##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547905_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3498114
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43271945968599	31.0	31.0	33.0	30.0	34.0
2	31.851296727322207	31.0	31.0	34.0	30.0	34.0
3	31.920455708418878	33.0	31.0	34.0	30.0	34.0
4	35.71173066400924	37.0	35.0	37.0	33.0	37.0
5	35.6367070941656	37.0	35.0	37.0	33.0	37.0
6	35.6739548796866	37.0	35.0	37.0	33.0	37.0
7	35.97563286959773	37.0	35.0	37.0	35.0	37.0
8	35.99468685125756	37.0	35.0	37.0	35.0	37.0
9	37.72970892315116	39.0	38.0	39.0	35.0	39.0
10	37.25855217983176	39.0	37.0	39.0	34.0	39.0
11	37.018092892341414	39.0	37.0	39.0	33.0	39.0
12	36.524946013766275	39.0	35.0	39.0	32.0	39.0
13	36.35780995130519	39.0	35.0	39.0	32.0	39.0
14	37.366756200626966	40.0	35.0	41.0	32.0	41.0
15	37.539047612513485	40.0	35.0	41.0	32.0	41.0
16	37.62742552129519	40.0	35.0	41.0	33.0	41.0
17	37.53679011032802	40.0	35.0	41.0	32.0	41.0
18	37.43819126535041	39.0	36.0	41.0	32.0	41.0
19	37.37758031899475	39.0	35.0	41.0	32.0	41.0
20	37.23020404709509	39.0	35.0	41.0	32.0	41.0
21	37.11663170497016	39.0	35.0	41.0	32.0	41.0
22	37.04411434275727	39.0	35.0	41.0	32.0	41.0
23	36.94953509233833	39.0	35.0	41.0	31.0	41.0
24	36.99337928952573	39.0	35.0	41.0	32.0	41.0
25	36.95957278693604	39.0	35.0	41.0	31.0	41.0
26	36.85619708219915	39.0	35.0	41.0	31.0	41.0
27	36.77901149019157	39.0	35.0	41.0	31.0	41.0
28	36.70716591854925	39.0	35.0	41.0	31.0	41.0
29	36.70382726234765	39.0	35.0	41.0	31.0	41.0
30	36.604902527476234	38.0	35.0	41.0	31.0	41.0
31	36.456041741349765	38.0	35.0	41.0	30.0	41.0
32	36.33512801469592	38.0	35.0	41.0	30.0	41.0
33	36.22763494843221	38.0	35.0	41.0	30.0	41.0
34	36.068865394323915	38.0	35.0	41.0	30.0	41.0
35	35.99158575163646	38.0	35.0	41.0	29.0	41.0
36	35.899460966680905	38.0	35.0	41.0	29.0	41.0
37	35.809879266370395	38.0	35.0	40.0	29.0	41.0
38	35.6896822116146	38.0	35.0	40.0	28.0	41.0
39	35.585034964555184	38.0	35.0	40.0	27.0	41.0
40	35.42744804771943	38.0	34.0	40.0	27.0	41.0
41	35.354308350156685	38.0	34.0	40.0	27.0	41.0
42	35.29749602214222	38.0	34.0	40.0	27.0	41.0
43	35.243977754870194	38.0	34.0	40.0	26.0	41.0
44	35.06758041618998	38.0	34.0	40.0	26.0	41.0
45	34.93338267420673	37.0	34.0	40.0	26.0	41.0
46	34.89744044933927	37.0	34.0	40.0	26.0	41.0
47	34.81525244746169	37.0	34.0	40.0	25.0	41.0
48	34.70433153407808	37.0	34.0	40.0	25.0	41.0
49	34.567815685823845	37.0	34.0	40.0	24.0	41.0
50	34.49169009357614	37.0	34.0	40.0	24.0	41.0
51	34.157884505765104	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	7.0
12	14.0
13	18.0
14	50.0
15	114.0
16	289.0
17	704.0
18	1421.0
19	2666.0
20	4800.0
21	7616.0
22	11464.0
23	16872.0
24	24954.0
25	36065.0
26	47523.0
27	55507.0
28	61330.0
29	69611.0
30	81811.0
31	98952.0
32	122099.0
33	160319.0
34	267066.0
35	356834.0
36	255641.0
37	341245.0
38	537699.0
