FastQCFastQC Report
Thu 26 May 2016
SRR1547904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547904_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2024527
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74980.3703581132778175No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39870.1969348889888848No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA207.034561E-445.036
TACGACG207.034561E-445.01
CGAATCG207.034561E-445.024
CATACGA207.034561E-445.018
TATCGAT207.034561E-445.012
CGGGTAC650.041.538466
TACGGGA6300.041.4285744
GTTCACG603.6379788E-1241.2500041
TCACGAC3750.040.825
CGGTCTA3700.040.13513631
CGTTTTT45700.040.1258241
CACGACG3950.039.87341726
CGACGGT3900.039.80769328
TCGTTAG403.4599543E-739.3751
CGAAATC403.4599543E-739.37527
AGTACGG1550.039.193552
ACGTTAT356.250648E-638.57142619
CGATATC356.250648E-638.57142638
GGGCGAT29100.038.0412337
ACGGGAT6350.037.9133875
Produced by FastQC (version 0.11.5)