	37.1149527022687	39.0	37.0	39.0	33.0	39.0
12	35.879746511594995	37.0	35.0	39.0	32.0	39.0
13	35.35174727802618	37.0	35.0	39.0	31.0	39.0
14	36.247257885586194	38.0	35.0	41.0	31.0	41.0
15	36.62432569841586	38.0	35.0	41.0	32.0	41.0
16	36.83954371216154	38.0	35.0	41.0	33.0	41.0
17	36.80065821632738	38.0	35.0	41.0	33.0	41.0
18	36.728723641680354	37.0	35.0	40.0	32.0	41.0
19	36.61859923814127	37.0	35.0	40.0	32.0	41.0
20	36.435713951333796	36.0	35.0	40.0	32.0	41.0
21	36.277063784711224	36.0	35.0	40.0	32.0	41.0
22	36.1629537212738	36.0	35.0	40.0	31.0	41.0
23	36.154076619167526	36.0	35.0	40.0	31.0	41.0
24	36.14134232148087	35.0	35.0	40.0	32.0	41.0
25	36.07301890058495	35.0	35.0	40.0	31.0	41.0
26	35.94262870654939	35.0	35.0	40.0	31.0	41.0
27	35.842747699345594	35.0	35.0	40.0	31.0	41.0
28	35.852838680388395	36.0	35.0	40.0	31.0	41.0
29	35.92205264206466	36.0	35.0	40.0	31.0	41.0
30	35.861664614503624	36.0	35.0	40.0	31.0	41.0
31	35.617197017450465	35.0	35.0	40.0	31.0	41.0
32	35.38119500121388	35.0	34.0	40.0	30.0	41.0
33	35.2782047220435	35.0	34.0	40.0	30.0	41.0
34	35.163781118575834	35.0	34.0	40.0	29.0	41.0
35	35.078935879975134	35.0	34.0	40.0	29.0	41.0
36	34.923681308332874	35.0	34.0	40.0	29.0	41.0
37	34.75035028300254	35.0	34.0	40.0	27.0	41.0
38	34.68837822508037	35.0	34.0	40.0	27.0	41.0
39	34.66776950017582	35.0	34.0	40.0	27.0	41.0
40	34.44255413192415	35.0	34.0	40.0	25.0	41.0
41	34.455758739656176	35.0	34.0	40.0	26.0	41.0
42	34.364579556972295	35.0	34.0	40.0	25.0	41.0
43	34.25526295448335	35.0	34.0	40.0	26.0	41.0
44	34.0763774804228	35.0	34.0	40.0	24.0	41.0
45	33.90676865427095	35.0	34.0	40.0	23.0	41.0
46	33.90519768807775	35.0	34.0	39.0	23.0	41.0
47	33.84929502483825	35.0	33.0	39.0	23.0	41.0
48	33.804459889194085	35.0	33.0	39.0	23.0	41.0
49	33.748524565534765	35.0	34.0	39.0	24.0	41.0
50	33.63017953432774	35.0	33.0	39.0	24.0	41.0
51	33.2774535323877	35.0	33.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	5.0
14	13.0
15	25.0
16	73.0
17	176.0
18	386.0
19	815.0
20	1353.0
21	2179.0
22	3338.0
23	5248.0
24	7614.0
25	10933.0
26	14041.0
27	15716.0
28	17133.0
29	19551.0
30	23019.0
31	27950.0
32	35617.0
33	48887.0
34	102363.0
35	183762.0
36	53842.0
37	64615.0
38	99778.0
39	180070.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.8731948313797	24.545067568965198	30.09810101432377	13.483636585331334
2	29.67155593151328	26.36969635607696	31.26266271693323	12.69608499547653
3	28.448967549695553	25.344376909954136	32.72595839280251	13.4806971475478
4	25.36658599542972	28.793099506501058	30.662473068762157	15.177841429307065
5	23.37821963696855	32.654105469205035	29.8803648822102	14.087310011616221
6	21.287408428347938	41.51531283783122	26.7031592424089	10.494119491411942
7	87.93328129439776	4.705060078842253	6.0006989329840845	1.3609596937759039
8	89.01564760713434	3.609629598178855	5.096114172118235	2.278608622568568
9	86.07087528836429	5.310039921919823	6.5578856950627245	2.061199094653163
10	57.14539221353818	23.574291023936823	10.552799379016552	8.727517383508447
11	51.81129244901981	18.75775004545242	17.511210689102196	11.919746816425578
12	47.168396035895974	20.184030578862394	20.138305991118543	12.509267394123084
13	21.20064057970068	46.29908452843253	20.240533105145868	12.259741786720927
14	13.984538557258617	48.26589500981446	24.559547021750753	13.190019411176179
15	11.666955167041717	24.399075492383044	51.20685694627252	12.727112394302717
