##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547899_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1911984
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4784443802877	31.0	31.0	33.0	30.0	34.0
2	31.84619850375317	31.0	31.0	34.0	30.0	34.0
3	31.59635750089959	31.0	31.0	34.0	30.0	34.0
4	35.552198658566184	37.0	35.0	37.0	33.0	37.0
5	35.59275862141106	37.0	35.0	37.0	33.0	37.0
6	35.68350310462849	37.0	35.0	37.0	33.0	37.0
7	36.03486430848794	37.0	35.0	37.0	35.0	37.0
8	36.078072829061334	37.0	35.0	37.0	35.0	37.0
9	37.84703219273801	39.0	38.0	39.0	35.0	39.0
10	37.385519439493216	39.0	37.0	39.0	35.0	39.0
11	37.134063360362845	39.0	37.0	39.0	33.0	39.0
12	36.788947501652736	39.0	35.0	39.0	33.0	39.0
13	36.68698012117256	39.0	35.0	39.0	33.0	39.0
14	37.75728667185499	40.0	36.0	41.0	33.0	41.0
15	37.909700081172225	40.0	36.0	41.0	33.0	41.0
16	37.97749771964619	40.0	36.0	41.0	33.0	41.0
17	37.889077523661285	40.0	36.0	41.0	33.0	41.0
18	37.707264809747365	39.0	36.0	41.0	33.0	41.0
19	37.53063310153223	39.0	36.0	41.0	33.0	41.0
20	37.290444376103565	39.0	35.0	41.0	32.0	41.0
21	37.18468146176956	39.0	35.0	41.0	32.0	41.0
22	37.115923041197	39.0	35.0	41.0	32.0	41.0
23	37.025783688566435	39.0	35.0	41.0	32.0	41.0
24	37.030621072142864	39.0	35.0	41.0	32.0	41.0
25	36.99370601427627	39.0	35.0	41.0	32.0	41.0
26	36.90547253533502	39.0	35.0	41.0	32.0	41.0
27	36.83649078653378	39.0	35.0	41.0	32.0	41.0
28	36.75202564456606	38.0	35.0	41.0	31.0	41.0
29	36.709575498539735	38.0	35.0	41.0	31.0	41.0
30	36.58085109498824	38.0	35.0	41.0	31.0	41.0
31	36.36314163716851	38.0	35.0	41.0	31.0	41.0
32	36.10143547226337	38.0	35.0	41.0	30.0	41.0
33	35.84467861655746	38.0	35.0	41.0	29.0	41.0
34	35.53135486489427	38.0	35.0	41.0	26.0	41.0
35	35.30651616331517	38.0	35.0	41.0	25.0	41.0
36	35.15009905940636	38.0	34.0	41.0	24.0	41.0
37	35.015297199139745	38.0	34.0	40.0	23.0	41.0
38	34.85037322488054	38.0	34.0	40.0	23.0	41.0
39	34.74214428572624	38.0	34.0	40.0	23.0	41.0
40	34.59907823496431	38.0	34.0	40.0	22.0	41.0
41	34.50801418840325	38.0	34.0	40.0	21.0	41.0
42	34.443259985439205	38.0	34.0	40.0	21.0	41.0
43	34.3936262018929	38.0	34.0	40.0	21.0	41.0
44	34.21333442120854	38.0	33.0	40.0	20.0	41.0
45	34.09200024686399	37.0	33.0	40.0	20.0	41.0
46	34.061672064201375	37.0	33.0	40.0	20.0	41.0
47	33.98312642783622	37.0	33.0	40.0	20.0	41.0
48	33.8810068494297	37.0	33.0	40.0	20.0	41.0
49	33.74885093180696	37.0	33.0	40.0	19.0	41.0
50	33.684067439894896	37.0	33.0	40.0	19.0	41.0
51	33.36927714876275	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	5.0
13	7.0
14	21.0
15	48.0
16	144.0
17	341.0
18	808.0
19	1574.0
20	2824.0
21	4570.0
22	6987.0
23	11058.0
24	18013.0
25	28704.0
26	39751.0
27	43310.0
28	42394.0
29	42117.0
30	45620.0
31	52130.0
32	62737.0
33	79738.0
34	129460.0
35	181341.0
36	136999.0
37	178175.0
38	281772.0
39	521260.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.487424580958837	23.982052151064025	28.15635486489427	19.37416840308287
2	36.00500841011222	23.400091214152418	27.694374011498002	12.900526364237358
