##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547906_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2441934
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.428544342312282	31.0	31.0	33.0	30.0	34.0
2	31.83572365182679	31.0	31.0	34.0	30.0	34.0
3	31.928234751635383	33.0	31.0	34.0	30.0	34.0
4	35.69195768599806	37.0	35.0	37.0	33.0	37.0
5	35.57290655685207	37.0	35.0	37.0	33.0	37.0
6	35.6134772684274	37.0	35.0	37.0	33.0	37.0
7	35.97458326064505	37.0	35.0	37.0	35.0	37.0
8	35.998468836586085	37.0	35.0	37.0	35.0	37.0
9	37.73015036442426	39.0	38.0	39.0	35.0	39.0
10	37.2451548649554	39.0	37.0	39.0	34.0	39.0
11	36.945718844162045	39.0	37.0	39.0	33.0	39.0
12	36.211328807412485	38.0	35.0	39.0	32.0	39.0
13	35.88837413296182	38.0	35.0	39.0	31.0	39.0
14	36.667121633918036	39.0	35.0	41.0	31.0	41.0
15	36.97725818961528	39.0	35.0	41.0	31.0	41.0
16	37.15478755773088	39.0	35.0	41.0	32.0	41.0
17	37.118430719257766	39.0	35.0	41.0	32.0	41.0
18	37.06886426905887	38.0	35.0	41.0	32.0	41.0
19	36.99791435804571	38.0	35.0	41.0	32.0	41.0
20	36.83577156466964	38.0	35.0	41.0	31.0	41.0
21	36.708755437288644	38.0	35.0	41.0	31.0	41.0
22	36.666663800086326	38.0	35.0	41.0	31.0	41.0
23	36.61834717891638	38.0	35.0	40.0	31.0	41.0
24	36.633568311019054	38.0	35.0	41.0	31.0	41.0
25	36.58729146651793	38.0	35.0	41.0	31.0	41.0
26	36.48176936805008	38.0	35.0	40.0	31.0	41.0
27	36.39785514268608	38.0	35.0	40.0	31.0	41.0
28	36.34231678661258	38.0	35.0	40.0	31.0	41.0
29	36.34740783329934	38.0	35.0	40.0	31.0	41.0
30	36.255934435574424	38.0	35.0	40.0	30.0	41.0
31	36.079749493639056	38.0	35.0	40.0	30.0	41.0
32	35.98258593393597	38.0	35.0	40.0	30.0	41.0
33	35.91201809713121	38.0	35.0	40.0	30.0	41.0
34	35.76901791776518	38.0	35.0	40.0	30.0	41.0
35	35.73623242888628	38.0	35.0	40.0	29.0	41.0
36	35.60126399812608	38.0	34.0	40.0	29.0	41.0
37	35.4698140899795	38.0	34.0	40.0	28.0	41.0
38	35.33803493460511	38.0	34.0	40.0	27.0	41.0
39	35.27313965078499	37.0	34.0	40.0	27.0	41.0
40	35.10374072354126	37.0	34.0	40.0	26.0	41.0
41	35.02839429730697	37.0	34.0	40.0	26.0	41.0
42	34.963615724257906	37.0	34.0	40.0	26.0	41.0
43	34.91304433289352	37.0	34.0	40.0	26.0	41.0
44	34.750438791548014	37.0	34.0	40.0	26.0	41.0
45	34.628045229723654	36.0	34.0	40.0	25.0	41.0
46	34.61080192994569	36.0	34.0	40.0	25.0	41.0
47	34.50594242104823	36.0	34.0	40.0	24.0	41.0
48	34.407187090232576	36.0	34.0	40.0	24.0	41.0
49	34.26473156113146	36.0	33.0	40.0	24.0	41.0
50	34.1719845827119	36.0	33.0	40.0	24.0	41.0
51	33.83123253945438	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	5.0
12	8.0
13	18.0
14	34.0
15	77.0
16	252.0
17	564.0
18	1181.0
19	2232.0
20	3848.0
21	5966.0
22	8853.0
23	12961.0
24	18612.0
25	26254.0
26	34181.0
27	40487.0
28	45671.0
29	51908.0
30	62162.0
31	75154.0
32	93505.0
33	122530.0
34	215176.0
35	314771.0
36	165525.0
37	213480.0
38	334089.0
39	592339.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.2563042244385	24.127597224167403	29.980621916890467	12.635476634503634
