FastQCFastQC Report
Thu 26 May 2016
SRR1547902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547902_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1973056
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128650.652034204807162No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24940.12640289986700834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG351.2125201E-745.09
TCGATAG1050.042.8571431
CGTTTTT65450.040.943471
CGGTCTA2350.040.21276531
GCGATAA1250.039.6000029
CGAGGGA11900.039.516814
CGACGGT2400.039.37499628
AGGGCGA14750.038.593226
ACTCGCG356.250566E-638.5714261
GGGCGAT28800.038.5156257
CACGACG2500.037.80000326
ACGGGAT8650.037.7167635
ACGGGTA2450.037.653065
ATAGGGA25200.037.54
CGGACGG900.037.52
CTCGACC301.1402185E-437.49999636
TACGGGA5250.037.2857174
AATGCGG3450.037.1739122
TAGGGAT25850.037.0793045
TCACGAC2550.037.05882325
Produced by FastQC (version 0.11.5)