##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547903_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2050509
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.458404230364266	31.0	31.0	33.0	30.0	34.0
2	31.875247072799972	31.0	31.0	34.0	30.0	34.0
3	31.97134906503702	33.0	31.0	34.0	30.0	34.0
4	35.72304583886245	37.0	35.0	37.0	35.0	37.0
5	35.59650262447031	37.0	35.0	37.0	33.0	37.0
6	35.648770134634866	37.0	35.0	37.0	33.0	37.0
7	36.00345036281236	37.0	35.0	37.0	35.0	37.0
8	36.03246462219868	37.0	35.0	37.0	35.0	37.0
9	37.784274538663325	39.0	38.0	39.0	35.0	39.0
10	37.267184391777846	39.0	37.0	39.0	34.0	39.0
11	36.979325133418094	39.0	37.0	39.0	33.0	39.0
12	36.34185609524269	38.0	35.0	39.0	32.0	39.0
13	36.09885691796525	38.0	35.0	39.0	32.0	39.0
14	37.01197897692719	39.0	35.0	41.0	31.0	41.0
15	37.260012026282254	39.0	35.0	41.0	32.0	41.0
16	37.394813677969715	39.0	35.0	41.0	33.0	41.0
17	37.3451567391316	39.0	35.0	41.0	32.0	41.0
18	37.27043675497157	39.0	35.0	41.0	32.0	41.0
19	37.20918952318668	39.0	35.0	41.0	32.0	41.0
20	37.06346424229301	39.0	35.0	41.0	32.0	41.0
21	36.95929693554137	39.0	35.0	41.0	32.0	41.0
22	36.88309097887402	38.0	35.0	41.0	32.0	41.0
23	36.79660367255155	38.0	35.0	41.0	31.0	41.0
24	36.830715690591944	38.0	35.0	41.0	32.0	41.0
25	36.79798235462512	38.0	35.0	41.0	32.0	41.0
26	36.685606354324705	38.0	35.0	41.0	31.0	41.0
27	36.61635428081515	38.0	35.0	41.0	31.0	41.0
28	36.55780979259296	38.0	35.0	41.0	31.0	41.0
29	36.56869684551494	38.0	35.0	41.0	31.0	41.0
30	36.461871174425475	38.0	35.0	40.0	31.0	41.0
31	36.322839841229666	38.0	35.0	40.0	30.0	41.0
32	36.20685839467176	38.0	35.0	40.0	30.0	41.0
33	36.10850135259099	38.0	35.0	40.0	30.0	41.0
34	35.99850232308173	38.0	35.0	40.0	30.0	41.0
35	35.92295912868463	38.0	35.0	40.0	30.0	41.0
36	35.781797105011485	38.0	35.0	40.0	29.0	41.0
37	35.66522165959769	38.0	35.0	40.0	29.0	41.0
38	35.57771655720604	38.0	34.0	40.0	29.0	41.0
39	35.49762180999937	38.0	34.0	40.0	28.0	41.0
40	35.359767257788185	38.0	34.0	40.0	27.0	41.0
41	35.27267571125023	38.0	34.0	40.0	27.0	41.0
42	35.18525205205147	37.0	34.0	40.0	27.0	41.0
43	35.12071197931831	37.0	34.0	40.0	27.0	41.0
44	34.9426620414736	37.0	34.0	40.0	26.0	41.0
45	34.80646707719888	37.0	34.0	40.0	26.0	41.0
46	34.78940594749889	37.0	34.0	40.0	26.0	41.0
47	34.679793163551096	37.0	34.0	40.0	25.0	41.0
48	34.59337023148886	36.0	34.0	40.0	25.0	41.0
49	34.441051465758015	36.0	34.0	40.0	24.0	41.0
50	34.36819687209371	36.0	34.0	40.0	24.0	41.0
51	34.04347554680326	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	0.0
13	13.0
14	21.0
15	64.0
16	141.0
17	331.0
18	804.0
19	1516.0
20	2673.0
21	4269.0
22	6665.0
23	9748.0
24	14459.0
25	20569.0
26	26693.0
27	31760.0
28	35806.0
29	41816.0
30	49610.0
31	60380.0
32	75187.0
33	98327.0
34	172820.0
35	255912.0
36	141573.0
37	181202.0
38	290117.0
39	527941.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.647844998485745	25.18842882425778	29.697504375742806	12.466221801513672
2	29.475169336003894	26.9148294399098	30.691842854627804	12.91815836945851
3	29.355345428866684	25.770918342713934	30.974845757809405	13.898890470609981
4	26.154189033064473	28.012556882218025	30.85721642772599	14.976037656991508
5	23.700505581784814	32.815023001605944	28.668394042649897	14.816077373959343
6	21.79136985012014	40.90311234917769	26.258260753793326	11.047257046908841
7	88.72270250947447	3.5985699160549895	5.826065625656849	1.8526619488136848
8	89.1936099768399	2.8443181668551567	5.35198821365817	2.610083642646777
9	84.53101156834718	5.282298200105437	7.329155833990487	2.857534397556899
10	44.75820393863182	32.20693008418885	13.47158193404662	9.563284043132706
11	36.65894663227521	22.273786654923242	25.13405208170264	15.933214631098913
12	35.13498355774103	22.054524023059642	26.58315569451292	16.227336724686406
13	24.704402662948567	32.830189967466616	26.91243978934011	15.552967580244708