16	13.954382102960885	18.728355667617087	49.683357229873835	17.633904999548196
17	14.706987043611457	19.153812069767014	26.266162825256956	39.873038061364575
18	22.023356554891823	24.17992407541073	33.36436073215952	20.432358637537927
19	28.60214491863745	27.185989115370756	24.12418362558966	20.08768234040214
20	31.71185235748354	24.629875792423437	23.684683242918407	19.973588607174623
21	20.76712794066255	29.207015893648965	28.2100021447009	21.815854020987587
22	21.89968243185131	24.83618077760105	25.856056820421035	27.408079970126604
23	18.130778853031376	32.40991439703966	22.551584411399357	26.907722338529606
24	20.29061241553199	25.369743169345366	37.88717566842271	16.452468746699935
25	16.21883787694207	25.872169294197437	35.168086850588374	22.74090597827211
26	15.68908586750974	37.28774809671403	25.74501139304311	21.27815464273311
27	17.61583290058277	35.48260669342644	28.96924803738094	17.932312368609853
28	13.26350535576451	30.46270016754795	40.21379510812232	16.05999936856522
29	13.415376307913728	26.130295478818084	38.92599475473658	21.52833345853161
30	17.956589947340518	31.23892947853285	33.32288199899188	17.48159857513475
31	30.78865115732198	27.87828114742587	22.95722682552695	18.375840869725206
32	30.0680534280921	28.66136914657234	24.784141842025903	16.486435583309657
33	27.373568793186603	29.92086380278332	23.55719873756591	19.148368666464172
34	18.188478928041473	30.652130602486764	27.290611326851327	23.868779142620433
35	17.75627270579603	27.871531327330345	30.52584364586089	23.846352321012734
36	31.58828710250908	23.268916098647534	27.622550060258476	17.52024673858491
37	19.444380938072577	31.507506997494943	31.037196952129626	18.010915112302854
38	19.555535233516558	33.371763760651376	22.798388317150096	24.274312688681967
39	18.85801753429072	32.20491582865473	28.014366229996853	20.922700407057697
40	22.590885783245856	27.62004609473917	25.032578768767493	24.75648935324748
41	16.2610786865721	23.609782013471335	28.822711620468507	31.30642767948806
42	23.436246316176813	25.172583101716523	24.420740237528346	26.97043034457832
43	23.83731627153002	26.63239499947199	25.260221894892236	24.27006683410575
44	19.402466732640715	32.613933152830086	26.42500133363381	21.558598780895398
45	17.046561783171825	39.274808038382524	20.47187774551654	23.206752432929107
46	24.091849809970793	32.221246038563244	24.774561452212907	18.912342699253056
47	21.5651308648588	28.436229985966904	27.032376274164626	22.96626287500966
48	24.091740941904735	23.81162340794062	29.83115651635253	22.265479133802117
49	20.656191381350684	22.587619741264152	31.155318803801237	25.600870073583927
50	18.37507879326281	31.95310399186538	28.10222275930468	21.56959445556713
51	17.532439961983272	34.40905869404045	23.68174380513487	24.376757538841403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1134.0
1	1319.5
2	1505.0
3	3708.5
4	5912.0
5	4648.5
6	3385.0
7	3698.5
8	4012.0
9	4468.5
10	4925.0
11	5258.5
12	5592.0
13	5500.0
14	5408.0
15	5117.0
16	4826.0
17	4662.5
18	4499.0
19	4558.5
20	4618.0
21	4219.5
22	3821.0
23	4118.0
24	4415.0
25	4813.0
26	5485.5
27	5760.0
28	6232.5
29	6705.0
30	8651.5
31	10598.0
32	12482.5
33	14367.0
34	16678.0
35	18989.0
36	20317.0
37	21645.0
38	29219.0
39	36793.0
40	50684.0
41	64575.0
42	81056.5
43	97538.0
44	100569.0
45	103600.0
46	100050.5
47	96501.0
48	89833.5
49	83166.0
50	75245.5
51	67325.0
52	62414.5
53	57504.0
54	49136.5
