##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547908_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3320903
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.452834665752057	31.0	31.0	33.0	30.0	34.0
2	31.885090591324108	31.0	31.0	34.0	30.0	34.0
3	31.959324316307946	33.0	31.0	34.0	30.0	34.0
4	35.731805174676886	37.0	35.0	37.0	35.0	37.0
5	35.6427363280409	37.0	35.0	37.0	33.0	37.0
6	35.67960973265404	37.0	35.0	37.0	33.0	37.0
7	35.9852157681209	37.0	35.0	37.0	35.0	37.0
8	35.99769791529593	37.0	35.0	37.0	35.0	37.0
9	37.73183077012487	39.0	38.0	39.0	35.0	39.0
10	37.26176103306842	39.0	37.0	39.0	34.0	39.0
11	37.006683423153284	39.0	37.0	39.0	33.0	39.0
12	36.370486581511116	38.0	35.0	39.0	32.0	39.0
13	36.12337397388602	38.0	35.0	39.0	32.0	39.0
14	37.01415127150658	39.0	35.0	41.0	31.0	41.0
15	37.257204441081235	39.0	35.0	41.0	32.0	41.0
16	37.393205402265586	39.0	35.0	41.0	32.0	41.0
17	37.34744013902243	39.0	35.0	41.0	32.0	41.0
18	37.25416400298353	39.0	35.0	41.0	32.0	41.0
19	37.20049908112342	39.0	35.0	41.0	32.0	41.0
20	37.03737868886866	39.0	35.0	41.0	32.0	41.0
21	36.92064266857538	39.0	35.0	41.0	32.0	41.0
22	36.87659892505141	39.0	35.0	41.0	31.0	41.0
23	36.79124111725034	38.0	35.0	41.0	31.0	41.0
24	36.83194600986539	38.0	35.0	41.0	32.0	41.0
25	36.78396779430173	38.0	35.0	41.0	31.0	41.0
26	36.676448243143504	38.0	35.0	41.0	31.0	41.0
27	36.60179053709187	38.0	35.0	41.0	31.0	41.0
28	36.52595152583499	38.0	35.0	41.0	31.0	41.0
29	36.520354554167945	38.0	35.0	41.0	31.0	41.0
30	36.42685769503054	38.0	35.0	40.0	31.0	41.0
31	36.246971079853886	38.0	35.0	40.0	30.0	41.0
32	36.12106195212567	38.0	35.0	40.0	30.0	41.0
33	36.02007917725992	38.0	35.0	40.0	30.0	41.0
34	35.88139039291422	38.0	35.0	40.0	29.0	41.0
35	35.78953676153745	38.0	35.0	40.0	29.0	41.0
36	35.66835737147397	38.0	35.0	40.0	29.0	41.0
37	35.56317122180322	38.0	34.0	40.0	28.0	41.0
38	35.41984333779096	38.0	34.0	40.0	27.0	41.0
39	35.34071937662738	38.0	34.0	40.0	27.0	41.0
40	35.18474402895839	38.0	34.0	40.0	26.0	41.0
41	35.097069381430295	38.0	34.0	40.0	26.0	41.0
42	35.03879366545786	37.0	34.0	40.0	26.0	41.0
43	34.97415311437883	37.0	34.0	40.0	26.0	41.0
44	34.81822112840995	37.0	34.0	40.0	25.0	41.0
45	34.684632161794546	37.0	34.0	40.0	24.0	41.0
46	34.66615736743891	37.0	34.0	40.0	24.0	41.0
47	34.567458911025106	37.0	34.0	40.0	24.0	41.0
48	34.462076429212175	36.0	34.0	40.0	24.0	41.0
49	34.33530247646499	36.0	34.0	40.0	24.0	41.0
50	34.26225638026766	36.0	33.0	40.0	24.0	41.0
51	33.92694487011514	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	10.0
11	6.0
12	14.0
13	17.0
14	51.0
15	117.0
16	317.0
17	749.0
18	1639.0
19	3031.0
20	5301.0
21	8227.0
22	12341.0
23	17803.0
24	25822.0
25	36253.0
26	46803.0
27	53878.0
28	60309.0
29	68351.0
30	80179.0
31	97211.0
32	120429.0
33	157850.0
34	275294.0
35	385672.0
36	231339.0
37	303523.0
38	478509.0
39	849736.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.044478866139723	25.23647935516334	30.8177324059149	13.901309372782041
