FastQCFastQC Report
Thu 26 May 2016
SRR1547901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547901_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1913268
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75540.39482184409084353No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41720.2180562263101667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA302.1662618E-645.00000439
ACGTTGA351.212502E-745.00000421
TTACGTA302.1662618E-645.00000443
CTTTCGT302.1662618E-645.00000420
GTGCGTA351.212502E-745.00000416
GCGTATC302.1662618E-645.00000433
GTTGCGT453.8562575E-1045.00000418
AACACGA302.1662618E-645.00000424
ACGTTGC253.8917813E-545.023
CGACGAA207.034464E-445.016
CGCATTA207.034464E-445.033
ATTACGA253.8917813E-545.010
ATAGCCG207.034464E-445.01
CGTTCAC750.045.021
TAGCCGT253.8917813E-545.044
CGTTACA207.034464E-445.028
TCTACGA207.034464E-445.013
ACTACGC253.8917813E-545.010
CGTGTAC207.034464E-445.021
TAGGCGA551.8189894E-1245.05