55	40769.0
56	37627.0
57	34485.0
58	31531.5
59	28578.0
60	25713.5
61	22849.0
62	19838.0
63	16827.0
64	14933.5
65	13040.0
66	9769.5
67	6499.0
68	5386.5
69	4274.0
70	3526.5
71	2779.0
72	2325.5
73	1872.0
74	1555.0
75	938.0
76	638.0
77	402.5
78	167.0
79	135.5
80	104.0
81	78.0
82	52.0
83	36.0
84	20.0
85	14.5
86	9.0
87	8.5
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	918543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.17876067663382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.6927110787601	16.081001445304246
2	8.248950173849408	3.329071827831683
3	2.6037125669772068	1.5761907827933088
4	1.1078707008537914	0.8942183093273295
5	0.6444110129668601	0.6501707804022723
6	0.4375794375818883	0.529788644878857
7	0.30553983930103246	0.43157907061028833
8	0.2063790581534	0.3331578898517242
9	0.19916727031817605	0.3617053814132018
>10	3.113376334513359	17.23398178641566
>50	2.8634558697485732	41.825846411853966
>100	0.5719256902342956	15.412640699599342
>500	0.0038274185770937564	0.4789522257876969
>1k	0.0010935481648839303	0.8616947439304107
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4020	0.4376496255482868	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3790	0.41260997035522556	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07642538237186501	0.0
2	0.0	0.0	0.0	0.25649316362979196	0.0
3	0.0	0.0	0.0	0.3674297229416587	0.0
4	0.0	0.0	0.0	0.6848890035632518	0.0
5	0.0	0.0	0.0	1.0780115900943124	0.0
6	0.0	0.0	0.0	1.5658493940947784	0.0
7	0.0	0.0	0.0	1.7864160959258304	0.0
8	0.0	0.0	0.0	2.3490462613073095	0.0
9	0.0	0.0	0.0	2.5188804443558985	0.0
10	0.0	0.0	0.0	2.855173900405316	0.0
11	0.0	0.0	0.0	3.3019684435023726	0.0
12	0.0	0.0	0.0	3.627701697144282	0.0
13	0.0	0.0	0.0	3.7545329941004395	0.0
14	0.0	0.0	0.0	3.805592117081073	0.0
15	0.0	0.0	0.0	3.889638264076913	0.0
16	0.0	0.0	0.0	4.0903909778856296	0.0
17	0.0	0.0	0.0	4.319013916604884	0.0
18	0.0	0.0	0.0	4.672290790959161	0.0
19	0.0	0.0	0.0	4.804674359284214	0.0
20	0.0	0.0	0.0	4.954041345914127	0.0
21	0.0	0.0	0.0	5.155120663921014	0.0
22	0.0	0.0	0.0	5.327132208290739	0.0
23	0.0	0.0	0.0	5.546936833659394	0.0
24	0.0	0.0	0.0	5.688247583401104	0.0
25	0.0	0.0	0.0	5.8150788803572615	0.0
26	0.0	0.0	0.0	5.93951507986017	0.0
27	0.0	0.0	0.0	6.046423520727935	0.0
28	0.0	0.0	0.0	6.164001032069266	0.0
29	0.0	0.0	0.0	6.298126489451229	0.0
30	0.0	0.0	0.0	6.4818957849550864	0.0
31	0.0	0.0	0.0	6.675463206404055	0.0
32	0.0	0.0	0.0	6.818515845202675	0.0
33	0.0	0.0	0.0	6.956233948764511	0.0
34	0.0	0.0	0.0	7.1199715201139195	0.0
35	0.0	0.0	0.0	7.360134473835194	0.0
36	0.0	0.0	0.0	7.522347892259807	0.0
37	0.0	0.0	0.0	7.687391880401897	0.0
38	0.0	0.0	0.0	7.851564924015533	0.0
39	0.0	0.0	0.0	8.05503933947567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	35	1.2115743E-7	45.000004	1
TCGATAG	35	1.2115743E-7	45.000004	1
CGCCTAA	85	0.0	45.000004	30
TAGGACG	35	1.2115743E-7	45.000004	1
CACCGGG	60	0.0	45.000004	3
AGTCGGT	30	2.165023E-6	45.000004	12
ATTTACG	25	3.8901915E-5	45.0	1
ACAACGA	20	7.032547E-4	45.0	14
CTTAACG	20	7.032547E-4	45.0	1
CGGAATA	20	7.032547E-4	45.0	10
CGGGTAT	45	3.8380676E-10	45.0	6
AGGTACG	25	3.8901915E-5	45.0	1
GTCGATG	25	3.8901915E-5	45.0	1
CCCTCCG	20	7.032547E-4	45.0	44
CGAAACG	20	7.032547E-4	45.0	35
CGACGCA	20	7.032547E-4	45.0	14
ACGTTAG	55	1.8189894E-12	45.0	1
TTTGTCG	20	7.032547E-4	45.0	1
CGTGATT	20	7.032547E-4	45.0	13
GCTATTG	50	2.1827873E-11	45.0	1
>>END_MODULE