FastQCFastQC Report
Thu 26 May 2016
SRR1547898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547898_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2541772
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410211.6138741004307229No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40360.15878686207889614No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT207.0349016E-445.023
CGTTTTT153150.043.486781
TCGATAG1100.042.9545481
CGGTCTA3600.040.62531
TTATACG950.040.2631571
TACGTAG2100.039.642861
CGGTTTT15050.039.468441
AGGGCGA17200.039.3756
CGAGGGA17300.039.277464
TGCGTAG2150.038.7209321
GTCGCGC356.251279E-638.57142639
TAACGGG7150.038.3916133
TACGGGA10200.038.3823554
CGTTAGG4600.038.1521762
AAGGGAT42400.038.048355
CGACGGT3850.037.98701528
CTAACGG1900.037.8947372
GGGCGAT35300.037.8611877
ACGGGAT10900.037.568815
ACGGCGC301.1403108E-437.50000426
Produced by FastQC (version 0.11.5)