##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547902_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1973056
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4642711610821	31.0	31.0	33.0	30.0	34.0
2	31.867094497064453	31.0	31.0	34.0	30.0	34.0
3	31.904328615102663	33.0	31.0	34.0	30.0	34.0
4	35.70822014174965	37.0	35.0	37.0	33.0	37.0
5	35.63649688604885	37.0	35.0	37.0	33.0	37.0
6	35.682506223847675	37.0	35.0	37.0	33.0	37.0
7	36.0217576186383	37.0	35.0	37.0	35.0	37.0
8	36.05026466557462	37.0	35.0	37.0	35.0	37.0
9	37.80354434947614	39.0	38.0	39.0	35.0	39.0
10	37.20959060462552	39.0	37.0	39.0	34.0	39.0
11	37.02056049093386	39.0	37.0	39.0	33.0	39.0
12	36.50670280012326	39.0	35.0	39.0	33.0	39.0
13	36.30741246067015	39.0	35.0	39.0	32.0	39.0
14	37.21048363553797	40.0	35.0	41.0	32.0	41.0
15	37.4235323275163	40.0	35.0	41.0	32.0	41.0
16	37.552635100068116	40.0	35.0	41.0	33.0	41.0
17	37.497633620130394	39.0	35.0	41.0	33.0	41.0
18	37.40463372555062	39.0	35.0	41.0	32.0	41.0
19	37.31875172321516	39.0	35.0	41.0	32.0	41.0
20	37.169547645885366	39.0	35.0	41.0	32.0	41.0
21	37.06906240877096	39.0	35.0	41.0	32.0	41.0
22	37.00796226767005	39.0	35.0	41.0	32.0	41.0
23	36.92225157319407	39.0	35.0	41.0	32.0	41.0
24	36.967631430633496	39.0	35.0	41.0	32.0	41.0
25	36.931739393103896	39.0	35.0	41.0	32.0	41.0
26	36.84248445051737	39.0	35.0	41.0	32.0	41.0
27	36.762131434688115	38.0	35.0	41.0	31.0	41.0
28	36.701379991242014	38.0	35.0	41.0	31.0	41.0
29	36.68173533848	38.0	35.0	41.0	31.0	41.0
30	36.59248850514126	38.0	35.0	41.0	31.0	41.0
31	36.41385191297155	38.0	35.0	41.0	30.0	41.0
32	36.27179309659736	38.0	35.0	41.0	30.0	41.0
33	36.166480829738234	38.0	35.0	41.0	30.0	41.0
34	35.971345466119566	38.0	35.0	41.0	30.0	41.0
35	35.87843477326543	38.0	35.0	41.0	29.0	41.0
36	35.750533689869926	38.0	35.0	40.0	29.0	41.0
37	35.61585580946512	38.0	35.0	40.0	28.0	41.0
38	35.471913113464595	38.0	34.0	40.0	27.0	41.0
39	35.406467429206266	38.0	35.0	40.0	27.0	41.0
40	35.24909429838788	38.0	34.0	40.0	26.0	41.0
41	35.171880575107856	38.0	34.0	40.0	25.0	41.0
42	35.114637394985245	38.0	34.0	40.0	25.0	41.0
43	35.063653033669596	38.0	34.0	40.0	25.0	41.0
44	34.86250719695741	38.0	34.0	40.0	24.0	41.0
45	34.74433670407733	37.0	34.0	40.0	24.0	41.0
46	34.7317922045801	37.0	34.0	40.0	24.0	41.0
47	34.62901661179409	37.0	34.0	40.0	24.0	41.0
48	34.532604244380295	37.0	34.0	40.0	23.0	41.0
49	34.40367683431185	37.0	34.0	40.0	24.0	41.0
50	34.31538841269584	37.0	34.0	40.0	24.0	41.0
51	34.009901898374906	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	5.0
12	6.0
13	10.0
14	25.0
15	62.0
16	147.0
17	384.0
18	832.0
19	1586.0
20	2686.0
21	4499.0
22	6979.0
23	10255.0
24	15173.0
25	21772.0
26	28772.0
27	33165.0
28	36255.0
29	40066.0
30	46852.0
31	56306.0
32	69166.0
33	90497.0
34	152429.0
35	215985.0
36	135070.0
37	178371.0
38	291818.0
39	533820.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.051114616108215	23.960850579000294	28.528536442959552	14.459498361931947
2	30.96120941321483	25.9028633753933	30.10867405689448	13.027253154497389
3	28.898571556002466	25.24388562716922	32.49188061565409	13.365662201174219
4	25.31210467417042	27.584417269454086	31.796563300788215	15.306914755587272
5	23.56750137857212	31.60391798306789	29.883997210418762	14.944583427941224
6	21.288904116254177	40.50422289078465	27.222339355801356	10.984533637159817
7	88.50331668234455	3.3134893282299136	6.613953177203283	1.5692408122222585
8	88.99848762528788	2.516198222452885	6.323084595672905	2.162229556586331
9	84.2721139187129	4.697737925330046	8.316033604722826	2.7141145512342275
10	39.41053877842292	30.200460605274255	20.571337052774986	9.817663563527846
