##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547907_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2338956
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.446381205973946	31.0	31.0	33.0	30.0	34.0
2	31.816309926309003	31.0	31.0	34.0	30.0	34.0
3	31.626238373017706	31.0	31.0	34.0	30.0	34.0
4	35.563638221497115	37.0	35.0	37.0	33.0	37.0
5	35.58767031102766	37.0	35.0	37.0	33.0	37.0
6	35.66586716466663	37.0	35.0	37.0	33.0	37.0
7	35.99904487301172	37.0	35.0	37.0	35.0	37.0
8	36.03768604454295	37.0	35.0	37.0	35.0	37.0
9	37.78917859079008	39.0	38.0	39.0	35.0	39.0
10	37.353331571863684	39.0	37.0	39.0	34.0	39.0
11	37.074085190144665	39.0	37.0	39.0	33.0	39.0
12	36.735187408399305	39.0	35.0	39.0	33.0	39.0
13	36.645342622948014	39.0	35.0	39.0	33.0	39.0
14	37.745737842011565	40.0	36.0	41.0	33.0	41.0
15	37.888315556171214	40.0	36.0	41.0	33.0	41.0
16	37.940212214338366	40.0	36.0	41.0	33.0	41.0
17	37.85912817513455	40.0	36.0	41.0	33.0	41.0
18	37.66837212841969	39.0	36.0	41.0	33.0	41.0
19	37.52528136484825	39.0	36.0	41.0	33.0	41.0
20	37.276681989742436	39.0	35.0	41.0	32.0	41.0
21	37.18186319024385	39.0	35.0	41.0	32.0	41.0
22	37.120978761464514	39.0	35.0	41.0	32.0	41.0
23	37.01446756587127	39.0	35.0	41.0	32.0	41.0
24	37.02446903661291	39.0	35.0	41.0	32.0	41.0
25	36.98153535166972	39.0	35.0	41.0	32.0	41.0
26	36.88402774571219	39.0	35.0	41.0	32.0	41.0
27	36.807084870343864	39.0	35.0	41.0	31.0	41.0
28	36.725575855210614	38.0	35.0	41.0	31.0	41.0
29	36.695482086879785	38.0	35.0	41.0	31.0	41.0
30	36.56547964134426	38.0	35.0	41.0	31.0	41.0
31	36.372442234911645	38.0	35.0	41.0	30.0	41.0
32	36.147813383406955	38.0	35.0	41.0	30.0	41.0
33	35.916455461325484	38.0	35.0	41.0	29.0	41.0
34	35.63569943171227	38.0	35.0	41.0	27.0	41.0
35	35.42066759699627	38.0	35.0	41.0	25.0	41.0
36	35.29465624834328	38.0	35.0	41.0	25.0	41.0
37	35.18728740514999	38.0	34.0	40.0	24.0	41.0
38	35.057733450308596	38.0	34.0	40.0	24.0	41.0
39	34.95235053588011	38.0	34.0	40.0	23.0	41.0
40	34.816601081850195	38.0	34.0	40.0	23.0	41.0
41	34.70856185409217	38.0	34.0	40.0	23.0	41.0
42	34.646150248230406	38.0	34.0	40.0	23.0	41.0
43	34.59692230208692	38.0	34.0	40.0	23.0	41.0
44	34.415746170513685	38.0	34.0	40.0	22.0	41.0
45	34.28478047470752	38.0	33.0	40.0	22.0	41.0
46	34.25983173689458	37.0	33.0	40.0	22.0	41.0
47	34.18333393189098	37.0	33.0	40.0	22.0	41.0
48	34.08374078007453	37.0	33.0	40.0	21.0	41.0
49	33.94608064452687	37.0	33.0	40.0	20.0	41.0
50	33.87673987881773	37.0	33.0	40.0	20.0	41.0
51	33.57245668580341	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	7.0
12	3.0
13	14.0
14	25.0
15	90.0
16	198.0
17	505.0
18	992.0
19	1936.0
20	3410.0
21	5609.0
22	8678.0
23	13068.0
24	20639.0
25	32495.0
26	45712.0
27	51635.0
28	51096.0
29	51155.0
30	55347.0
31	64423.0
32	76938.0
33	98697.0
34	157148.0
35	216143.0
36	168058.0
37	219264.0
38	353985.0
39	641587.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.764751453212458	22.590933732827807	28.27034796721272	18.37396684674701
2	34.891806429877256	24.701191471750644	27.534036553060425	12.872965545311668
3	27.411631514231132	24.356379512910888	35.164962487537174	13.0670264853208
4	24.19446966937386	26.631582637723838	35.01318536988297	14.16076232301933
5	21.85650349985207	30.55893313084983	33.42606701451417	14.158496354783928
6	20.58076338332145	38.801841505355384	30.634394148500444	9.98300096282273
7	84.42014300397271	2.822498584838706	11.104612485228452	1.6527459259601294
8	85.01083389341227	2.199400074221148	10.878143924041323	1.911622108325253
9	80.19223106377376	4.846179235522173	12.316349687638418	2.645240013065658
10	38.59345793593381	35.38852376872417	16.881335091382653	9.136683203959374
