FastQCFastQC Report
Thu 26 May 2016
SRR1547897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547897_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2803393
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180360.64336323876103No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35850.1278807502194662No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86100.041.7334481
TACGTAG1300.039.8076931
ACGGGTA2500.039.65
TAGGGAT40200.038.6753735
CGGCATA356.2515082E-638.57142632
TAAGGGA34400.038.3284874
CGGTCTA3350.038.28358531
TAGGGTA15800.038.1645555
GGGCGAT36850.037.9782877
CTCACGA3500.037.9285724
TAGGGCG8550.037.8947375
ACGGGAT15000.037.85
GTAGGGA35300.037.5424924
CACGACC2400.037.49999627
TTACGAG1200.037.4999961
AGGGATT47800.037.421556
AGGGCGA19400.037.229386
TGCGTAG1700.037.0588231
GCGCGAC2800.036.9642839
TAGCGGG17500.036.93
Produced by FastQC (version 0.11.5)