11	34.77093402316001	22.412795176619415	26.245022949171236	16.571247851049336
12	32.58316033604723	21.938657595121477	28.20806910700963	17.270112961821663
13	24.58972274481819	29.29805337506893	29.18563892763307	16.92658495247981
14	18.632365224301793	32.69212835317396	30.75087579876091	17.924630623763342
15	17.66741542054559	26.18263242401635	39.59188183204126	16.5580703233968
16	22.12897150410328	23.615193892114565	36.50225842550845	17.753576178273704
17	21.742515164293362	23.455441710726912	28.770698854974214	26.031344270005512
18	23.948331927730386	24.799650896882806	32.10000121638717	19.152015958999645
19	25.280782704596323	26.919560316585034	28.356772438288623	19.44288454053002
20	26.479532258587696	27.42355006649583	27.57838601641312	18.518531658503356
21	23.739164017645724	26.001745515585974	31.463323899575073	18.79576656719323
22	22.332665671932272	23.267661941678288	31.657084238866002	22.742588147523435
23	19.67090645171754	27.915629358720683	30.441558678516984	21.971905511044795
24	20.874774968373934	27.28331076259366	32.798207450776864	19.043706818255536
25	20.51345729670116	27.168666272016605	30.74813892763307	21.56973750364916
26	19.288707466995362	30.748392341626392	27.757651075286255	22.20524911609199
27	19.230371565733563	29.440725453306953	31.57011255635927	19.758790424600214
28	17.4685868013883	27.65876893509358	34.54286142917383	20.329782834344286
29	19.21891725323559	26.578160984787054	33.584196292451914	20.618725469525447
30	21.487732735411463	26.61602103538876	32.76642933601479	19.12981689318499
31	25.00704490901424	25.832921113237532	29.19587685296312	19.964157124785107
32	26.052783093840215	26.763964124687796	28.346179733367933	18.83707304810406
33	25.363446349216645	26.162359304550908	28.632943008206556	19.841251338025884
34	20.608690275390053	26.89396550325992	31.6043234454572	20.89302077589283
35	20.68344740341886	27.533937201985143	30.53881896915242	21.243796425443577
36	25.96814282007201	25.58518359336988	29.570118638295114	18.876554948263
37	20.567586525673878	28.490524343961855	31.487753008530927	19.454136121833336
38	21.48246172435045	28.5673087839372	28.1937765577865	21.75645293392585
39	20.49926611307535	26.85276038794641	30.063617048882545	22.58435645009569
40	22.902391011709756	25.56491047390444	29.141088747607775	22.391609766778036
41	19.221958221155404	25.275106231146	30.18150523857407	25.321430309124526
42	21.832375766323917	25.95278593207694	29.364245110123584	22.85059319147556
43	22.16039483927471	25.659433893412047	29.738537578254242	22.441633689059003
44	21.446882399688604	27.933216289856954	29.341944678711602	21.27795663174284
45	19.837450128126115	29.528153281001657	27.16233092218366	23.472065668688572
46	22.362011012358494	28.040055629439813	28.95062279022998	20.647310567971715
47	21.06174381264394	27.085901261798956	30.39275114340394	21.459603782153167
48	21.919195400434653	26.091707483213856	29.91623147036881	22.072865645982677
49	21.959792322164194	24.05699584806513	30.96795022543709	23.01526160433358
50	19.714138878977586	27.273376934055598	30.172534383210614	22.839949803756205
51	19.56579032728924	29.156699049596156	28.039295387459862	23.238215235654742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1386.0
1	2277.5
2	3169.0
3	12014.5
4	20860.0
5	15124.0
6	9388.0
7	8662.5
8	7937.0
9	8264.0
10	8591.0
11	8934.0
12	9277.0
13	9119.0
14	8961.0
15	8829.5
16	8698.0
17	8250.0
18	7802.0
19	8001.0
20	8200.0
21	8407.5
22	8615.0
23	10344.5
24	12074.0
25	13638.5
26	19195.0
27	23187.0
28	26286.5
29	29386.0
30	33972.5
31	38559.0
32	42713.5
33	46868.0
34	51500.5
35	56133.0
36	60736.0
37	65339.0
38	73365.5
39	81392.0
