##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547896_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1644350
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49104022866178	31.0	31.0	33.0	30.0	34.0
2	31.899506187855383	31.0	31.0	34.0	30.0	34.0
3	31.97865782832122	33.0	31.0	34.0	30.0	34.0
4	35.74027183993675	37.0	35.0	37.0	35.0	37.0
5	35.656221607322045	37.0	35.0	37.0	33.0	37.0
6	35.694299267187645	37.0	35.0	37.0	33.0	37.0
7	35.999010551281664	37.0	35.0	37.0	35.0	37.0
8	36.02370237479855	37.0	35.0	37.0	35.0	37.0
9	37.79801988627725	39.0	38.0	39.0	35.0	39.0
10	37.32596345060358	39.0	37.0	39.0	34.0	39.0
11	37.06664213823091	39.0	37.0	39.0	33.0	39.0
12	36.243640344208956	38.0	35.0	39.0	32.0	39.0
13	35.93049958950345	38.0	35.0	39.0	32.0	39.0
14	36.94086721196825	39.0	35.0	41.0	32.0	41.0
15	37.1926505914191	39.0	35.0	41.0	32.0	41.0
16	37.321741721652934	39.0	35.0	41.0	33.0	41.0
17	37.24245142457506	39.0	35.0	41.0	33.0	41.0
18	37.170227141423666	38.0	35.0	41.0	32.0	41.0
19	37.08416334721927	38.0	35.0	41.0	32.0	41.0
20	36.944136588925716	38.0	35.0	41.0	32.0	41.0
21	36.814034724967314	38.0	35.0	41.0	32.0	41.0
22	36.70919451454982	38.0	35.0	41.0	32.0	41.0
23	36.65708395414602	38.0	35.0	41.0	32.0	41.0
24	36.67082737859945	38.0	35.0	41.0	32.0	41.0
25	36.6335220603886	38.0	35.0	41.0	32.0	41.0
26	36.518876151670874	38.0	35.0	40.0	31.0	41.0
27	36.45496396752516	38.0	35.0	40.0	31.0	41.0
28	36.437175175601304	38.0	35.0	40.0	31.0	41.0
29	36.47822726305227	38.0	35.0	40.0	31.0	41.0
30	36.42519171709186	38.0	35.0	40.0	31.0	41.0
31	36.25998053942287	38.0	35.0	40.0	31.0	41.0
32	36.071270714872135	38.0	35.0	40.0	30.0	41.0
33	35.97019430169976	38.0	35.0	40.0	30.0	41.0
34	35.8757101590294	38.0	35.0	40.0	30.0	41.0
35	35.75826314349135	38.0	35.0	40.0	30.0	41.0
36	35.62018791619789	38.0	35.0	40.0	29.0	41.0
37	35.487219874114395	37.0	34.0	40.0	29.0	41.0
38	35.424873658284426	37.0	34.0	40.0	29.0	41.0
39	35.336688661173106	37.0	34.0	40.0	28.0	41.0
40	35.164564721622526	37.0	34.0	40.0	27.0	41.0
41	35.117314440356374	37.0	34.0	40.0	27.0	41.0
42	35.01989965639918	37.0	34.0	40.0	27.0	41.0
43	34.94302490345729	37.0	34.0	40.0	27.0	41.0
44	34.78834007358531	36.0	34.0	40.0	26.0	41.0
45	34.65095995378113	36.0	34.0	40.0	26.0	41.0
46	34.587211968254934	36.0	34.0	40.0	25.0	41.0
47	34.49207954510901	36.0	34.0	40.0	25.0	41.0
48	34.4196290327485	36.0	34.0	40.0	24.0	41.0
49	34.287568340073584	36.0	34.0	40.0	24.0	41.0
50	34.17683704807371	35.0	34.0	40.0	24.0	41.0
51	33.81402012953447	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	5.0
12	5.0
13	10.0
14	24.0
15	42.0
16	95.0
17	239.0
18	555.0
19	1155.0
20	2017.0
21	3182.0
22	5002.0
23	7774.0
24	11653.0
25	16462.0
26	22107.0
27	25518.0
28	28741.0
29	33252.0
30	39791.0
31	47952.0
32	60484.0
33	80391.0
34	148508.0
35	246247.0
36	108821.0
37	137851.0
38	218590.0
39	397801.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.65387539149208	24.383190926505915	29.963146532064343	12.999787149937667
2	29.150667436981177	26.780247514215343	30.56447836531152	13.504606683491957
3	28.90807918022319	25.569313102441694	31.98941831118679	13.533189406148328
4	25.068142427098856	28.36111533432663	31.087177304101925	15.483564934472588
5	23.033295831179494	32.57426946817892	29.61808617386809	14.774348526773498
6	21.452610453978778	39.820962690424786	27.33706327728282	11.389363578313619
7	87.78988658132393	3.94526712682823	6.43482227019795	1.8300240216498922
8	88.02730562228236	3.0911302338309974	5.64314166692006	3.2384224769665826
