##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547901_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1913268
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51280636063531	31.0	31.0	33.0	30.0	34.0
2	31.925371145077428	31.0	31.0	34.0	30.0	34.0
3	32.010687472952036	33.0	31.0	34.0	30.0	34.0
4	35.75751436808643	37.0	35.0	37.0	35.0	37.0
5	35.64177940570793	37.0	35.0	37.0	33.0	37.0
6	35.699842363955284	37.0	35.0	37.0	33.0	37.0
7	36.027586307825146	37.0	35.0	37.0	35.0	37.0
8	36.05432955550398	37.0	35.0	37.0	35.0	37.0
9	37.82086043356184	39.0	38.0	39.0	35.0	39.0
10	37.3588822893604	39.0	37.0	39.0	34.0	39.0
11	37.04272480384348	39.0	37.0	39.0	33.0	39.0
12	36.08483599788425	37.0	35.0	39.0	32.0	39.0
13	35.68381951718212	37.0	35.0	39.0	31.0	39.0
14	36.62232264376972	38.0	35.0	41.0	31.0	41.0
15	36.93878641152207	38.0	35.0	41.0	32.0	41.0
16	37.11461175329332	38.0	35.0	41.0	33.0	41.0
17	37.06582193398938	38.0	35.0	41.0	33.0	41.0
18	37.00230757008428	38.0	35.0	41.0	32.0	41.0
19	36.91703201015226	38.0	35.0	41.0	32.0	41.0
20	36.74981863492203	38.0	35.0	41.0	32.0	41.0
21	36.60504905742426	38.0	35.0	41.0	32.0	41.0
22	36.495395835816	38.0	35.0	40.0	31.0	41.0
23	36.457417361289686	38.0	35.0	40.0	31.0	41.0
24	36.437939692714245	37.0	35.0	40.0	32.0	41.0
25	36.38596631522609	37.0	35.0	40.0	31.0	41.0
26	36.26913950371824	37.0	35.0	40.0	31.0	41.0
27	36.185280891124506	37.0	35.0	40.0	31.0	41.0
28	36.18609624997648	37.0	35.0	40.0	31.0	41.0
29	36.246143770762906	37.0	35.0	40.0	31.0	41.0
30	36.17721511048112	37.0	35.0	40.0	31.0	41.0
31	35.9961641547342	37.0	35.0	40.0	31.0	41.0
32	35.83554055155891	37.0	35.0	40.0	30.0	41.0
33	35.73051240077187	37.0	35.0	40.0	30.0	41.0
34	35.63307126863565	37.0	35.0	40.0	30.0	41.0
35	35.57974784504837	37.0	35.0	40.0	30.0	41.0
36	35.40622066537463	37.0	35.0	40.0	29.0	41.0
37	35.26922417559903	36.0	34.0	40.0	29.0	41.0
38	35.194068996084184	36.0	34.0	40.0	29.0	41.0
39	35.16369060685696	36.0	34.0	40.0	28.0	41.0
40	34.9565575758336	36.0	34.0	40.0	27.0	41.0
41	34.93844040667591	36.0	34.0	40.0	27.0	41.0
42	34.846397368272505	36.0	34.0	40.0	27.0	41.0
43	34.748008120137904	36.0	34.0	40.0	27.0	41.0
44	34.50729171239993	35.0	34.0	40.0	26.0	41.0
45	34.38248431479542	35.0	34.0	40.0	25.0	41.0
46	34.36620536171618	35.0	34.0	40.0	25.0	41.0
47	34.289629576201555	35.0	33.0	40.0	24.0	41.0
48	34.21504985187647	35.0	34.0	40.0	24.0	41.0
49	34.12690537865056	35.0	34.0	40.0	24.0	41.0
50	33.99245009063027	35.0	33.0	39.0	24.0	41.0
51	33.64753291227366	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	2.0
12	3.0
13	6.0
14	19.0
15	63.0
16	120.0
17	300.0
18	686.0
19	1394.0
20	2545.0
21	4035.0
22	6500.0
23	9796.0
24	14263.0
25	20169.0
26	26320.0
27	30559.0
28	34053.0
29	39166.0
30	47163.0
31	57605.0
32	72531.0
33	97687.0
34	189377.0
35	320371.0
36	120533.0
37	148976.0
38	236020.0
39	432913.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.81631219463243	24.280654879504596	30.122439720938203	12.780593204924767
2	29.429071097201227	26.279590731669582	31.247634936663342	13.043703234465845
3	29.026984196672917	24.76615926258109	32.35046005055225	13.856396490193742
4	25.50515662207281	28.448602077701608	30.868179470936642	15.178061829288945
5	23.208144389599365	33.18986153534163	29.29683661672071	14.3051574583383
6	21.902995293915957	40.70062322685583	26.443812367112184	10.952569112116024
7	88.78458219130827	3.811645833202667	5.777496931950987	1.626275043538072
8	89.39479466546244	2.899071118107866	5.087159770612376	2.6189744458173134
9	84.78618782104755	5.242077952487576	7.235891678531184	2.7358425479336925
10	50.904525659761205	27.246365903783477	11.692089137538494	10.157019298916827
