FastQCFastQC Report
Thu 26 May 2016
SRR1547900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547900_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4123921
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400680.9715996014472634No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43850.10633084387407032No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT219750.043.2081871
CGGTCTA4850.042.68041231
TACGGGT7350.039.4897964
TACGCGG2850.039.4736862
TACGGGA14350.038.8850174
TCACGAC5500.038.86363625
AGGGCGA30500.038.655746
CGCGTAG356.252225E-638.5714262
GGGCGAT59200.038.196797
TAGGGCG12550.037.8286865
CGGTTTT23150.037.6133961
CGACGGT5450.037.5688128
GGCGATA12650.037.5296488
TAGGGAT57800.036.864195
CGGGTAT4900.036.7346956
ATAGGGA56450.036.7094764
TAAGGGA52650.036.5811964
GCGATCG1600.036.56259
CCGCTCG2350.036.3829819
AGGGATT63850.036.2960056
Produced by FastQC (version 0.11.5)