##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547898_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2541772
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477350053427294	31.0	31.0	33.0	30.0	34.0
2	31.862769359328848	31.0	31.0	34.0	30.0	34.0
3	31.758855239573023	33.0	31.0	34.0	30.0	34.0
4	35.627666446872496	37.0	35.0	37.0	33.0	37.0
5	35.609276127048375	37.0	35.0	37.0	33.0	37.0
6	35.680193974911994	37.0	35.0	37.0	33.0	37.0
7	36.01767861161426	37.0	35.0	37.0	35.0	37.0
8	36.0577726877155	37.0	35.0	37.0	35.0	37.0
9	37.8170996454442	39.0	38.0	39.0	35.0	39.0
10	37.37150814471164	39.0	37.0	39.0	35.0	39.0
11	37.06316026771874	39.0	37.0	39.0	33.0	39.0
12	36.55490657698645	39.0	35.0	39.0	33.0	39.0
13	36.37540345868945	39.0	35.0	39.0	32.0	39.0
14	37.33178467620227	40.0	35.0	41.0	32.0	41.0
15	37.55362322033605	40.0	35.0	41.0	32.0	41.0
16	37.663868356406475	40.0	35.0	41.0	33.0	41.0
17	37.62552738797972	40.0	35.0	41.0	33.0	41.0
18	37.48310273305395	39.0	36.0	41.0	33.0	41.0
19	37.3668287320814	39.0	35.0	41.0	32.0	41.0
20	37.15113235962942	39.0	35.0	41.0	32.0	41.0
21	37.057221497443514	39.0	35.0	41.0	32.0	41.0
22	36.99692812730646	39.0	35.0	41.0	32.0	41.0
23	36.921088909626825	38.0	35.0	41.0	32.0	41.0
24	36.91204915311051	38.0	35.0	41.0	32.0	41.0
25	36.85479736184048	38.0	35.0	41.0	32.0	41.0
26	36.7477295367169	38.0	35.0	41.0	32.0	41.0
27	36.66844075707813	38.0	35.0	41.0	31.0	41.0
28	36.595855568477425	38.0	35.0	41.0	31.0	41.0
29	36.57873522880888	38.0	35.0	41.0	31.0	41.0
30	36.47142780705744	38.0	35.0	40.0	31.0	41.0
31	36.25905352643746	38.0	35.0	40.0	30.0	41.0
32	36.045362054503705	38.0	35.0	40.0	30.0	41.0
33	35.866076107534425	38.0	35.0	40.0	30.0	41.0
34	35.63486614849798	38.0	35.0	40.0	29.0	41.0
35	35.4866577332664	38.0	35.0	40.0	27.0	41.0
36	35.34492236125034	38.0	35.0	40.0	26.0	41.0
37	35.22846148277658	38.0	34.0	40.0	26.0	41.0
38	35.0900155482081	38.0	34.0	40.0	25.0	41.0
39	35.009295090196915	38.0	34.0	40.0	24.0	41.0
40	34.873199091027836	38.0	34.0	40.0	24.0	41.0
41	34.790429668750775	38.0	34.0	40.0	23.0	41.0
42	34.73247403779725	37.0	34.0	40.0	23.0	41.0
43	34.663441488851085	37.0	34.0	40.0	23.0	41.0
44	34.47726389306358	37.0	34.0	40.0	23.0	41.0
45	34.34740055362951	37.0	34.0	40.0	23.0	41.0
46	34.338210114833274	37.0	34.0	40.0	23.0	41.0
47	34.2626856382083	37.0	33.0	40.0	23.0	41.0
48	34.163210547602226	36.0	33.0	40.0	23.0	41.0
49	34.031336406255164	36.0	33.0	40.0	23.0	41.0
50	33.96112869289614	36.0	33.0	40.0	22.0	41.0
51	33.67599965693225	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	7.0
12	4.0
13	7.0
14	35.0
15	79.0
16	192.0
17	486.0
18	1089.0
19	2101.0
20	3765.0
21	6004.0
22	9261.0
23	14235.0
24	21338.0
25	32751.0
26	44060.0
27	49972.0
28	51075.0
29	54262.0
30	60891.0
31	71567.0
32	87674.0
33	114747.0
34	194803.0
35	282217.0
36	187405.0
37	227283.0
38	356893.0
39	667463.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.332314621453065	23.939912785253753	29.062402135203314	15.665370458089868
2	33.3901309794899	25.13577142245646	28.865885689196357	12.608211908857287