11	44.32337759268435	19.75060472448188	21.14319583038027	14.782821852453498
12	41.44761737508807	20.41334512467673	23.53941005651064	14.599627443724557
13	22.17556557680367	39.8346703127842	23.765097205409802	14.224666905002332
14	14.62978526792901	41.83412883087994	26.89053493812681	16.64555096306424
15	13.0873981062768	25.345952579565434	47.36712264042466	14.199526673733109
16	15.477810740575812	20.400592075966355	45.4123520594083	18.70924512404953
17	16.57258679913112	20.592149139587345	27.15463803293632	35.680626028345216
18	20.925348670442407	24.84299115440179	32.90704700021116	21.324613174944652
19	26.477106186901157	26.93924740287299	25.212568234037256	21.371078176188597
20	29.698714450876718	24.533311590430614	26.141972792102308	19.626001166590356
21	21.90519049082512	28.062508754654342	28.74082459958563	21.29147615493491
22	22.38609541371099	24.442524518258814	26.859279515467776	26.312100552562423
23	18.596715149158403	29.512958979087088	25.474632931716833	26.415692940037673
24	20.59889153009406	25.46329108101949	35.88896066834338	18.04885672054307
25	16.852997070980123	26.875220826355743	33.644580895096766	22.627201207567367
26	16.583249184118483	34.129719412021736	26.785792685603898	22.501238718255884
27	17.212695764524362	34.38483265282229	29.103345689155937	19.29912589349741
28	13.978857117769177	29.736816797228617	37.72759487954641	18.556731205455794
29	14.934238172592654	25.26044443329424	37.0247660024628	22.78055139165031
30	17.674575647530823	30.81993740552813	32.21587357338334	19.289613373557703
31	27.234814986713836	28.88084680243437	23.892261826362017	19.992076384489785
32	27.79108833681429	27.879837011856157	26.224083609823612	18.104991041505947
33	25.515975806839396	29.637771603350917	24.666173270028036	20.18007931978165
34	18.486850770514117	29.213837266917132	27.988028859522036	24.311283103046723
35	19.451012612974242	26.94238339845751	29.846210776535226	23.760393212033023
36	28.49004948601032	25.273406548376915	27.042421657603636	19.194122308009124
37	20.799124848165548	29.95957701691556	30.319014377494426	18.92228375742447
38	19.744646332871298	31.272461568374112	24.891755885741045	24.091136213013545
39	19.97942786896556	29.268508123273897	29.210283138588007	21.541780869172538
40	23.329037019382543	26.4981696239105	25.95156559352898	24.22122776317798
41	18.22917646665287	23.394945193250503	28.976912800506778	29.398965539589856
42	22.430887883976524	25.15178218629068	26.415327073886147	26.002002855846644
43	22.389335942481658	26.27540940422356	26.9749977525365	24.360256900758284
44	19.42362491820278	30.70076957331644	27.1819734611147	22.693632047366076
45	17.899740130499232	35.705870792800596	22.90405735108725	23.490331725612933
46	23.56789534973668	31.01551899681592	25.401930100749087	20.014655552698315
47	21.42574903254536	28.0729620732694	27.913810297355102	22.587478596830135
48	23.42567794997878	24.275166887231688	29.782968198914112	22.51618696387542
49	21.941149906860932	22.608385234060258	30.4653085715122	24.985156287566614
50	19.73445434722161	29.585348210496388	28.8259668797053	21.854230562576703
51	17.845173807328614	31.6625271524951	25.012439449151923	25.47985959102436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1426.0
1	1903.5
2	2381.0
3	7123.5
4	11866.0
5	9010.5
6	6155.0
7	6356.0
8	6557.0
9	7033.0
10	7509.0
11	7956.0
12	8403.0
13	8259.5
14	8116.0
15	7750.5
16	7385.0
17	6857.5
18	6330.0
19	5958.5
20	5587.0
21	5870.0
22	6153.0
23	6848.0
24	7543.0
25	9266.0
26	13139.0
27	15289.0
28	17471.5
29	19654.0
30	23972.0
31	28290.0
32	34318.5
33	40347.0
34	45254.5
35	50162.0
36	51931.0
37	53700.0
38	64632.0
39	75564.0
40	105858.0
41	136152.0
42	162278.0
43	188404.0
44	198255.5
