FastQCFastQC Report
Thu 26 May 2016
SRR1547899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547899_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1911984
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372071.945989087774793No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.2075854191248462No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC24160.12636089004928913No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTT22460.11746960225608581No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA253.89178E-545.00000443
CGTTTTT162150.043.6956521
CTATGCG750.042.01
CGGTTTT15050.041.8604661
ATAACGC650.041.53846411
AACGGGA8700.040.3448264
TACGTAG950.040.2631571
CGCGAGG1750.039.8571432
TACGGGA8200.039.7865874
CGGTCTA2000.039.37500431
TAGGGAC22450.039.2873085
TTACGAG1150.039.1304321
CACGACC5600.038.97321327
CGAACGG1100.038.8636362
CGGGTAT1450.038.7931026
TAAGGGA27150.038.3701674
GCGAGAC5750.038.34782821
CGAGACA5700.038.28947422
TAGGGAT32100.038.130845
GTTAGCG1300.038.0769271
Produced by FastQC (version 0.11.5)