2	29.522992840920352	26.91637038511278	29.967640403057576	13.592996370909288
3	29.181419317639218	25.64156115603452	30.931630420805806	14.245389105520461
4	26.34944269583044	27.904112068548947	30.101304949273818	15.645140286346807
5	23.532863705571074	31.81973796179586	29.72095068908496	14.926447643548105
6	21.84997628928546	40.59532321512375	26.698633132590803	10.856067362999983
7	88.21986998829617	3.662179239897557	6.137512316057682	1.9804384557485992
8	88.36618843916338	2.883534116810692	5.736477726261234	3.0137997177646896
9	83.25384715557423	5.287653147054752	8.047842406879138	3.410657290491881
10	46.46169798200934	30.277026324216788	12.719958852286753	10.541316841487117
11	39.254336931301175	21.08566406790683	24.04790629066961	15.612092710122386
12	36.583052613215585	20.3980123950934	26.618942199093016	16.399992792598
13	24.941951748081642	32.13448848330872	27.271007324522284	15.65255244408735
14	17.738071544931188	35.86083817171144	29.723776318278873	16.6773139650785
15	16.114849950899572	25.24400741379579	42.877858287734234	15.763284347570409
16	21.07907093312104	22.42955788321879	38.77623228146215	17.715138902198014
17	20.989879333348078	22.870724597798304	26.806785113766384	29.33261095508724
18	23.464557191144397	24.691781186551314	31.9960736039549	19.84758801834939
19	26.652890700567667	27.558074870164383	25.937064638110613	19.851969791157337
20	27.354506714759697	27.55402070653834	25.198797346693237	19.892675232008727
21	23.014831686687685	27.163182952528608	30.699027901655	19.122957459128706
22	22.0874929461648	23.177407743206818	29.69277629944134	25.042323011187033
23	19.30375677639117	28.843695202245435	28.192408148623183	23.660139872740213
24	20.988364140881778	27.076202714733487	33.15355779476432	18.781875349620424
25	20.598468263270014	26.03276747037389	30.84215216299867	22.526612103357422
26	18.674296684513177	30.788792817496297	26.604936906566678	23.931973591423848
27	18.95382921897152	29.84675261493554	30.52203704113215	20.67738112496079
28	16.44118964722224	27.819752704209037	34.6590448390497	21.080012809519015
29	18.033411222416333	26.129371227887404	33.40082082480526	22.436396724891008
30	21.450047380477933	26.767431060790344	31.27693049853108	20.505591060200643
31	26.036412122522556	25.36182386583749	26.946223771813653	21.6555402398263
32	26.857728341552228	26.46988002132736	26.668779745889935	20.003611891230474
33	25.77031156452222	25.936245615155855	26.435644861818542	21.857797958503383
34	18.773726071220597	26.572872157887968	30.543741149433195	24.109660621458236
35	19.221936383211013	27.090863225623625	30.22260224887323	23.464598142292132
36	27.65385960472314	24.329322577923893	27.934374966727194	20.082442850625775
37	20.543511823005865	29.52712890684187	28.965852475947347	20.96350679420492
38	20.7139914510384	29.17417096448962	27.23750928567275	22.874328298799227