39	935292.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.596446542336814	23.982265872410107	28.948484812101604	13.472802773151477
2	31.299065725130742	25.738755226387706	29.172605581178885	13.789573467302665
3	29.544491688950103	25.333251003254897	31.262617513322894	13.859639794472105
4	25.97179508729561	28.094396008820755	30.484369577435157	15.44943932644848
5	23.457383035544296	31.921429661812052	28.87756087994845	15.7436264226952
6	21.824560320218268	40.90369839290543	26.37352584850008	10.898215438376223
7	88.6068321386896	3.2476357259940642	6.276925223134524	1.8686069121818214
8	88.85256455335646	2.7161207439208668	6.095084379754347	2.3362303229683192
9	83.60302151387862	5.633178335525944	7.819127678514766	2.9446724720806694
10	41.53521011607969	34.88193923925864	13.32938263304169	10.253468011619976
11	33.71119408915776	22.010660601684222	26.428241046461036	17.849904262696985
12	31.40758134240336	20.51971433749729	29.367967996468952	18.704736323630392
13	24.512980423165168	27.153088778696176	29.48568857390011	18.848242224238547
14	18.96782094580108	30.608407844913	30.94335976471893	19.480411444566986
15	18.301747741783146	26.450167147211324	37.38445916856912	17.86362594243641
16	23.286205080794968	24.17314015495207	34.7854872654236	17.75516749882937
17	22.84902664693032	24.113393674419985	28.078387382458093	24.959192296191603
18	24.23180033583811	25.535931647739325	30.41433183709851	19.817936179324057
19	26.18902642967039	27.392760784811472	26.252117569638955	20.166095215879185
20	27.015900568134715	26.723600202852165	26.932026800727478	19.328472428285643
21	25.40185940195202	26.079710381079636	29.71409736789596	18.804332849072384
22	24.229170347221388	22.724902618954097	29.6581815229578	23.38774551086671
23	21.63651613412256	26.69421293874356	29.246216675614345	22.423054251519535
24	21.79182839667318	25.687985011351834	32.266987296583245	20.253199295391745
25	22.223975547966702	26.17093096451402	29.489719317323566	22.11537417019571
26	20.427836256908723	29.15708293097366	26.985055375553795	23.430025436563817
27	19.980881126229736	27.946659256959606	30.180834586865952	21.89162502994471
28	18.559858255048294	27.717735899973526	31.995469558739366	21.726936286238814
29	20.14153912651217	26.289451973263304	31.431394174117827	22.137614726106698
30	21.723963255628604	25.923569100378092	30.517387369308146	21.835080274685158
31	24.763715533570373	25.892009236977408	27.35285356623598	21.991421663216233
32	25.394198130764178	26.655363432981318	26.67929061202694	21.27114782422757
33	23.619356030135098	26.06973357643576	27.315547749444413	22.99536264398473
34	19.81367674123828	27.288847647618113	29.736509444803687	23.160966166339918
35	20.16392261658711	27.43132442224582	29.205594786219084	23.199158174947986
36	24.22205222585656	26.925623350182416	27.505735947999412	21.346588475961617
37	20.864843169776627	29.084872591344933	28.24807882190232	21.80220541697612
38	21.453731925260296	28.72753718146407	27.20294421508276	22.61578667819288