3	27.16079214052001	24.292096586582314	35.92012276253358	12.626988510364104
4	23.94413342371066	26.86256788759739	35.53105046904158	13.662248219650374
5	21.78653168645763	30.447378220738248	34.28323667980485	13.48285341299927
6	19.878513627729102	38.42223575092679	31.173168813128143	10.526081808215968
7	83.60598205842726	2.7102737261399676	12.050571552900024	1.633172662532741
8	84.49647068176303	1.9895040962685882	11.724470497661068	1.7895547243073164
9	79.86400513811832	4.512433158436472	13.07165750341007	2.5519042000351466
10	35.593812500523015	36.49627821153315	18.1289173967983	9.780991891145533
11	27.34698616724826	23.708252788726266	32.72020058745262	16.22456045657286
12	26.6969284261793	22.302853998778232	33.62899480330379	17.371222771738676
13	22.351703779947947	26.047184495267743	34.43538230445443	17.16572942032988
14	17.02169055807998	29.837278973045798	35.050502514665396	18.09052795420882
15	16.491665202219266	26.586414949079074	40.30572431568465	16.616195533017013
16	21.034956359467444	25.02076377208177	37.713966225658794	16.23031364279199
17	20.151110051130136	24.83749864015598	33.28652331818676	21.724867990527116
18	21.645055607159893	25.70230713227726	34.86477920317325	17.787858057389602
19	22.273251240596156	27.990976911940685	31.65183390655989	18.083937940903272
20	23.654591251809638	27.54421585117867	31.209256981229966	17.591935915781722
21	21.748874467568765	26.862358680825782	34.60923313165801	16.779533719947448
22	20.976012351567796	23.69407902995004	34.667183407392535	20.662725211089633
23	18.32965129415309	27.902639352630565	34.07329768449945	19.694411668716892
24	18.773274253341032	27.285897789939668	36.19094092837597	17.749887028343334
25	20.071925288077725	27.897356881647546	33.32904459451543	18.701673235759294
26	18.639434221206873	29.527339140913313	31.54780583937941	20.285420798500407
27	17.995652683286053	28.207819730709044	34.32868685093599	19.46784073506891
28	16.636488589862676	28.272464623134923	35.96348086594867	19.127565921053733
29	17.67049305851932	27.15482974752927	35.573937857220564	19.60073933673085
30	19.690279834977698	26.42987598222579	35.53654214679621	18.3433020360003
31	22.05039372714416	26.726374279282673	31.72641612063699	19.496815872936175
32	21.424760876660056	27.645524230328288	32.35157825588499	18.578136637126672
33	20.62365584649244	27.121095155608	31.96365659963682	20.291592398262747
34	17.673055841471477	28.094272755420548	33.864613929823676	20.368057473284296
35	18.021175909421835	28.530521175909424	33.4627800232638	19.98552289140495
36	22.153271157080813	28.274347484079364	30.747171524447904	18.82520983439192
37	19.989393216679638	29.11964744474849	31.624689327944168	19.266270010627704
38	20.012092151398754	29.867980066778806	30.128913212662866	19.991014569159574
39	20.073651243943463	28.15635486489427	30.239740499920504	21.530253391241768
40	22.057611360764525	27.143689486941312	30.650988711202604	20.147710441091558
41	19.013495928836225	27.095205817621903	31.063387559728532	22.82791069381334