2	31.27068149837559	26.590177430656663	28.625617791305558	13.513523279662188
3	29.36406152182102	25.57223140814411	30.925413961202718	14.138293108832144
4	26.733752837707094	27.03282811933983	30.617726564130297	15.61569247882278
5	23.722463438408166	31.620736889936264	29.202569301181036	15.454230370474537
6	22.301404166276463	40.37787312667669	26.6310096982658	10.689713008781046
7	88.4177586638333	3.664816467087416	6.147123237264082	1.770301631815202
8	88.91976067955011	2.9357978838888097	5.870029928606767	2.2744115079543126
9	83.91687441638615	5.448969753106309	7.6312978728978225	3.0028579576097227
10	44.26865223103475	31.314284096825475	13.623794492040266	10.793269180099509
11	36.83284335615946	21.293124189414748	25.10214842167928	16.771884032746513
12	34.966694299713055	20.34678519667693	27.592886633545156	17.09363387006486
13	25.03033060586232	29.185013835092445	28.25936198678492	17.525293572260317
14	19.14045667699418	32.44084515567001	30.70682883541013	17.711869331925683
15	17.48072738047453	25.513512439237157	40.23556243587964	16.770197744408673
16	22.11877311682997	22.683800159173575	36.90550431614534	18.291922407851118
17	22.50607741328187	22.66302267786804	27.86148827592977	26.969411632920327
18	23.995822822888833	25.45587751283311	31.323679131850586	19.224620532427476
19	27.265566022253584	26.739925857515264	26.321154216187587	19.67335390404357
20	27.222324771304674	27.10332099431992	26.280863969829895	19.393490264545516
21	24.025453317968033	26.058394358401916	30.849922445792604	19.066229877837443
22	23.524806355379845	23.148764056041387	29.923818913108875	23.402610675469894
23	20.55070563638866	27.81174276996347	28.70381941297292	22.933732180674955
24	21.338714199119938	26.952337963499684	32.074499014274124	19.634448823106247
25	21.98474330626339	26.6363094616133	29.770758134158086	21.608189097965223
26	19.809792697949923	30.09097224459733	26.8421872002886	23.25704785716415
27	19.649384519812834	29.09148505692578	30.478728225425435	20.78040219783595
28	18.007993608967197	27.18007722598341	33.7398593093505	21.072069855698885
29	19.629570631843208	26.354307849401202	32.40970904600345	21.60641247275214
30	22.19218688410953	26.396284384096734	31.121113745267476	20.290414986526255
31	25.862363339127942	25.964444008150796	27.240512595519956	20.93268005720131
32	26.9775720639838	26.269511635841212	26.90966884609397	19.843247454081016
33	25.33461531396732	25.901057634023033	27.622547240916102	21.14177981109355
34	20.32667018578983	26.358764468579782	30.530732153272773	22.783833192357623
35	20.61773559781782	27.0701673611063	30.026682501717154	22.285414539358722
36	26.488789344343992	25.059720202607544	28.122471508502358	20.329018944546107
37	21.040271275613893	28.78783270694748	29.056434349332093	21.115461668106537
38	21.216398070042995	29.14665077540657	26.667897255656065	22.96905389889437