11	27.14364870480676	23.211167717562876	32.30860264152041	17.336580936109957
12	26.914101847148896	21.388559682182994	33.48737641922293	18.209962051445174
13	23.337078594039394	24.54086353056663	33.82684411335656	18.29521376203742
14	17.467066503174923	28.71824010370439	34.51967458985975	19.29501880326094
15	17.150814294924743	26.13730228358293	39.30672488067326	17.405158540819066
16	22.024740952801164	24.530944575272045	36.30538582170849	17.1389286502183
17	21.24405076452913	24.545096188213886	32.21727984622199	21.99357320103499
18	21.88647413632407	25.324161720015255	34.44780491809166	18.341559225569014
19	23.53148156698972	27.09020605774542	30.746666461446903	18.63164591381796
20	24.12499422819412	26.887295015382932	30.980745255575563	18.006965500847386
21	23.21279237403354	25.52339590825993	33.65544285570144	17.6083688620051
22	22.27109017869511	22.50247546341188	33.53419217804866	21.69224217984434
23	19.673050711514026	26.73928026008185	33.18288159332625	20.40478743507787
24	19.774591740930568	25.898349520042274	35.31139534048524	19.015663398541914
25	20.790814363331332	26.925901983620044	32.49325767564674	19.790025977401886
26	19.371206640911588	28.14896902720701	31.472417608539878	21.007406723341525
27	18.799968875002353	27.457292911880344	34.07439045454468	19.668347758572626
28	17.940867634961926	26.847961227145788	35.41152548401937	19.799645653872926
29	19.214341783257147	25.88975594239481	34.480768342799095	20.415133931548947
30	20.949090106868194	25.369951380017408	34.027147154542455	19.653811358571943
31	23.15776782461919	24.893413984700867	31.508160050894503	20.44065813978544
32	23.51750097051847	25.886079088276993	30.907763976748605	19.688655964455936
33	21.923712972796412	25.621944149441035	31.068947000285597	21.38539587747696
34	19.572364764450466	26.146066877700992	32.52976969211905	21.751798665729495
35	19.16162595619584	27.360882376581692	32.01757536268318	21.459916304539288
36	22.490247785764247	26.818247115379684	30.248110695541087	20.443394403314983
37	19.897296058583404	29.150954528430635	30.341229163780763	20.6105202492052
38	19.871045030346874	29.39721824608928	29.570457930803318	21.16127879276053
39	19.423366664443453	28.70520009782142	29.73536056257578	22.136072675159344
40	21.9380783563265	26.647529923606943	30.294840946131522	21.119550773935035
41	19.15174975501891	27.023039338918732	30.873817207335236	22.951393698727124
42	20.91005559745459	27.35070689658121	29.44189629903256	22.29734120693164
43	20.575248102144716	27.16160543421937	29.836003755521695	22.427142708114218
44	20.672000670384566	28.436362206044063	29.78187704257797	21.1097600809934
45	19.887377103288816	28.560007114285174	28.866126596652524	22.686489185773482
46	21.19582411982098	27.94640001778571	29.51782761197731	21.339948250415997
47	20.36887397625265	27.40359374011311	30.64422759555973	21.58330468807451
48	20.727153482151866	26.66706855537257	31.005371627341425	21.60040633513414
49	20.90402726686607	26.121012964758638	30.689333189679495	22.285626578695794
50	19.136614797371134	27.201537780103603	31.686957770902918	21.974889651622348
51	18.84101282794546	27.793596801307935	30.84850676968699	22.516883601059618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1353.0
1	2360.0
2	3367.0
3	33037.5
4	62708.0
5	44404.0
6	26100.0
7	23096.5
8	20093.0
9	20120.0
10	20147.0
11	20125.0
12	20103.0
13	19477.5
14	18852.0
15	17508.0
16	16164.0
17	15413.5
18	14663.0
19	14075.5
20	13488.0
21	13942.5
22	14397.0
23	14769.5
24	15142.0
25	16803.5
26	22156.5
27	25848.0
28	29197.5
29	32547.0
30	39098.5
31	45650.0
32	52017.0
33	58384.0
34	65626.5
35	72869.0
36	78218.5
37	83568.0
38	90194.5
39	96821.0