39	20.969356630458584	27.60315987414933	27.807126926109326	23.620356569282762
40	23.001194357873985	25.296945725611003	28.288729298130367	23.41313061838465
41	19.69501279832504	25.12385245306471	29.719900494952423	25.461234253657828
42	21.64020383555253	25.978227124673463	27.986737996531847	24.394831043242156
43	21.473742708213624	26.071906175727833	27.91032539248292	24.54402572357562
44	21.127213121127557	27.453393457160058	28.15560041782515	23.263793003887237
45	20.460825461948925	28.3420151544518	26.579093763096346	24.618065620502936
46	21.74005764248964	27.80612638696166	27.2750973810459	23.1787185895028
47	20.796349118410664	26.67208673016374	29.098079708094133	23.433484443331466
48	21.392527516255903	25.91101948078307	29.635540751387744	23.060912251573278
49	21.683398539898928	24.63358827070816	29.584656189020713	24.0983570003722
50	20.215178807780422	27.042743604124965	29.28426575005846	23.457811838036154
51	19.346310611946897	27.763617766602234	28.088907336924983	24.80116428452589
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1638.0
1	3003.5
2	4369.0
3	18082.0
4	31795.0
5	23167.5
6	14540.0
7	13302.5
8	12065.0
9	12424.0
10	12783.0
11	12814.0
12	12845.0
13	12764.0
14	12683.0
15	12318.0
16	11953.0
17	11951.5
18	11950.0
19	12398.0
20	12846.0
21	14277.5
22	15709.0
23	17887.0
24	20065.0
25	22941.5
26	31545.0
27	37272.0
28	42293.0
29	47314.0
30	54977.5
31	62641.0
32	73745.5
33	84850.0
34	95994.5
35	107139.0
36	114290.5
37	121442.0
38	132660.5
39	143879.0
40	163610.0
41	183341.0
42	207328.5
43	231316.0
44	240165.5
45	249015.0
46	258777.0
47	268539.0
48	277111.0
49	285683.0
50	278330.5
51	270978.0
52	249988.0
53	228998.0
54	211610.0
55	194222.0
56	187226.5
57	180231.0
58	171457.0
59	162683.0
60	147587.5
61	132492.0
62	117799.5
63	103107.0
64	88146.0
65	73185.0
66	61518.0
67	49851.0
68	40522.0
69	31193.0
70	25505.5
71	19818.0
72	16488.0
73	13158.0
74	9952.5
75	5559.0
76	4371.0
77	3225.5
78	2080.0
79	1471.5
80	863.0
81	598.0
82	333.0
83	249.0
84	165.0
85	124.5
86	84.0
87	57.0
88	30.0
89	21.5
90	13.0
91	12.0
92	11.0
93	10.0
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3498114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.41157990259094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15124713547392	14.976275983547927
2	8.248623697519129	3.202376359815954
3	2.8578722923578197	1.6642744906351365
4	1.4287445330810702	1.1093675465717272
5	0.8606636834211354	0.8353420929993802
6	0.633865256606341	0.7382595645654179
7	0.49754854969856893	0.6760742399524409
8	0.3939500060437288	0.6117753615955221
9	0.3216959738507745	0.5620164390671504
>10	4.3811655294784435	22.020923657135768
>50	2.5923470984941996	36.42314773336284
>100	0.6255053288844092	15.286667616831023
>500	0.004213013834009652	0.5685549654994362
>1k	0.002407436476576944	0.7692987381223492
>5k	0.0	0.0
>10k+	1.50464779786059E-4	0.5556452102980207