39	20.24030813305899	27.58376260914577	28.973806220777902	23.20212303701734
40	22.562387398849047	25.234371494741037	29.01289197546571	23.190349130944206
41	19.31622212392232	24.69310305058594	29.512244109508767	26.47843071598297
42	22.06959974440687	26.359095703789	27.517455342718534	24.0538492090856
43	21.72357939994032	25.997958988865378	28.320128591530676	23.958333019663627
44	21.597800357312455	27.29203472669933	28.268757021810032	22.841407894178182
45	19.953639115626082	29.168271400881025	26.352380662729384	24.52570882076351
46	22.597769341651954	27.191007987887634	28.146561341900078	22.064661328560334
47	21.315015825514926	26.921322303000117	29.2156380357993	22.548023835685655
48	21.765284924010125	26.48797631246682	29.229489690002993	22.517249073520063
49	21.659801566019844	24.863026712915133	30.021924759621104	23.45524696144392
50	20.109801460626823	27.359094800420248	29.78999988858452	22.741103850368408
51	19.554530800809296	29.254362442986135	27.360570302715857	23.830536453488705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2085.0
1	3659.0
2	5233.0
3	15363.5
4	25494.0
5	18907.5
6	12321.0
7	11583.5
8	10846.0
9	11600.5
10	12355.0
11	12547.5
12	12740.0
13	12655.0
14	12570.0
15	12182.5
16	11795.0
17	11818.0
18	11841.0
19	11438.5
20	11036.0
21	12641.0
22	14246.0
23	15717.5
24	17189.0
25	21214.0
26	28599.5
27	31960.0
28	36720.0
29	41480.0
30	51805.5
31	62131.0
32	71155.5
33	80180.0
34	88873.5
35	97567.0
36	101871.0
37	106175.0
38	117891.5
39	129608.0
40	159400.5
41	189193.0
42	215157.0
43	241121.0
44	251792.0
45	262463.0
46	273613.0
47	284763.0
48	285382.0
49	286001.0
50	274602.5
51	263204.0
52	238815.0
53	214426.0
54	196102.5
55	177779.0
56	171862.5
57	165946.0
58	148820.5
59	131695.0
60	119744.5
61	107794.0
62	96335.0
63	84876.0
64	74916.0
65	64956.0
66	53263.0
67	41570.0
68	34396.0
69	27222.0
70	23104.0
71	18986.0
72	15181.0
73	11376.0
74	9502.5
75	5293.0
76	2957.0
77	2183.0
78	1409.0
79	1097.5
80	786.0
81	543.5
82	301.0
83	244.5
84	188.0
85	130.5
86	73.0
87	60.5
88	48.0
89	33.5
90	19.0
91	16.5
92	14.0
93	10.5
94	7.0
95	8.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3320903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.834084746377997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53793836704122	14.603561022642886
2	8.596369863550935	3.238095170426563
3	3.006424818648544	1.6986977945412232
4	1.5259456815604875	1.14959161139519
5	0.9523056341511941	0.8967902509028415
6	0.6558045279253178	0.741088683360231
7	0.4730644928562324	0.6236815724269626
8	0.35563639475516456	0.5358468798172087
9	0.298879828272207	0.5066215213195484
>10	3.3011398155224483	15.189518071785852
>50	2.0826993080541114	29.24063877598645
>100	1.2053433042257362	29.578858833156758
>500	0.006011050906704961	0.7718654356559882
>1k	0.002111990859112554	0.6066055760675481
>5k	1.624608353163503E-4	0.15338783116401605
>10k+	1.624608353163503E-4	0.46515096935076244