42	20.64557025581804	28.2145143474004	29.83539611210136	21.304519284680207
43	20.77282027464665	27.98062117674625	29.9814747403744	21.265083808232703
44	20.578310278747104	29.326082226629513	29.68612708056134	20.409480414062042
45	19.46825914861212	29.533196930518248	28.908400907120562	22.09014301374907
46	20.928313207641907	28.597728851287457	29.49658574548741	20.97737219558323
47	19.82537510774149	28.55034351751897	31.287134201959848	20.337147172779687
48	20.290127950861514	28.084910752391234	30.843511242771903	20.781450053975348
49	20.637986510347368	26.9200474480958	30.590894066059132	21.851071975497703
50	18.798169859162	28.417026502313824	31.78499401668633	20.99980962183784
51	18.401252311734826	28.734079364680877	30.719242420438665	22.145425903145632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1174.0
1	1821.0
2	2468.0
3	28568.5
4	54669.0
5	38388.5
6	22108.0
7	19429.0
8	16750.0
9	17489.0
10	18228.0
11	18891.5
12	19555.0
13	19484.0
14	19413.0
15	18543.5
16	17674.0
17	16697.5
18	15721.0
19	15498.5
20	15276.0
21	15147.0
22	15018.0
23	15587.5
24	16157.0
25	17528.0
26	21223.0
27	23547.0
28	26995.0
29	30443.0
30	35218.5
31	39994.0
32	44459.5
33	48925.0
34	55649.0
35	62373.0
36	67993.5
37	73614.0
38	79398.0
39	85182.0
40	94240.0
41	103298.0
42	114634.5
43	125971.0
44	131008.0
45	136045.0
46	144372.5
47	152700.0
48	155967.0
49	159234.0
50	150149.0
51	141064.0
52	128872.0
53	116680.0
54	104178.5
55	91677.0
56	82837.5
57	73998.0
58	67908.0
59	61818.0
60	52988.0
61	44158.0
62	38698.5
63	33239.0
64	27678.0
65	22117.0
66	17709.0
67	13301.0
68	10380.5
69	7460.0
70	6024.0
71	4588.0
72	3774.0
73	2960.0
74	2513.5
75	1651.0
76	1235.0
77	983.0
78	731.0
79	495.0
80	259.0
81	183.0
82	107.0
83	79.0
84	51.0
85	37.0
86	23.0
87	15.5
88	8.0
89	6.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1911984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.745832650094666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67258628721038	20.441072500245983
2	7.94025074487177	4.406177367340033
3	3.3092597831578896	2.7545450441756216
4	2.005846142103219	2.2261548552253574
5	1.3550735072705347	1.8798821380652548
6	1.0259925262242902	1.708021015972021
7	0.8295129379928425	1.6110869011046438
8	0.7102843658759596	1.5765944919658388
9	0.6380945122605055	1.5934017196911388
>10	8.043560460932571	49.40931555364288
>50	0.42975155808722765	7.294002682251491
>100	0.035196383216493606	1.7304564048912552
>500	0.0026779612978465967	0.5407612774366916
>1k	0.0017215465486156694	0.8538391825892132
>5k	0.0	0.0
>10k+	1.912829498461855E-4	1.974688865402589
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37207	1.945989087774793	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3969	0.2075854191248462	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC	2416	0.12636089004928913	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTT	2246	0.11746960225608581	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.2301692901195824E-5	0.0	0.0	0.06658005506322229	0.0