40	110044.5
41	123268.0
42	134881.0
43	146494.0
44	152548.5
45	158603.0
46	168964.0
47	179325.0
48	185834.0
49	192343.0
50	183569.0
51	174795.0
52	159298.5
53	143802.0
54	131949.0
55	120096.0
56	113477.5
57	106859.0
58	99418.5
59	91978.0
60	83311.0
61	74644.0
62	64316.5
63	53989.0
64	45200.5
65	36412.0
66	29316.5
67	22221.0
68	18170.0
69	14119.0
70	11360.5
71	8602.0
72	6731.5
73	4861.0
74	3766.5
75	2113.0
76	1554.0
77	1133.5
78	713.0
79	572.0
80	431.0
81	300.0
82	169.0
83	170.5
84	172.0
85	112.0
86	52.0
87	39.0
88	26.0
89	19.5
90	13.0
91	9.5
92	6.0
93	5.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2338956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.8711066893469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.20238154900623	18.454953481089596
2	7.037949019264078	3.500831618646025
3	2.988387415004003	2.2297350668299845
4	1.7492770352051565	1.740258230872475
5	1.2151165005366815	1.5110646062416826
6	0.9148820116837806	1.3652476872450972
7	0.7491658026191246	1.3042807823465312
8	0.6563144441132488	1.305861324904001
9	0.5714363992746508	1.2791030087282467
>10	9.402112510716695	55.62428687115797
>50	0.4705353694436525	7.061934012034852
>100	0.03702786213663829	1.602975215976561
>500	0.0036676032554594858	0.6323099957317285
>1k	0.0015718299666254937	0.6472522667032004
>5k	0.0	0.0
>10k+	1.746477740694993E-4	1.7399058314920741
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40056	1.712558936551179	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4045	0.1729404058904913	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044378774119735474	0.0
2	0.0	0.0	0.0	0.1374117341241135	0.0
3	0.0	0.0	0.0	0.25891893648277264	0.0
4	0.0	0.0	0.0	0.3949197847244668	0.0
5	0.0	0.0	0.0	0.751403617682419	0.0
6	0.0	0.0	0.0	1.339144473004195	0.0
7	0.0	0.0	0.0	1.726967074198916	0.0
8	0.0	0.0	0.0	2.454855927174346	0.0
9	0.0	0.0	0.0	2.9340013236674825	0.0
10	0.0	0.0	0.0	3.5037426954589996	0.0
11	0.0	0.0	0.0	3.973866973128182	0.0
12	0.0	0.0	0.0	4.309657813143984	0.0
13	0.0	0.0	0.0	4.4888403202112395	0.0
14	0.0	0.0	0.0	4.61120260492288	0.0
15	0.0	0.0	0.0	4.70782691080978	0.0
16	0.0	0.0	0.0	4.860373602581665	0.0
17	0.0	0.0	0.0	5.050928704943573	0.0
18	0.0	0.0	0.0	5.296251832013941	0.0
19	0.0	0.0	0.0	5.432209926137986	0.0
20	0.0	0.0	0.0	5.58296094496861	0.0
21	0.0	0.0	0.0	5.761587648506428	0.0
22	0.0	0.0	0.0	5.961463148515834	0.0
23	0.0	0.0	0.0	6.1828867238203715	0.0
24	0.0	0.0	0.0	6.3502690944164835	0.0
25	0.0	0.0	0.0	6.501105621482405	0.0
26	0.0	0.0	0.0	6.647495720312824	0.0
27	0.0	0.0	0.0	6.814621566203041	0.0
28	0.0	0.0	0.0	6.98307278974038	0.0
29	0.0	0.0	0.0	7.176449663867127	0.0
30	0.0	0.0	0.0	7.426090956820051	0.0
31	0.0	0.0	0.0	7.617843174476134	0.0
32	0.0	0.0	0.0	7.8142128368383155	0.0
33	0.0	0.0	0.0	8.000749052141211	0.0
34	0.0	0.0	0.0	8.20832029332745	0.0
35	0.0	0.0	0.0	8.436413510985243	0.0
36	0.0	0.0	0.0	8.626498318053011	0.0
37	0.0	0.0	0.0	8.832402148650937	0.0
38	4.275411764907078E-5	0.0	0.0	9.0848652133687	0.0
39	4.275411764907078E-5	0.0	0.0	9.479015423975483	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGA	20	7.034785E-4	45.000004	22
CGTTTTT	17930	0.0	43.356106	1
ATTAGCG	135	0.0	40.0	1
CGACGGT	350	0.0	39.857143	28
ACGGGAT	1120	0.0	39.57589	5
CTATGCG	40	3.4602635E-7	39.375004	1
CGGTTTT	1790	0.0	38.71508	1
CGGTCTA	360	0.0	38.125	31
TCGTCCC	475	0.0	37.894737	38
TAAGGGA	3200	0.0	37.82813	4
ATAGGGA	3505	0.0	37.74608	4
TCGATAG	185	0.0	37.7027	1
ACGTAGG	300	0.0	37.5	2
TACGGGA	890	0.0	37.41573	4
CGAGGGA	1640	0.0	37.317074	4
ACGGGAC	780	0.0	37.211536	5
AGGGATT	4070	0.0	37.03931	6
TAGGGAT	4090	0.0	37.023228	5
GGGCGAT	3035	0.0	36.919277	7
AAGGGAT	3525	0.0	36.89362	5
>>END_MODULE