3	28.029146595367326	24.755957654738506	33.99112902337424	13.223766726519923
4	24.646624480873975	26.82691445180764	33.98998808705108	14.536472980267309
5	22.993879860192024	31.40608205614036	32.23038887831009	13.369649205357522
6	20.74017653825756	39.95496055507732	28.962865276665255	10.341997629999858
7	86.06165305149321	3.3111152377160504	9.079925343421834	1.5473063673689063
8	87.12040261675713	2.402379127632219	8.765459687178865	1.711758568431787
9	82.37784506242102	5.2173837779313015	10.02080438371341	2.3839667759342693
10	42.6515438835584	33.478573215850986	14.678539223817086	9.191343676773526
11	31.97902880352762	23.108484946722207	29.117717875560828	15.794768374189344
12	31.20091023113009	22.19526377660939	30.27187332302032	16.3319526692402
13	24.283964100635306	28.88866507302779	30.47673040697592	16.350640419360982
14	17.168180308855398	32.59910802385107	32.875371984583985	17.357339682709544
15	16.280374478906843	26.97244284695874	40.895682224841565	15.851500449292855
16	21.364111336500677	24.606770394826917	37.77042157990567	16.258696688766737
17	21.41891562264436	24.32444766879169	30.094083969765972	24.16255273879797
18	22.679414203949054	25.772138492358877	33.18067080760981	18.36777649608226
19	25.0602729119685	27.38994685597292	29.265528143358253	18.284252088700324
20	24.94507768596082	28.361827890149076	28.887799535127463	17.805294888762642
21	22.711989903106968	27.56100861918378	32.23385102991141	17.493150447797838
22	22.073104904767227	24.250444178313398	31.95664284601451	21.719808070904865
23	19.083340283864956	28.250763640483882	31.468440127596025	21.197455948055136
24	19.56221879853897	28.319731274087527	34.29446071480841	17.823589212565093
25	20.306502707559922	28.149967817727163	32.08497851105449	19.458550963658425
26	18.390241138859032	31.537604474358833	29.044658608246532	21.027495778535606
27	17.844401464804868	30.287334977330776	32.709582134038776	19.158681423825584
28	16.92445270464857	28.624243244476688	35.16782779887417	19.283476252000572
29	18.213317323505017	27.75579398939008	34.28564796527777	19.745240721827134
30	20.764962396312495	27.90671232510233	32.813682737869485	18.51464254071569
31	24.793254469716402	27.082798929250934	29.539274175653834	18.58467242537883
32	25.242114556301665	28.57655997469482	28.802819450367696	17.378506018635818
33	24.183286305774082	28.63553457981282	28.281962347527628	18.899216766885466
34	19.694134643075774	28.748920044756176	31.20913284118324	20.34781247098481
35	19.785842317879023	29.918576489157957	30.415552614475256	19.880028578487764
36	24.408719586178464	28.56522929672685	28.949370753946457	18.076680363148228
37	20.645124739748493	31.497829073575446	29.476837418934508	18.38020876774156
38	20.31354503865807	31.67644462209828	27.804775566022442	20.2052347732212
39	19.468071880562064	29.981721413250284	28.985054521019194	21.565152185168458
40	22.49950034857572	28.38865956505934	28.225033559264958	20.886806527099992
41	18.606782984469103	27.694026057411914	29.463854350429543	24.23533660768944
42	20.909861309354262	28.32075418251519	28.70155151602897	22.067832992101575