39	19.679974970658503	27.64325325745905	28.636564296987547	24.0402074748949
40	22.772564696670752	24.868198731005833	28.045311625948944	24.31392494637447
41	18.729867391993395	23.889384397776517	28.976786432393343	28.403961777836745
42	21.561434502324797	25.59618728434102	28.47333302210461	24.369045191229574
43	21.304425099122255	25.486806768733306	29.117003162247627	24.09176496989681
44	20.901875316859506	27.57253062531583	28.573499529471313	22.952094528353346
45	18.86754515068794	29.634134255880788	25.603558490933825	25.89476210249745
46	22.38905719810609	27.21605088425813	28.10849924690839	22.28639267072738
47	20.35333469291144	26.608622509863082	29.255540895044664	23.782501902180815
48	21.466919253345914	25.409859562133946	30.10965898341233	23.01356220110781
49	21.892155971455413	22.888865956246153	31.15387229957894	24.06510577271949
50	19.500322285532697	27.23750928567275	29.751377391854163	23.510791036940393
51	18.633755048252738	29.065322813802503	26.593224878313666	25.707697259631097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1616.0
1	2477.5
2	3339.0
3	9209.5
4	15080.0
5	11215.0
6	7350.0
7	7268.5
8	7187.0
9	7685.5
10	8184.0
11	8449.5
12	8715.0
13	8758.5
14	8802.0
15	8257.5
16	7713.0
17	7647.5
18	7582.0
19	7627.0
20	7672.0
21	8585.0
22	9498.0
23	9961.0
24	10424.0
25	13484.5
26	18313.5
27	20082.0
28	25485.0
29	30888.0
30	34855.5
31	38823.0
32	44664.5
33	50506.0
34	56244.0
35	61982.0
36	66299.5
37	70617.0
38	82587.5
39	94558.0
40	120244.0
41	145930.0
42	171374.0
43	196818.0
44	207385.0
45	217952.0
46	226535.5
47	235119.0
48	237334.0
49	239549.0
50	225370.0
51	211191.0
52	184259.5
53	157328.0
54	143534.0
55	129740.0
56	122407.5
57	115075.0
58	104202.0
59	93329.0
60	82057.0
61	70785.0
62	59194.5
63	47604.0
64	42527.0
65	37450.0
66	30671.5
67	23893.0
68	18727.0
69	13561.0
70	11068.5
71	8576.0
72	6551.0
73	4526.0
74	3891.5
75	2425.0
76	1593.0
77	1153.5
78	714.0
79	532.0
80	350.0
81	269.5
82	189.0
83	151.5
84	114.0
85	87.5
86	61.0
87	48.5
88	36.0
89	28.0
90	20.0
91	13.0
92	6.0
93	3.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2441934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.809078092565514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.96202844428949	15.047377535226131
2	9.6800687435527	3.8350647536487585
3	3.4747874211960017	2.0649700614460778
4	1.778955069777126	1.4095783960152166
5	1.0562849920739124	1.0462015947998538
6	0.689150424298042	0.8190860743466741
7	0.4938807865428808	0.6848326148331924
8	0.3887749172190592	0.6161018156498429
9	0.3366372835708098	0.6001626815210963
>10	3.159971372120349	14.676924453371127
>50	1.8299504319119246	27.133026394475113
>100	1.1392090933557748	29.581007914067197
>500	0.006096522095297637	0.8314980522968413
>1k	0.0039942730969191416	1.270546680802155
>5k	2.1022489983784954E-4	0.38362097750080143
