FastQCFastQC Report
Thu 26 May 2016
SRR1547896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547896_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1644350
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59370.36105452002310945No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38760.23571624045975614No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18810.1143917049289993No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT17930.10904004621887069Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC17810.10831027457658042No Hit
GAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT17550.10672910268495149No Hit
AAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT17060.10374920181226625No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC16470.1001611579043391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG302.1660744E-645.0000041
GTCGTAC302.1660744E-645.00000415
CCAACGA302.1660744E-645.0000042
GTTGACG207.0341746E-445.01
CTTAACG351.2123564E-745.01
AACGTCA207.0341746E-445.026
GTCGAAT207.0341746E-445.027
GGTCGAA207.0341746E-445.09
TAGCGGA253.891542E-545.03
CGACCGT207.0341746E-445.08
TGACGTA850.045.022
CCCGTCG207.0341746E-445.018
TGCGCAA207.0341746E-445.032
ACGGCCC207.0341746E-445.011
TACGAGT253.891542E-545.022
CGTAGAT253.891542E-545.034
GCGATCG207.0341746E-445.09
CCGACAG351.2123564E-745.030
ACCGTTG253.891542E-545.01
CGCAACA207.0341746E-445.034