40	98540.5
41	115689.0
42	130838.5
43	145988.0
44	152991.0
45	159994.0
46	165686.5
47	171379.0
48	173914.5
49	176450.0
50	173617.0
51	170784.0
52	153341.0
53	135898.0
54	122032.5
55	108167.0
56	99592.0
57	91017.0
58	82282.0
59	73547.0
60	63806.0
61	54065.0
62	46356.5
63	38648.0
64	31899.5
65	25151.0
66	20188.0
67	15225.0
68	11797.5
69	8370.0
70	6881.0
71	5392.0
72	4044.5
73	2697.0
74	2141.5
75	1383.5
76	1181.0
77	824.0
78	467.0
79	325.0
80	183.0
81	122.5
82	62.0
83	47.5
84	33.0
85	25.5
86	18.0
87	14.5
88	11.0
89	10.5
90	10.0
91	8.5
92	7.0
93	5.5
94	4.0
95	5.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1973056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.71371328111646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.81821601838497	17.44826933408464
2	8.20684230734453	3.7281572622471977
3	2.9194030086584575	1.9893144867209083
4	1.5021334336150998	1.364761124844494
5	0.9439754460252393	1.0720593812715655
6	0.6386648080870618	0.8703869600177274
7	0.5063805822363109	0.8051248349228265
8	0.41687900683518525	0.7575096187336788
9	0.3634843214858644	0.7430470794369974
>10	5.352544076958369	32.076568977695764
>50	2.18331706424361	32.860598379669796
>100	0.14250496816396713	4.542178820877766
>500	0.004071569732148155	0.6066204887630597
>1k	0.0013571899107160518	0.4744247982754378
>5k	0.0	0.0
>10k+	2.261983184526753E-4	0.6609784524381099
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12865	0.652034204807162	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2494	0.12640289986700834	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08788397288267541	0.0
2	0.0	0.0	0.0	0.3287286321320834	0.0
3	0.0	0.0	0.0	0.44646477342761687	0.0
4	0.0	0.0	0.0	0.634345908073567	0.0
5	0.0	0.0	0.0	1.0640853579421974	0.0
6	0.0	0.0	0.0	1.537665428654838	0.0
7	0.0	0.0	0.0	1.8255437250640631	0.0
8	0.0	0.0	0.0	2.4986112913166174	0.0
9	0.0	0.0	0.0	2.8389969671413278	0.0
10	0.0	0.0	0.0	3.5300062441207953	0.0
11	0.0	0.0	0.0	4.283101949463168	0.0
12	0.0	0.0	0.0	4.882882188848162	0.0
13	0.0	0.0	0.0	5.127173278406695	0.0
14	0.0	0.0	0.0	5.249420188783288	0.0
15	0.0	0.0	0.0	5.403445213921956	0.0
16	0.0	0.0	0.0	5.699736854909339	0.0
17	0.0	0.0	0.0	6.027907976256122	0.0
18	0.0	0.0	0.0	6.422270832657563	0.0
19	0.0	0.0	0.0	6.631641473936877	0.0
20	0.0	0.0	0.0	6.858548363553797	0.0
21	0.0	0.0	0.0	7.1326409387265235	0.0
22	0.0	0.0	0.0	7.4316187680430765	0.0
23	0.0	0.0	0.0	7.729278844594376	0.0
24	0.0	0.0	0.0	7.961811524862954	0.0
25	0.0	0.0	0.0	8.166468665866555	0.0
26	0.0	0.0	0.0	8.36823688734633	0.0
27	0.0	0.0	0.0	8.614707337247397	0.0
28	0.0	0.0	0.0	8.838775990139155	0.0
29	0.0	0.0	0.0	9.08803399396672	0.0
30	0.0	0.0	0.0	9.413975072172306	0.0
31	0.0	0.0	0.0	9.666375409517013	0.0
32	0.0	0.0	0.0	9.898806724188264	0.0
33	0.0	0.0	0.0	10.138282947873755	0.0
34	0.0	0.0	0.0	10.400211651367218	0.0
35	0.0	0.0	0.0	10.694628028804049	0.0
36	0.0	0.0	0.0	10.947129731746083	0.0
37	0.0	0.0	0.0	11.219448408965585	0.0
38	0.0	0.0	0.0	11.535557024230432	0.0
39	0.0	0.0	0.0	12.069753722144734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	35	1.2125201E-7	45.0	9
TCGATAG	105	0.0	42.857143	1
CGTTTTT	6545	0.0	40.94347	1
CGGTCTA	235	0.0	40.212765	31
GCGATAA	125	0.0	39.600002	9
CGAGGGA	1190	0.0	39.51681	4
CGACGGT	240	0.0	39.374996	28
AGGGCGA	1475	0.0	38.59322	6
ACTCGCG	35	6.250566E-6	38.571426	1
GGGCGAT	2880	0.0	38.515625	7
CACGACG	250	0.0	37.800003	26
ACGGGAT	865	0.0	37.716763	5
ACGGGTA	245	0.0	37.65306	5
ATAGGGA	2520	0.0	37.5	4
CGGACGG	90	0.0	37.5	2
CTCGACC	30	1.1402185E-4	37.499996	36
TACGGGA	525	0.0	37.285717	4
AATGCGG	345	0.0	37.173912	2
TAGGGAT	2585	0.0	37.079304	5
TCACGAC	255	0.0	37.058823	25
>>END_MODULE