2	5.2301692901195824E-5	0.0	0.0	0.1994263550322597	0.0
3	5.2301692901195824E-5	0.0	0.0	0.38253458187934625	0.0
4	5.2301692901195824E-5	0.0	0.0	0.5259981255073264	0.0
5	5.2301692901195824E-5	0.0	0.0	0.9778847521736583	0.0
6	5.2301692901195824E-5	0.0	0.0	1.710369961254906	0.0
7	5.2301692901195824E-5	0.0	0.0	2.1736583569736987	0.0
8	5.2301692901195824E-5	0.0	0.0	2.9324513175842477	0.0
9	5.2301692901195824E-5	0.0	0.0	3.4326647084912842	0.0
10	5.2301692901195824E-5	0.0	0.0	4.029531627879731	0.0
11	5.2301692901195824E-5	0.0	0.0	4.569808115549084	0.0
12	5.2301692901195824E-5	0.0	0.0	4.951244361877505	0.0
13	5.2301692901195824E-5	0.0	0.0	5.163484631670558	0.0
14	5.2301692901195824E-5	0.0	0.0	5.302711738173541	0.0
15	5.2301692901195824E-5	0.0	0.0	5.427137465585486	0.0
16	5.2301692901195824E-5	0.0	0.0	5.618561661603863	0.0
17	5.2301692901195824E-5	0.0	0.0	5.849735144227148	0.0
18	5.2301692901195824E-5	0.0	0.0	6.142520021088043	0.0
19	5.2301692901195824E-5	0.0	0.0	6.316109339827111	0.0
20	5.2301692901195824E-5	0.0	0.0	6.499479075138704	0.0
21	5.2301692901195824E-5	0.0	0.0	6.709208863672499	0.0
22	5.2301692901195824E-5	0.0	0.0	6.940068536138377	0.0
23	5.2301692901195824E-5	0.0	0.0	7.190018326513193	3.1381015740717495E-4
24	5.2301692901195824E-5	0.0	0.0	7.376160051548548	3.1381015740717495E-4
25	5.2301692901195824E-5	0.0	0.0	7.537510774148737	3.1381015740717495E-4
26	5.2301692901195824E-5	0.0	0.0	7.693579025765906	3.1381015740717495E-4
27	5.2301692901195824E-5	0.0	0.0	7.881969723596013	3.1381015740717495E-4
28	5.2301692901195824E-5	0.0	0.0	8.062149055640633	3.1381015740717495E-4
29	5.2301692901195824E-5	0.0	0.0	8.276167582992326	3.1381015740717495E-4
30	5.2301692901195824E-5	0.0	0.0	8.533282705294605	3.1381015740717495E-4
31	5.2301692901195824E-5	0.0	0.0	8.734225809421	3.1381015740717495E-4
32	5.2301692901195824E-5	0.0	0.0	8.94066059130202	3.1381015740717495E-4
33	5.2301692901195824E-5	0.0	0.0	9.139563929405266	3.1381015740717495E-4
34	5.2301692901195824E-5	0.0	0.0	9.347567762073323	3.1381015740717495E-4
35	5.2301692901195824E-5	0.0	0.0	9.577172193909572	3.1381015740717495E-4
36	5.2301692901195824E-5	0.0	0.0	9.777592281106955	3.1381015740717495E-4
37	5.2301692901195824E-5	0.0	0.0	9.987845086569761	3.1381015740717495E-4
38	5.2301692901195824E-5	0.0	0.0	10.240357659896736	3.1381015740717495E-4
39	1.0460338580239165E-4	0.0	0.0	10.624879706106327	3.1381015740717495E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	25	3.89178E-5	45.000004	43
CGTTTTT	16215	0.0	43.695652	1
CTATGCG	75	0.0	42.0	1
CGGTTTT	1505	0.0	41.860466	1
ATAACGC	65	0.0	41.538464	11
AACGGGA	870	0.0	40.344826	4
TACGTAG	95	0.0	40.263157	1
CGCGAGG	175	0.0	39.857143	2
TACGGGA	820	0.0	39.786587	4
CGGTCTA	200	0.0	39.375004	31
TAGGGAC	2245	0.0	39.287308	5
TTACGAG	115	0.0	39.130432	1
CACGACC	560	0.0	38.973213	27
CGAACGG	110	0.0	38.863636	2
CGGGTAT	145	0.0	38.793102	6
TAAGGGA	2715	0.0	38.370167	4
GCGAGAC	575	0.0	38.347828	21
CGAGACA	570	0.0	38.289474	22
TAGGGAT	3210	0.0	38.13084	5
GTTAGCG	130	0.0	38.076927	1
>>END_MODULE