##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547904_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2024527
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51280175566935	31.0	31.0	33.0	30.0	34.0
2	31.9254853108899	31.0	31.0	34.0	30.0	34.0
3	32.000340326407105	33.0	31.0	34.0	30.0	34.0
4	35.76603473305123	37.0	35.0	37.0	35.0	37.0
5	35.673788988736625	37.0	35.0	37.0	33.0	37.0
6	35.71370843658791	37.0	35.0	37.0	33.0	37.0
7	36.02229557817703	37.0	35.0	37.0	35.0	37.0
8	36.04436344884509	37.0	35.0	37.0	35.0	37.0
9	37.77212702028672	39.0	38.0	39.0	35.0	39.0
10	37.32646045224391	39.0	37.0	39.0	34.0	39.0
11	37.06134519322291	39.0	37.0	39.0	33.0	39.0
12	36.37177869201053	38.0	35.0	39.0	33.0	39.0
13	36.10329079335568	38.0	35.0	39.0	32.0	39.0
14	37.03437840048564	39.0	35.0	41.0	32.0	41.0
15	37.27959419657036	39.0	35.0	41.0	32.0	41.0
16	37.4242260043951	39.0	35.0	41.0	33.0	41.0
17	37.36646288244118	39.0	35.0	41.0	33.0	41.0
18	37.28284186874267	39.0	35.0	41.0	32.0	41.0
19	37.219637969757876	39.0	35.0	41.0	32.0	41.0
20	37.08489736121079	39.0	35.0	41.0	32.0	41.0
21	36.94572016080793	39.0	35.0	41.0	32.0	41.0
22	36.875633666530504	38.0	35.0	41.0	32.0	41.0
23	36.81392443765877	38.0	35.0	41.0	32.0	41.0
24	36.828972397009274	38.0	35.0	41.0	32.0	41.0
25	36.79891401794098	38.0	35.0	41.0	32.0	41.0
26	36.70446924145739	38.0	35.0	41.0	32.0	41.0
27	36.60374843111502	38.0	35.0	41.0	31.0	41.0
28	36.56112810547847	38.0	35.0	41.0	31.0	41.0
29	36.56867900502191	38.0	35.0	41.0	31.0	41.0
30	36.488082401469576	38.0	35.0	40.0	31.0	41.0
31	36.312079315316616	38.0	35.0	40.0	31.0	41.0
32	36.186547277462836	38.0	35.0	40.0	30.0	41.0
33	36.09011191256032	38.0	35.0	40.0	30.0	41.0
34	35.934232045312314	38.0	35.0	40.0	30.0	41.0
35	35.87761783369646	38.0	35.0	40.0	30.0	41.0
36	35.75381014923486	38.0	35.0	40.0	29.0	41.0
37	35.64304649925637	38.0	35.0	40.0	29.0	41.0
38	35.51657745241234	38.0	34.0	40.0	29.0	41.0
39	35.45378105601951	38.0	35.0	40.0	28.0	41.0
40	35.28367564374296	38.0	34.0	40.0	27.0	41.0
41	35.22399256715272	38.0	34.0	40.0	27.0	41.0
42	35.17128988647719	37.0	34.0	40.0	27.0	41.0
43	35.09466260514184	37.0	34.0	40.0	27.0	41.0
44	34.9284050052185	37.0	34.0	40.0	26.0	41.0
45	34.80508089049936	37.0	34.0	40.0	26.0	41.0
46	34.79099463726589	37.0	34.0	40.0	26.0	41.0
47	34.70856995238888	37.0	34.0	40.0	26.0	41.0
48	34.61980798477867	36.0	34.0	40.0	25.0	41.0
49	34.496176637802314	36.0	34.0	40.0	24.0	41.0
50	34.410161978575736	36.0	34.0	40.0	24.0	41.0
51	34.10104977607115	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	8.0
13	16.0
14	33.0
15	77.0
16	182.0
17	420.0
18	887.0
19	1727.0
20	2866.0
21	4594.0
22	6866.0
23	10127.0
24	14771.0
25	20794.0
26	27032.0
27	31322.0
28	34969.0
29	39608.0
30	47065.0
31	57563.0
32	71662.0
33	94792.0
34	169106.0
35	261233.0
36	135967.0
37	177566.0
38	286281.0
39	526907.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.08924652523775	23.979428281272614	29.22840742553693	12.702917767952712
2	30.787833405037325	25.872512443647334	29.998068684685357	13.341585466629983
3	29.974161865956837	25.17906651775946	30.749503464265977	14.097268152017731
4	26.70776927153849	28.423626852099282	29.374071079318774	15.494532797043458
5	23.664885674530396	32.36390524799126	28.867928162973378	15.103280914504968
6	22.917204858221204	40.442434208089104	26.471368373946113	10.168992559743586
7	88.16143227529196	4.232593588527098	5.873421297913044	1.7325528382679016
8	88.83576262504774	3.2558716184076575	5.707999942702665	2.2003658138419495
9	83.87746866305068	6.028074705844872	7.116328900528371	2.9781277305760803
10	45.08944558407964	30.857627485333612	13.337880897612134	10.715046032974616
11	39.154330863456	22.485844841782797	23.080601049035156	15.27922324572604
12	35.79166886882714	22.493994893622066	26.05265328642196	15.66168295112883
13	24.664625366814075	33.124082810454	26.566995648860203	15.644296173871725
