FastQCFastQC Report
Thu 26 May 2016
SRR1547906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547906_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2441934
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92120.37724197296077616No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38830.15901330666594593No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT207.0348475E-445.032
ACCGTAG406.8212103E-945.01
GACGATT207.0348475E-445.035
CTAGACG502.1827873E-1144.9999961
CGGTCTA5200.043.70192731
TAGCCGT800.042.187544
ACGTGCG650.041.5384641
CGTTTTT48950.041.1389161
CATACGA2550.040.58823418
AATCGTC451.9297659E-840.025
TACGGGA9400.039.9734044
CGACGGT5800.039.95689428
TCGTTAG403.4603545E-739.3751
TCTCACG6300.038.57142623
CTATGCG1700.038.3823551
CTCACGA6450.038.37209324
TACGCGG2000.038.2499962
TATGGGA20200.038.094064
AAGTACG950.037.8947371
CACGACG6250.037.826
Produced by FastQC (version 0.11.5)