FastQCFastQC Report
Thu 26 May 2016
SRR1547903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547903_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2050509
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91680.44710849842648825No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48000.2340882190714598No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC207.0345815E-445.027
CTACGCG502.1827873E-1145.01
CGCGTAG207.0345815E-445.02
TCGCGAT207.0345815E-445.01
TACGTAA207.0345815E-445.024
TTTCGCG650.044.9999961
CTAACGG302.1663382E-644.9999962
CGACGGT4150.042.83132628
CGGTCTA4000.042.7531
CGTTTTT42550.042.091661
CGCATCG1350.041.66666421
GACGGTC4350.040.8620729
ACGGTCT4450.039.438230
TACGACG800.039.3751
TTAACGG1600.039.3752
TAGTACG800.039.3751
GGGCGAT25900.038.918927
AGGGATT35000.038.828576
TTACCGG1450.038.7931062
CTCACGA4600.038.64130424
Produced by FastQC (version 0.11.5)