43	20.835385707293966	28.172235747344764	28.89964166730926	22.092736878052005
44	20.677464383115403	29.964686053666494	29.24042754424866	20.117422018969442
45	19.343985219760075	31.246587026688466	27.19889903579078	22.21052871776068
46	21.368281655475	29.65702667273068	28.73160928674956	20.243082385044765
47	20.446798532677203	28.986352827869695	30.029011256713822	20.537837382739283
48	21.152566005133426	28.296165037619424	29.89918057166418	20.65208838558297
49	20.901245272982784	26.94269981729282	30.472166661683264	21.683888248041132
50	19.01822822818097	29.568191010051255	30.072720920680535	21.34085984108724
51	18.94123469768335	31.058607931789318	28.366942432287395	21.633214938239938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1614.0
1	2686.5
2	3759.0
3	29959.0
4	56159.0
5	38573.5
6	20988.0
7	19159.0
8	17330.0
9	18782.5
10	20235.0
11	21504.0
12	22773.0
13	22874.5
14	22976.0
15	21777.5
16	20579.0
17	19030.5
18	17482.0
19	17041.0
20	16600.0
21	16586.0
22	16572.0
23	16529.5
24	16487.0
25	19052.0
26	23658.0
27	25699.0
28	30882.5
29	36066.0
30	43922.0
31	51778.0
32	58579.5
33	65381.0
34	71167.0
35	76953.0
36	84716.0
37	92479.0
38	102944.0
39	113409.0
40	128116.5
41	142824.0
42	160327.0
43	177830.0
44	186436.0
45	195042.0
46	201248.5
47	207455.0
48	208644.5
49	209834.0
50	201518.5
51	193203.0
52	176527.0
53	159851.0
54	143025.0
55	126199.0
56	116475.5
57	106752.0
58	96667.5
59	86583.0
60	77502.0
61	68421.0
62	58717.5
63	49014.0
64	40805.5
65	32597.0
66	25979.5
67	19362.0
68	15859.5
69	12357.0
70	9925.5
71	7494.0
72	6129.5
73	4765.0
74	3690.5
75	1972.5
76	1329.0
77	922.5
78	516.0
79	443.0
80	370.0
81	292.0
82	214.0
83	161.5
84	109.0
85	75.0
86	41.0
87	28.0
88	15.0
89	16.5
90	18.0
91	13.5
92	9.0
93	8.0
94	7.0
95	7.0
96	7.0
97	3.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2541772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.140199297395984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15785333014841	16.861500507913387
2	8.404872702585722	3.7217111340898223
3	3.1360317072564863	2.0829710100483476
4	1.7752472357828686	1.572173104095361
5	1.0997869588329001	1.2174751226618723
6	0.7954714485248183	1.0567137843436651
7	0.5450410267549621	0.844712187032854
8	0.4491981654149187	0.7956269525048758
9	0.39937487733501914	0.7958015440713361
>10	4.775368188718672	26.406818311141418
>50	2.1757405902875506	33.823257154325695
>100	0.28057978080410834	8.007679644267776
>500	0.0036226583689464766	0.5782478841725632
>1k	0.0016301962660259144	0.590238737724654
>5k	0.0	0.0
>10k+	1.811329184473238E-4	1.6450729216064026
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41021	1.6138741004307229	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4036	0.15878686207889614	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5737052733290005E-4	0.0	0.0	0.05319123823852021	0.0
2	1.5737052733290005E-4	0.0	0.0	0.15351494941324398	0.0
3	1.5737052733290005E-4	0.0	0.0	0.2626907527504434	0.0
4	1.5737052733290005E-4	0.0	0.0	0.389216656726095	0.0
5	1.5737052733290005E-4	0.0	0.0	0.7086788272118821	0.0
6	1.5737052733290005E-4	0.0	0.0	1.1924358282332168	0.0