14	17.173540813524838	36.45114456947031	28.775733244769956	17.599581372234894
15	15.502833686660239	27.470788960204516	40.86560946574729	16.160767887387962
16	19.51359394179689	24.3080620470332	38.88351623913867	17.294827772031237
17	19.700328064885355	24.14029882336532	27.48580962092827	28.673563490821056
18	22.336551558661778	26.87396153833024	30.949973884533062	19.839513018474925
19	25.67616138236896	28.396705403390087	26.271038069084312	19.65609514515664
20	26.746432227315264	27.783199195906967	25.718248493422852	19.752120083354914
21	23.098264869844513	28.225138246162295	29.146763071998222	19.529833811994973
22	21.997562556418917	25.434367759419736	28.865857209112466	23.702212475048878
23	19.43541823030282	29.660586712860077	28.17793045531622	22.72606460152089
24	19.77538260012514	27.992366773323113	33.434722793218654	18.79752783333309
25	20.006544716458205	27.9721766644282	30.535003747849927	21.48627487126367
26	18.258393403784133	32.50373443861987	27.145064957042376	22.09280720055362
27	19.051416014267677	31.74577629261808	29.59099423606529	19.611813457048957
28	16.790513965069163	29.706282683957987	33.446378435793264	20.056824915179597
29	17.664443316269278	28.018311550936865	32.90656124893868	21.41068388385518
30	20.93343652722324	29.217623526646307	30.298330804692885	19.550609141437565
31	25.57525960627337	28.924086653606494	25.070799494174373	20.429854245945762
32	26.382522583417096	29.133888219949288	25.715810074474195	18.767779122159425
33	24.651830350415434	28.72808653851312	26.531168602527472	20.088914508543976
34	20.51778363323448	28.238013098211223	29.55407657318256	21.690126695371735
35	20.766160987345092	28.060252356853837	28.99918995722525	22.17439669857582
36	26.32341530810155	26.817634060616168	28.060740040643566	18.798210590638714
37	20.888569618567878	30.695256641155925	29.055810045213164	19.360363695063032
38	20.650970076210346	30.795426891566922	26.455089931329244	22.098513100893484
39	20.441363583383442	28.56471246895283	28.821721826141705	22.172202121522023
40	23.77882759841581	26.41427079812866	27.849134044278763	21.95776755917677
41	19.539685024547563	25.271627678786096	29.144617263323397	26.04407003334294
42	22.17717649617729	27.56140060833676	27.139651666976345	23.121771228509605
43	21.50446547662068	27.4069023837496	27.950035820374357	23.138596319255367
44	20.603859822122214	29.827715947601302	27.487565282571303	22.080858947705178
45	19.46136300791657	31.39649716241187	25.04422072763397	24.09791910203759
46	22.887341630785333	29.418402942879062	26.85786797326908	20.836387453066532
47	21.302710692808468	27.604609392107033	29.06288146016428	22.02979845492022
48	21.90777997072922	26.334632035265393	29.653661603045876	22.103926390959515
49	21.40551443568402	25.087575816541165	30.725541804498295	22.781367943276525
50	19.444440380412864	28.754714073432496	29.201383656448225	22.59946188970641
51	19.148660161940278	30.421032046189506	26.447433295830454	23.982874496039763
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1415.0
1	2079.5
2	2744.0
3	8369.0
4	13994.0
5	10151.5
6	6309.0
7	6317.0
8	6325.0
9	7091.0
10	7857.0
11	8267.0
12	8677.0
13	8794.0
14	8911.0
15	8485.5
16	8060.0
17	7879.0
18	7698.0
19	7464.5
20	7231.0
21	8006.0
22	8781.0
23	9694.5
24	10608.0
25	11243.5
26	16673.0
27	21467.0
28	25698.0
29	29929.0
30	35208.0
31	40487.0
32	44621.5
33	48756.0
34	54862.0
35	60968.0
36	67110.0
37	73252.0
38	83286.0
39	93320.0
40	112559.0
41	131798.0
42	147593.5
43	163389.0
44	174970.0
45	186551.0
46	189839.0
47	193127.0
48	195732.0
49	198337.0
50	180929.0
51	163521.0
52	147335.0
53	131149.0
54	117933.0
55	104717.0
56	94212.5
57	83708.0
58	76151.0
59	68594.0
60	60014.0
61	51434.0
62	44958.0
63	38482.0
64	32057.5
65	25633.0
66	19714.0
67	13795.0
68	10601.5
69	7408.0
70	5985.0
71	4562.0
72	3561.0
73	2560.0
74	2021.0
75	1127.0
