FastQCFastQC Report
Thu 26 May 2016
SRR1547908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547908_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3320903
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152020.4577670591402399No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50130.15095291852848458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG207.0352154E-445.031
CGACGGT6100.041.31147428
CACGACG6000.041.2526
TCACGAC6150.040.9756125
CGTTTTT80300.040.4887921
ATACGTA451.9301297E-840.012
TACGGGA11700.039.4230774
TACGTTG1600.039.3751
CGGTCTA6400.039.02343831
CGTTAGG4750.038.8421062
GGGCGAT39300.037.6717537
TAGACGC301.1403863E-437.50000412
ACGGGAC9350.037.2994655
TTAGGGA39400.037.2906074
ACGGGAT14250.037.1052675
TACGCGG2850.037.105262
CTCACGA6800.037.05882324
ACGTTAG2250.037.01
AGGGCGA19100.036.8717276
GACCGAT13800.036.8478289
Produced by FastQC (version 0.11.5)