FastQCFastQC Report
Thu 26 May 2016
SRR1547905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547905_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3498114
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190240.5438359070058895No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC207.0352666E-445.021
CGTTTTT97950.040.9800871
TACGGGA15000.040.6499984
CGTAAGG5750.040.3043482
TATAGCG1900.040.2631571
CGAGGGA25750.039.407774
ACGGGAT16800.039.3755
CGGTCTA4650.038.7096831
CGTCGGT356.2519503E-638.5714316
ACCGTTC3350.038.28358545
TAAGGGA45200.037.6327444
TATGGGA40000.037.6312524
AACGGGA14300.037.6048974
TAGGGAT52550.037.5071335
GGGCGAT41050.037.4908647
AGGGCGA22150.037.178336
TAGGGAC41650.037.0048035
AAGGGAT56150.036.8655365
GACCGAT10400.036.7788479
GTAGGGA47000.036.7180864
Produced by FastQC (version 0.11.5)