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19024	0.5438359070058895	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8586832790469378E-5	0.0	0.0	0.054057700806777596	0.0
2	2.8586832790469378E-5	0.0	0.0	0.17983976508484287	0.0
3	2.8586832790469378E-5	0.0	0.0	0.28238073430425653	0.0
4	2.8586832790469378E-5	0.0	0.0	0.463964296189318	0.0
5	2.8586832790469378E-5	0.0	0.0	0.8587198701929096	0.0
6	2.8586832790469378E-5	0.0	0.0	1.3756269807101769	0.0
7	2.8586832790469378E-5	0.0	0.0	1.6811058759091329	0.0
8	2.8586832790469378E-5	0.0	0.0	2.3594142443613904	0.0
9	2.8586832790469378E-5	0.0	0.0	2.6755846150239817	0.0
10	2.8586832790469378E-5	0.0	0.0	3.1540424354380674	0.0
11	2.8586832790469378E-5	0.0	0.0	3.6477942113950546	0.0
12	2.8586832790469378E-5	0.0	0.0	4.033259064741744	0.0
13	2.8586832790469378E-5	0.0	0.0	4.208667870744064	0.0
14	2.8586832790469378E-5	0.0	0.0	4.303033005785403	0.0
15	2.8586832790469378E-5	0.0	0.0	4.41066243124152	0.0
16	2.8586832790469378E-5	0.0	0.0	4.6158015433459285	0.0
17	2.8586832790469378E-5	0.0	0.0	4.864564162288593	0.0
18	2.8586832790469378E-5	0.0	0.0	5.170529033644987	0.0
19	2.8586832790469378E-5	0.0	0.0	5.344937300499641	0.0
20	2.8586832790469378E-5	0.0	0.0	5.538184290163214	0.0
21	2.8586832790469378E-5	0.0	0.0	5.782230081695451	0.0
22	2.8586832790469378E-5	0.0	0.0	6.049088165794482	0.0
23	2.8586832790469378E-5	0.0	0.0	6.325151210052045	0.0
24	2.8586832790469378E-5	0.0	0.0	6.532634442445272	0.0
25	2.8586832790469378E-5	0.0	0.0	6.715761693301019	0.0
26	2.8586832790469378E-5	0.0	0.0	6.892914296103558	0.0
27	2.8586832790469378E-5	0.0	0.0	7.089477358370825	0.0
28	2.8586832790469378E-5	0.0	0.0	7.2938160391571	0.0
29	2.8586832790469378E-5	0.0	0.0	7.516021490437419	0.0
30	2.8586832790469378E-5	0.0	0.0	7.801260908020722	0.0
31	2.8586832790469378E-5	0.0	0.0	8.044048878910179	0.0
32	2.8586832790469378E-5	0.0	0.0	8.267226282505373	0.0
33	2.8586832790469378E-5	0.0	0.0	8.48442903804736	0.0
34	2.8586832790469378E-5	0.0	0.0	8.72504440964474	0.0
35	2.8586832790469378E-5	0.0	0.0	9.000650064577655	0.0
36	2.8586832790469378E-5	0.0	0.0	9.233918620147886	0.0
37	2.8586832790469378E-5	0.0	0.0	9.481080376454283	0.0
38	2.8586832790469378E-5	0.0	0.0	9.774209759887757	0.0
39	2.8586832790469378E-5	0.0	0.0	10.238202642909865	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	20	7.0352666E-4	45.0	21
CGTTTTT	9795	0.0	40.980087	1
TACGGGA	1500	0.0	40.649998	4
CGTAAGG	575	0.0	40.304348	2
TATAGCG	190	0.0	40.263157	1
CGAGGGA	2575	0.0	39.40777	4
ACGGGAT	1680	0.0	39.375	5
CGGTCTA	465	0.0	38.70968	31
CGTCGGT	35	6.2519503E-6	38.57143	16
ACCGTTC	335	0.0	38.283585	45
TAAGGGA	4520	0.0	37.632744	4
TATGGGA	4000	0.0	37.631252	4
AACGGGA	1430	0.0	37.604897	4
TAGGGAT	5255	0.0	37.507133	5
GGGCGAT	4105	0.0	37.490864	7
AGGGCGA	2215	0.0	37.17833	6
TAGGGAC	4165	0.0	37.004803	5
AAGGGAT	5615	0.0	36.865536	5
GACCGAT	1040	0.0	36.778847	9
GTAGGGA	4700	0.0	36.718086	4
>>END_MODULE