45	208107.0
46	200313.0
47	192519.0
48	181687.0
49	170855.0
50	160110.5
51	149366.0
52	131129.5
53	112893.0
54	99647.0
55	86401.0
56	77020.5
57	67640.0
58	65722.5
59	63805.0
60	56287.5
61	48770.0
62	44990.0
63	41210.0
64	34447.5
65	27685.0
66	21524.5
67	15364.0
68	12728.0
69	10092.0
70	8248.0
71	6404.0
72	5070.5
73	3737.0
74	3135.5
75	1835.5
76	1137.0
77	806.0
78	475.0
79	308.0
80	141.0
81	108.5
82	76.0
83	58.0
84	40.0
85	31.5
86	23.0
87	19.5
88	16.0
89	13.0
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1913268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.367359455705866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92417879530453	14.312614022240513
2	9.407556413494293	3.455839404929615
3	3.279666581469381	1.8071644499014248
4	1.6597292060627968	1.219393717075548
5	0.9646812139460738	0.8859323308357114
6	0.5798998215183779	0.6390737082076634
7	0.3890773955733027	0.50024270664093
8	0.3000649838688146	0.440912113503128
9	0.22131052735552076	0.3658401006543608
>10	2.1199460832063615	9.070986758276115
>50	1.4181576779594105	19.760245355250323
>100	1.7245126193150373	45.40435740091239
>500	0.008917413043902683	1.0806397363535982
>1k	0.002013609397010283	0.6576405807653273
>5k	2.876584852871833E-4	0.3991176144533849
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7554	0.39482184409084353	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4172	0.2180562263101667	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09052573920642587	0.0
2	0.0	0.0	0.0	0.3602213594749925	0.0
3	0.0	0.0	0.0	0.5146168754194395	0.0
4	0.0	0.0	0.0	0.9135677803632319	0.0
5	0.0	0.0	0.0	1.4869323063993125	0.0
6	0.0	0.0	0.0	2.126518605861803	0.0
7	0.0	0.0	0.0	2.4440381587942723	0.0
8	0.0	0.0	0.0	3.2181586688325945	0.0
9	0.0	0.0	0.0	3.468097516918696	0.0
10	0.0	0.0	0.0	3.9689682783593305	0.0
11	0.0	0.0	0.0	4.638973734991648	0.0
12	0.0	0.0	0.0	5.160071667952425	0.0
13	0.0	0.0	0.0	5.359677786907009	0.0
14	0.0	0.0	0.0	5.438234476299191	0.0
15	0.0	0.0	0.0	5.572089221165044	0.0
16	0.0	0.0	0.0	5.892797036275106	0.0
17	0.0	0.0	0.0	6.277061028564739	0.0
18	0.0	0.0	0.0	6.813211740331203	0.0
19	0.0	0.0	0.0	7.040675953394924	0.0
20	0.0	0.0	0.0	7.276607354536845	0.0
21	0.0	0.0	0.0	7.597001570088456	0.0
22	0.0	0.0	0.0	7.9077787325142115	0.0
23	0.0	0.0	0.0	8.265073162776988	0.0
24	0.0	0.0	0.0	8.492014709909956	0.0
25	0.0	0.0	0.0	8.685244304509352	0.0
26	0.0	0.0	0.0	8.885791222139293	0.0
27	0.0	0.0	0.0	9.07133762755662	0.0
28	0.0	0.0	0.0	9.260176828337693	0.0
29	0.0	0.0	0.0	9.48032371837087	0.0
30	0.0	0.0	0.0	9.768939845332698	0.0
31	0.0	0.0	0.0	10.031422675756872	0.0
32	0.0	0.0	0.0	10.24555890758639	0.0
33	0.0	0.0	0.0	10.469834858472518	0.0
34	0.0	0.0	0.0	10.708013723116677	0.0
35	0.0	0.0	0.0	11.017797820274003	0.0
36	0.0	0.0	0.0	11.262039609714897	0.0
37	5.2266593075303614E-5	0.0	0.0	11.506856331679618	0.0
38	5.2266593075303614E-5	0.0	0.0	11.745244262696078	0.0
39	5.2266593075303614E-5	0.0	0.0	12.067781408563777	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTA	30	2.1662618E-6	45.000004	39
ACGTTGA	35	1.212502E-7	45.000004	21
TTACGTA	30	2.1662618E-6	45.000004	43
CTTTCGT	30	2.1662618E-6	45.000004	20
GTGCGTA	35	1.212502E-7	45.000004	16
GCGTATC	30	2.1662618E-6	45.000004	33
GTTGCGT	45	3.8562575E-10	45.000004	18
AACACGA	30	2.1662618E-6	45.000004	24
ACGTTGC	25	3.8917813E-5	45.0	23
CGACGAA	20	7.034464E-4	45.0	16
CGCATTA	20	7.034464E-4	45.0	33
ATTACGA	25	3.8917813E-5	45.0	10
ATAGCCG	20	7.034464E-4	45.0	1
CGTTCAC	75	0.0	45.0	21
TAGCCGT	25	3.8917813E-5	45.0	44
CGTTACA	20	7.034464E-4	45.0	28
TCTACGA	20	7.034464E-4	45.0	13
ACTACGC	25	3.8917813E-5	45.0	10
CGTGTAC	20	7.034464E-4	45.0	21
TAGGCGA	55	1.8189894E-12	45.0	5
>>END_MODULE