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15202	0.4577670591402399	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5013	0.15095291852848458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0112291747154314E-5	0.0	0.0	0.020928042764272247	0.0
2	3.0112291747154314E-5	0.0	0.0	0.12538758283515056	0.0
3	3.0112291747154314E-5	0.0	0.0	0.23063004249145488	0.0
4	3.0112291747154314E-5	0.0	0.0	0.4560807708023992	0.0
5	3.0112291747154314E-5	0.0	0.0	0.8914442848827563	0.0
6	3.0112291747154314E-5	0.0	0.0	1.4158799579511958	0.0
7	3.0112291747154314E-5	0.0	0.0	1.6967674153686512	0.0
8	3.0112291747154314E-5	0.0	0.0	2.3364428289534502	0.0
9	3.0112291747154314E-5	0.0	0.0	2.620130729503391	0.0
10	3.0112291747154314E-5	0.0	0.0	3.107769182056808	0.0
11	3.0112291747154314E-5	0.0	0.0	3.6719530802314915	0.0
12	3.0112291747154314E-5	0.0	0.0	4.125715204569359	0.0
13	3.0112291747154314E-5	0.0	0.0	4.3090689490177825	0.0
14	6.022458349430863E-5	0.0	0.0	4.393353253618067	0.0
15	6.022458349430863E-5	0.0	0.0	4.50136604411511	0.0
16	6.022458349430863E-5	0.0	0.0	4.720101731366438	0.0
17	6.022458349430863E-5	0.0	0.0	4.974098912253686	0.0
18	6.022458349430863E-5	0.0	0.0	5.308495912105834	0.0
19	6.022458349430863E-5	0.0	0.0	5.4838698992412604	0.0
20	6.022458349430863E-5	0.0	0.0	5.684598436027791	0.0
21	6.022458349430863E-5	0.0	0.0	5.92883923438896	0.0
22	6.022458349430863E-5	0.0	0.0	6.198494806984726	0.0
23	6.022458349430863E-5	0.0	0.0	6.461465450812625	0.0
24	6.022458349430863E-5	0.0	0.0	6.663247917810306	0.0
25	6.022458349430863E-5	0.0	0.0	6.830310912423519	0.0
26	6.022458349430863E-5	0.0	0.0	7.00679905435359	0.0
27	6.022458349430863E-5	0.0	0.0	7.212646680737137	0.0
28	6.022458349430863E-5	0.0	0.0	7.409310058137802	0.0
29	6.022458349430863E-5	0.0	0.0	7.634339214364286	0.0
30	6.022458349430863E-5	0.0	0.0	7.894148067558733	0.0
31	6.022458349430863E-5	0.0	0.0	8.108848707715943	0.0
32	6.022458349430863E-5	0.0	0.0	8.311353869715557	0.0
33	6.022458349430863E-5	0.0	0.0	8.52009227610683	0.0
34	6.022458349430863E-5	0.0	0.0	8.755991969654037	0.0
35	6.022458349430863E-5	0.0	0.0	9.019625083900372	0.0
36	6.022458349430863E-5	0.0	0.0	9.243088400956005	0.0
37	6.022458349430863E-5	0.0	0.0	9.492568738081179	0.0
38	6.022458349430863E-5	0.0	0.0	9.817480366032974	0.0
39	6.022458349430863E-5	0.0	0.0	10.43601695081127	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATCG	20	7.0352154E-4	45.0	31
CGACGGT	610	0.0	41.311474	28
CACGACG	600	0.0	41.25	26
TCACGAC	615	0.0	40.97561	25
CGTTTTT	8030	0.0	40.488792	1
ATACGTA	45	1.9301297E-8	40.0	12
TACGGGA	1170	0.0	39.423077	4
TACGTTG	160	0.0	39.375	1
CGGTCTA	640	0.0	39.023438	31
CGTTAGG	475	0.0	38.842106	2
GGGCGAT	3930	0.0	37.671753	7
TAGACGC	30	1.1403863E-4	37.500004	12
ACGGGAC	935	0.0	37.299465	5
TTAGGGA	3940	0.0	37.290607	4
ACGGGAT	1425	0.0	37.105267	5
TACGCGG	285	0.0	37.10526	2
CTCACGA	680	0.0	37.058823	24
ACGTTAG	225	0.0	37.0	1
AGGGCGA	1910	0.0	36.871727	6
GACCGAT	1380	0.0	36.847828	9
>>END_MODULE