76	772.0
77	582.0
78	392.0
79	308.5
80	225.0
81	147.5
82	70.0
83	59.5
84	49.0
85	43.5
86	38.0
87	28.5
88	19.0
89	14.5
90	10.0
91	12.0
92	14.0
93	8.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2050509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.359760733727086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7945060148467	16.403122741418912
2	9.212768672436288	3.9356506907683135
3	3.352766213347512	2.1484285233968112
4	1.6105388073300857	1.3760289430781125
5	0.9842272023276982	1.0511428774672618
6	0.6212328548306841	0.7961631083468176
7	0.48196271251022577	0.7206225755257561
8	0.3754130290407826	0.6414985981307884
9	0.30396946838133043	0.5843443603485096
>10	3.401533095066659	18.141783411138398
>50	2.1807422679707775	34.22117261209969
>100	0.6738580321953062	18.188549543215878
>500	0.004398248735807466	0.6233533846853948
>1k	0.001851894204550512	0.7148266742600741
>5k	2.31486775568814E-4	0.453311956119275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9168	0.44710849842648825	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4800	0.2340882190714598	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.87683789732208E-5	0.0	0.0	0.09919488283153109	0.0
2	4.87683789732208E-5	0.0	0.0	0.36898155531138854	0.0
3	4.87683789732208E-5	0.0	0.0	0.5516679029450736	0.0
4	4.87683789732208E-5	0.0	0.0	0.9128465176207469	0.0
5	4.87683789732208E-5	0.0	0.0	1.6149161013192335	0.0
6	4.87683789732208E-5	0.0	0.0	2.343515683179152	0.0
7	4.87683789732208E-5	0.0	0.0	2.759607492578672	0.0
8	4.87683789732208E-5	0.0	0.0	3.677525921612634	0.0
9	4.87683789732208E-5	0.0	0.0	4.000713969068168	0.0
10	4.87683789732208E-5	0.0	0.0	4.60383251182999	0.0
11	4.87683789732208E-5	0.0	0.0	5.410607805183981	0.0
12	4.87683789732208E-5	0.0	0.0	6.006069712447007	0.0
13	4.87683789732208E-5	0.0	0.0	6.243474181288646	0.0
14	4.87683789732208E-5	0.0	0.0	6.33984049813973	0.0
15	4.87683789732208E-5	0.0	0.0	6.4994106341401086	0.0
16	4.87683789732208E-5	0.0	0.0	6.848055775419664	0.0
17	4.87683789732208E-5	0.0	0.0	7.268731812442667	0.0
18	4.87683789732208E-5	0.0	0.0	7.778068762438985	0.0
19	4.87683789732208E-5	0.0	0.0	8.03766284371344	0.0
20	4.87683789732208E-5	0.0	0.0	8.307303211056377	0.0
21	4.87683789732208E-5	0.0	0.0	8.657313866947183	0.0
22	4.87683789732208E-5	0.0	0.0	9.04112100946643	0.0
23	4.87683789732208E-5	0.0	0.0	9.426196129838981	0.0
24	4.87683789732208E-5	0.0	0.0	9.70554140459759	0.0
25	4.87683789732208E-5	0.0	0.0	9.93992223394289	0.0
26	4.87683789732208E-5	0.0	0.0	10.168938541601134	0.0
27	4.87683789732208E-5	0.0	0.0	10.428191244222775	0.0
28	4.87683789732208E-5	0.0	0.0	10.676373524817496	0.0
29	4.87683789732208E-5	0.0	0.0	10.968154736214277	0.0
30	4.87683789732208E-5	0.0	0.0	11.304705319508473	0.0
31	4.87683789732208E-5	0.0	0.0	11.597559435242664	0.0
32	4.87683789732208E-5	0.0	0.0	11.872125408861898	0.0
33	4.87683789732208E-5	0.0	0.0	12.136254949380861	0.0
34	4.87683789732208E-5	0.0	0.0	12.43574156465541	0.0
35	4.87683789732208E-5	0.0	0.0	12.775754702856705	0.0
36	4.87683789732208E-5	0.0	0.0	13.062073855808485	0.0
37	4.87683789732208E-5	0.0	0.0	13.353708762068345	0.0
38	4.87683789732208E-5	0.0	0.0	13.657974678482269	0.0
39	4.87683789732208E-5	0.0	0.0	14.192524880407742	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCCC	20	7.0345815E-4	45.0	27
CTACGCG	50	2.1827873E-11	45.0	1
CGCGTAG	20	7.0345815E-4	45.0	2
TCGCGAT	20	7.0345815E-4	45.0	1
TACGTAA	20	7.0345815E-4	45.0	24
TTTCGCG	65	0.0	44.999996	1
CTAACGG	30	2.1663382E-6	44.999996	2
CGACGGT	415	0.0	42.831326	28
CGGTCTA	400	0.0	42.75	31
CGTTTTT	4255	0.0	42.09166	1
CGCATCG	135	0.0	41.666664	21
GACGGTC	435	0.0	40.86207	29
ACGGTCT	445	0.0	39.4382	30
TACGACG	80	0.0	39.375	1
TTAACGG	160	0.0	39.375	2
TAGTACG	80	0.0	39.375	1
GGGCGAT	2590	0.0	38.91892	7
AGGGATT	3500	0.0	38.82857	6
TTACCGG	145	0.0	38.793106	2
CTCACGA	460	0.0	38.641304	24
>>END_MODULE