14	17.975606153931263	36.744977962753765	29.231914417540494	16.047501465774474
15	16.567869927148415	26.20602244376094	42.00018078296807	15.225926846122576
16	20.629114850036576	23.57567965258058	38.797654958417446	16.9975505389654
17	21.27687109137097	22.743090114382273	27.243054797490966	28.736983996755782
18	23.680099104630365	25.549029477008705	31.56174256999289	19.20912884836804
19	27.159627903208992	27.0317955749664	26.205824866746653	19.602751655077952
20	28.61142380417747	26.83846646648822	25.510995901758783	19.039113827575527
21	23.412777404302336	27.40768584464421	30.270971935666946	18.90856481538651
22	23.297392427959714	24.19829421884717	28.64466613683097	23.85964721636214
23	20.31309041568722	28.93391888574467	27.88379705481824	22.869193643749874
24	20.51501412428681	27.012679998834294	33.83817553433469	18.634130342544207
25	20.409903152687022	26.647804647702895	31.4876511896359	21.454641009974182
26	18.858034493983038	32.13451833440601	27.10218238630554	21.905264785305405
27	19.066922792336186	31.535711798360804	30.2686504057491	19.128715003553918
28	17.12992713853656	28.34612726824587	35.26186610502107	19.262079488196502
29	18.327095662344835	26.898529878830956	34.085788927487755	20.688585531336457
30	20.387725132833495	28.318219514978065	31.763765067099627	19.530290285088814
31	26.256157611135833	27.262269162130217	26.85738446560604	19.624188761127908
32	26.97681977074151	28.045217475489338	26.872005164663154	18.105957589105998
33	25.33791843724485	27.885575247946804	26.974300663809377	19.802205650998975
34	19.820827284595367	28.421749870463568	29.784043383960796	21.97337946098027
35	20.1970139197946	28.96513605400175	29.17674103630132	21.661108989902335
36	27.728600310097125	25.894690463500858	28.09036382325353	18.286345403148488
37	21.399319445974292	30.139336249899358	28.72829060812723	19.733053695999114
38	21.196012698274707	31.41459708860391	25.191662052420146	22.197728160701242
39	20.103411809276935	30.05531662457453	27.617315056800923	22.223956509347616
40	23.433226625280867	26.776871832284776	26.946689276063	22.843212266371353
41	19.576819671953004	25.44406668816963	28.584108781952526	26.395004857924835
42	23.00596633188888	26.589766399756588	26.725649991331306	23.678617277023225
43	22.4689520070614	27.0112969597343	27.14678539728045	23.372965635923848
44	20.879642504150354	29.570462631518375	27.898170782607494	21.65172408172378
45	19.35350825155703	32.256077592445045	25.491781537119536	22.898632618878384
46	22.623605414993232	29.23265533134406	27.35947705315859	20.784262200504116
47	21.926603102848222	28.561288636802573	27.50756102536543	22.004547234983775
48	22.717849650807324	26.886823440734553	28.973483682855306	21.421843225602817
49	21.601786491363168	25.07514100824538	30.429280518363054	22.893791982028393
50	20.093582352816238	30.348125759745365	28.05060144912861	21.507690438309787
51	19.517595961920982	31.367771336218286	25.602819819147882	23.511812882712853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1573.0
1	2398.0
2	3223.0
3	8258.5
4	13294.0
5	10125.0
6	6956.0
7	6720.5
8	6485.0
9	6972.5
10	7460.0
11	7743.5
12	8027.0
13	7940.5
14	7854.0
15	7435.0
16	7016.0
17	7131.5
18	7247.0
19	7559.5
20	7872.0
21	8769.0
22	9666.0
23	10096.0
24	10526.0
25	13037.0
26	19114.0
27	22680.0
28	25666.5
29	28653.0
30	33256.5
31	37860.0
32	42217.0
33	46574.0
34	53012.0
35	59450.0
36	63509.0
37	67568.0
38	79683.0
39	91798.0
40	109683.0
41	127568.0
42	150104.5
43	172641.0
44	182912.5
45	193184.0
46	184542.0
47	175900.0
48	172300.0
49	168700.0
50	158277.0
51	147854.0
52	136627.0
53	125400.0
54	116095.5
55	106791.0
56	95739.5
57	84688.0
58	77261.5
59	69835.0
60	63106.5
61	56378.0
62	50254.5
63	44131.0
64	37515.0
65	30899.0
66	26483.5
67	22068.0
68	17457.5
69	12847.0
70	10041.5
71	7236.0
72	5891.0
73	4546.0
74	3626.5
75	2387.5
76	2068.0
77	1440.0
78	812.0
79	652.5
80	493.0
