FastQCFastQC Report
Thu 26 May 2016
SRR1547907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547907_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2338956
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400561.712558936551179No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40450.1729404058904913No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA207.034785E-445.00000422
CGTTTTT179300.043.3561061
ATTAGCG1350.040.01
CGACGGT3500.039.85714328
ACGGGAT11200.039.575895
CTATGCG403.4602635E-739.3750041
CGGTTTT17900.038.715081
CGGTCTA3600.038.12531
TCGTCCC4750.037.89473738
TAAGGGA32000.037.828134
ATAGGGA35050.037.746084
TCGATAG1850.037.70271
ACGTAGG3000.037.52
TACGGGA8900.037.415734
CGAGGGA16400.037.3170744
ACGGGAC7800.037.2115365
AGGGATT40700.037.039316
TAGGGAT40900.037.0232285
GGGCGAT30350.036.9192777
AAGGGAT35250.036.893625