7	1.5737052733290005E-4	0.0	0.0	1.5242122424828033	0.0
8	1.5737052733290005E-4	0.0	0.0	2.1419309048962694	0.0
9	1.5737052733290005E-4	0.0	0.0	2.515017082570742	0.0
10	1.5737052733290005E-4	0.0	0.0	3.014275080534367	0.0
11	1.5737052733290005E-4	0.0	0.0	3.515146126403155	0.0
12	1.5737052733290005E-4	0.0	0.0	3.8836292161531403	0.0
13	1.5737052733290005E-4	0.0	0.0	4.048986297748185	0.0
14	1.5737052733290005E-4	0.0	0.0	4.155447459488892	0.0
15	1.5737052733290005E-4	0.0	0.0	4.258446469628275	0.0
16	1.5737052733290005E-4	0.0	0.0	4.437455444469449	0.0
17	1.5737052733290005E-4	3.934263183322501E-5	0.0	4.66461980067449	0.0
18	1.5737052733290005E-4	3.934263183322501E-5	0.0	4.938051091915404	0.0
19	1.5737052733290005E-4	3.934263183322501E-5	0.0	5.089441539209654	0.0
20	1.5737052733290005E-4	3.934263183322501E-5	0.0	5.256096927655195	0.0
21	1.5737052733290005E-4	3.934263183322501E-5	0.0	5.45623289579081	0.0
22	1.5737052733290005E-4	3.934263183322501E-5	0.0	5.6857971525376785	0.0
23	1.5737052733290005E-4	3.934263183322501E-5	0.0	5.925944577247684	0.0
24	1.5737052733290005E-4	3.934263183322501E-5	0.0	6.102120882596865	0.0
25	1.5737052733290005E-4	3.934263183322501E-5	0.0	6.250521289871791	3.934263183322501E-5
26	1.5737052733290005E-4	3.934263183322501E-5	0.0	6.40545257403103	3.934263183322501E-5
27	1.5737052733290005E-4	3.934263183322501E-5	0.0	6.602205075828989	3.934263183322501E-5
28	1.9671315916612505E-4	3.934263183322501E-5	0.0	6.778696122232836	3.934263183322501E-5
29	1.9671315916612505E-4	3.934263183322501E-5	0.0	6.992208585191748	3.934263183322501E-5
30	1.9671315916612505E-4	3.934263183322501E-5	0.0	7.250650333704203	3.934263183322501E-5
31	1.9671315916612505E-4	3.934263183322501E-5	0.0	7.457907318201633	3.934263183322501E-5
32	1.9671315916612505E-4	3.934263183322501E-5	0.0	7.652220576825931	3.934263183322501E-5
33	1.9671315916612505E-4	3.934263183322501E-5	0.0	7.842874970689739	3.934263183322501E-5
34	1.9671315916612505E-4	3.934263183322501E-5	0.0	8.066183748975124	3.934263183322501E-5
35	1.9671315916612505E-4	3.934263183322501E-5	0.0	8.311367030559783	3.934263183322501E-5
36	1.9671315916612505E-4	3.934263183322501E-5	0.0	8.523935270354697	3.934263183322501E-5
37	1.9671315916612505E-4	3.934263183322501E-5	0.0	8.751020941296073	3.934263183322501E-5
38	2.3605579099935005E-4	3.934263183322501E-5	0.0	9.023075240422823	3.934263183322501E-5
39	2.3605579099935005E-4	3.934263183322501E-5	0.0	9.527054354206436	3.934263183322501E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGAT	20	7.0349016E-4	45.0	23
CGTTTTT	15315	0.0	43.48678	1
TCGATAG	110	0.0	42.954548	1
CGGTCTA	360	0.0	40.625	31
TTATACG	95	0.0	40.263157	1
TACGTAG	210	0.0	39.64286	1
CGGTTTT	1505	0.0	39.46844	1
AGGGCGA	1720	0.0	39.375	6
CGAGGGA	1730	0.0	39.27746	4
TGCGTAG	215	0.0	38.720932	1
GTCGCGC	35	6.251279E-6	38.571426	39
TAACGGG	715	0.0	38.391613	3
TACGGGA	1020	0.0	38.382355	4
CGTTAGG	460	0.0	38.152176	2
AAGGGAT	4240	0.0	38.04835	5
CGACGGT	385	0.0	37.987015	28
CTAACGG	190	0.0	37.894737	2
GGGCGAT	3530	0.0	37.861187	7
ACGGGAT	1090	0.0	37.56881	5
ACGGCGC	30	1.1403108E-4	37.500004	26
>>END_MODULE