81	377.5
82	262.0
83	178.5
84	95.0
85	68.0
86	41.0
87	37.0
88	33.0
89	22.0
90	11.0
91	8.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2024527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.163212796831207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52391669672365	15.047705252999622
2	8.19710114557485	3.1416558713959937
3	2.76858729003289	1.591650821565075
4	1.3520706537289047	1.036400706150307
5	0.7764374501409985	0.7439518040240487
6	0.5293772251160717	0.6086741048817178
7	0.41809554043547675	0.5608437667539831
8	0.3241513279951066	0.49694247013965254
9	0.2703719570090265	0.46630758118137056
>10	3.379153650012015	16.34281059510952
>50	2.5130830431218842	35.749712968311066
>100	0.9411238794058833	22.77540285872534
>500	0.0049629069345036615	0.6343019065936524
>1k	0.0013060281406588584	0.42832397527792637
>5k	2.6120562813177167E-4	0.3753153168907201
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7498	0.3703581132778175	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3987	0.1969348889888848	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.939425357132802E-5	0.0	0.0	0.04909788804990005	0.0
2	4.939425357132802E-5	0.0	0.0	0.18285752672105632	0.0
3	4.939425357132802E-5	0.0	0.0	0.2776450993244348	0.0
4	4.939425357132802E-5	0.0	0.0	0.45635350874549957	0.0
5	4.939425357132802E-5	0.0	0.0	0.796284761823379	0.0
6	4.939425357132802E-5	0.0	0.0	1.1515776277619414	0.0
7	4.939425357132802E-5	0.0	0.0	1.3324593843401447	0.0
8	4.939425357132802E-5	0.0	0.0	1.7643627375678368	0.0
9	4.939425357132802E-5	0.0	0.0	1.9165464328210984	0.0
10	4.939425357132802E-5	0.0	0.0	2.2351887626097353	0.0
11	4.939425357132802E-5	0.0	0.0	2.6906531747909512	0.0
12	4.939425357132802E-5	0.0	0.0	3.0362647670295333	0.0
13	4.939425357132802E-5	0.0	0.0	3.1749638310578225	0.0
14	4.939425357132802E-5	0.0	0.0	3.2408063710684027	0.0
15	4.939425357132802E-5	0.0	0.0	3.3461149196824738	0.0
16	4.939425357132802E-5	0.0	0.0	3.583898856374847	0.0
17	4.939425357132802E-5	0.0	0.0	3.8580369636957177	0.0
18	4.939425357132802E-5	0.0	0.0	4.20438946973787	0.0
19	4.939425357132802E-5	0.0	0.0	4.382702725130364	0.0
20	4.939425357132802E-5	0.0	0.0	4.578353363526394	0.0
21	4.939425357132802E-5	0.0	0.0	4.817322762304479	0.0
22	4.939425357132802E-5	0.0	0.0	5.067702233657541	0.0
23	4.939425357132802E-5	0.0	0.0	5.32282355335345	0.0
24	4.939425357132802E-5	0.0	0.0	5.519412682567335	0.0
25	4.939425357132802E-5	0.0	0.0	5.683944941213429	0.0
26	4.939425357132802E-5	0.0	0.0	5.858405444827359	0.0
27	4.939425357132802E-5	0.0	0.0	6.051833341812681	0.0
28	4.939425357132802E-5	0.0	0.0	6.231183876530173	0.0
29	4.939425357132802E-5	0.0	0.0	6.452272555515436	0.0
30	4.939425357132802E-5	0.0	0.0	6.687191625500673	0.0
31	4.939425357132802E-5	0.0	0.0	6.906502111357369	0.0
32	4.939425357132802E-5	0.0	0.0	7.098793940510549	0.0
33	4.939425357132802E-5	0.0	0.0	7.292123048988727	0.0
34	4.939425357132802E-5	0.0	0.0	7.528573340834674	0.0
35	4.939425357132802E-5	0.0	0.0	7.787448623802004	0.0
36	4.939425357132802E-5	0.0	0.0	8.006215772869416	0.0
37	4.939425357132802E-5	0.0	0.0	8.234466618622523	0.0
38	4.939425357132802E-5	0.0	0.0	8.48953854406486	0.0
39	4.939425357132802E-5	0.0	0.0	8.913193056946142	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATA	20	7.034561E-4	45.0	36
TACGACG	20	7.034561E-4	45.0	1
CGAATCG	20	7.034561E-4	45.0	24
CATACGA	20	7.034561E-4	45.0	18
TATCGAT	20	7.034561E-4	45.0	12
CGGGTAC	65	0.0	41.53846	6
TACGGGA	630	0.0	41.428574	4
GTTCACG	60	3.6379788E-12	41.250004	1
TCACGAC	375	0.0	40.8	25
CGGTCTA	370	0.0	40.135136	31
CGTTTTT	4570	0.0	40.125824	1
CACGACG	395	0.0	39.873417	26
CGACGGT	390	0.0	39.807693	28
TCGTTAG	40	3.4599543E-7	39.375	1
CGAAATC	40	3.4599543E-7	39.375	27
AGTACGG	155	0.0	39.19355	2
ACGTTAT	35	6.250648E-6	38.571426	19
CGATATC	35	6.250648E-6	38.571426	38
GGGCGAT	2910	0.0	38.041233	7
ACGGGAT	635	0.0	37.913387	5
>>END_MODULE