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9212	0.37724197296077616	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3883	0.15901330666594593	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2285344321345294E-4	0.0	0.0	0.11433560448398687	0.0
2	1.2285344321345294E-4	0.0	0.0	0.4319527063385005	0.0
3	1.2285344321345294E-4	0.0	0.0	0.6376093702778208	0.0
4	1.2285344321345294E-4	0.0	0.0	1.1055171843301252	0.0
5	1.2285344321345294E-4	0.0	0.0	1.8955876776358411	0.0
6	1.2285344321345294E-4	0.0	0.0	2.6972473457513595	0.0
7	1.2285344321345294E-4	0.0	0.0	3.1320256812837695	0.0
8	1.2285344321345294E-4	0.0	0.0	4.176402802041332	0.0
9	1.2285344321345294E-4	0.0	0.0	4.511792702014059	0.0
10	1.2285344321345294E-4	0.0	0.0	5.1476821240868915	0.0
11	1.2285344321345294E-4	0.0	0.0	5.98349504941575	0.0
12	1.2285344321345294E-4	0.0	0.0	6.635027809924429	0.0
13	1.2285344321345294E-4	0.0	0.0	6.8847888599773786	0.0
14	1.2285344321345294E-4	0.0	0.0	6.988067654572155	0.0
15	1.2285344321345294E-4	0.0	0.0	7.14409152745324	0.0
16	1.2285344321345294E-4	0.0	0.0	7.4964352025894225	0.0
17	1.2285344321345294E-4	0.0	0.0	7.903407708807855	0.0
18	1.2285344321345294E-4	0.0	0.0	8.459729050826107	0.0
19	1.2285344321345294E-4	0.0	0.0	8.741391044966818	0.0
20	1.2285344321345294E-4	0.0	0.0	9.042095322805613	0.0
21	1.2285344321345294E-4	0.0	0.0	9.416347861981528	0.0
22	1.2285344321345294E-4	0.0	0.0	9.804400937945088	0.0
23	1.2285344321345294E-4	0.0	0.0	10.208056401196757	0.0
24	1.2285344321345294E-4	0.0	0.0	10.501798983920123	0.0
25	1.2285344321345294E-4	0.0	0.0	10.73714522996936	0.0
26	1.2285344321345294E-4	0.0	0.0	10.976095177019527	0.0
27	1.2285344321345294E-4	0.0	0.0	11.258658096410468	0.0
28	1.2285344321345294E-4	0.0	0.0	11.51493037895373	0.0
29	1.2285344321345294E-4	0.0	0.0	11.826200052908883	0.0
30	1.2285344321345294E-4	0.0	0.0	12.185464472012757	0.0
31	1.2285344321345294E-4	0.0	0.0	12.48227839081646	0.0
32	1.2285344321345294E-4	0.0	0.0	12.754275914091044	0.0
33	1.2285344321345294E-4	0.0	0.0	13.039336853493992	0.0
34	1.2285344321345294E-4	0.0	0.0	13.350729380892359	0.0
35	1.2285344321345294E-4	0.0	0.0	13.713720354440374	0.0
36	1.2285344321345294E-4	0.0	0.0	14.000050779423194	0.0
37	1.2285344321345294E-4	0.0	0.0	14.301041715296154	0.0
38	1.2285344321345294E-4	0.0	0.0	14.706867589377927	0.0
39	1.2285344321345294E-4	0.0	0.0	15.460450610049248	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGAT	20	7.0348475E-4	45.0	32
ACCGTAG	40	6.8212103E-9	45.0	1
GACGATT	20	7.0348475E-4	45.0	35
CTAGACG	50	2.1827873E-11	44.999996	1
CGGTCTA	520	0.0	43.701927	31
TAGCCGT	80	0.0	42.1875	44
ACGTGCG	65	0.0	41.538464	1
CGTTTTT	4895	0.0	41.138916	1
CATACGA	255	0.0	40.588234	18
AATCGTC	45	1.9297659E-8	40.0	25
TACGGGA	940	0.0	39.973404	4
CGACGGT	580	0.0	39.956894	28
TCGTTAG	40	3.4603545E-7	39.375	1
TCTCACG	630	0.0	38.571426	23
CTATGCG	170	0.0	38.382355	1
CTCACGA	645	0.0	38.372093	24
TACGCGG	200	0.0	38.249996	2
TATGGGA	2020	0.0	38.09406	4
AAGTACG	95	0.0	37.894737	1
CACGACG	625	0.0	37.8	26
>>END_MODULE