9	83.57880013379146	5.068507312919999	8.030528780369142	3.3221637729193905
10	46.94547997689057	26.79386991820476	13.548757867850519	12.711892237054156
11	40.51746890868732	20.92845197190379	22.858393894243925	15.69568522516496
12	37.635174993158394	20.83698725940341	24.840210417490194	16.687627329948004
13	21.44847508133913	37.67324474716453	25.154377109496153	15.723903062000183
14	15.420561316021528	39.313528141818956	26.960744367075133	18.30516617508438
15	14.046705385106579	26.164016176604736	44.01441298993523	15.774865448353454
16	16.194301699759784	21.209718125703166	42.26296104843859	20.33301912609846
17	16.646881746586796	22.719676467905252	26.608872806884182	34.02456897862377
18	20.44692431659926	24.743394046279686	32.777754127770855	22.0319275093502
19	24.80706662206951	27.785751208684285	26.057165445312734	21.350016723933468
20	28.161766047374343	24.771246997293765	25.640891537689665	21.42609541764223
21	21.345577279776204	29.266092985070085	28.09973545777967	21.288594277374038
22	21.52802019034877	24.52932769787454	26.610697235989907	27.331954875786785
23	19.23562501900447	29.482348648402105	25.54285888040867	25.739167452184752
24	20.375406695654817	25.201143308906254	35.37932921823213	19.0441207772068
25	17.48763949280871	27.240550977589926	32.24112871347341	23.03068081612795
26	17.692279624167604	32.77726761334266	26.773862012345305	22.756590750144433
27	17.605436798735063	32.41165202055524	28.66257183689604	21.320339343813664
28	14.327120138656612	28.413476449660962	37.26189679812692	19.997506613555508
29	15.213366983914616	23.725058533767143	37.3986073524493	23.662967129868946
30	18.104965487882748	28.952412807492323	32.20020068720163	20.7424210174233
31	26.250737373430233	26.8266488278043	24.991394776051326	21.931219022714142
32	25.769696232553898	26.85973180892146	27.73685650865084	19.633715449873808
33	23.965518289901784	27.311582084106185	26.75233374889774	21.97056587709429
34	18.980326572809926	26.453370632772828	29.6193632742421	24.946939520175146
35	19.292547207103112	24.892328275610424	31.322832730258156	24.492291787028307
36	27.074406300361847	23.653966612947368	29.023930428436767	20.24769665825402
37	20.107823760148385	28.32480919512269	30.965791954267647	20.601575090461278
38	19.99172925472071	29.410040441511843	27.24176726366041	23.356463040107034
39	19.353726396448444	28.524766625110225	30.528962812053393	21.592544166387935
40	23.343570407759906	25.53215556298841	27.624228418524037	23.500045610727643
41	18.075531364977042	23.39605315170128	30.224952108735977	28.303463374585704
42	22.046948642320675	25.007206494967615	26.917627025876484	26.02821783683522
43	21.61279532946149	25.34478669383039	27.787514823486486	25.254903153221637
44	19.227415088028703	28.688965244625535	27.9736065922705	24.11001307507526
45	17.988506096633927	33.0214978562958	24.73214340012771	24.25785264694256
46	23.403107610910087	28.88715905981087	27.262444126858636	20.44728920242041
47	21.452306382461156	26.073524492960743	28.787362787727673	23.686806336850427
48	21.784352479703227	22.832547815246144	31.129260802140664	24.253838902909962
49	20.442363243834947	22.325721409675555	32.226533280627606	25.00538206586189
50	19.020099127314744	27.556116398576947	30.64603034633746	22.77775412777085
51	17.871560190956913	28.918904126250496	27.132848846048592	26.076686836744003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1167.0
1	1523.5
2	1880.0
3	5825.5
4	9771.0
5	7551.5
6	5332.0
7	5484.0
8	5636.0
9	6040.0
10	6444.0
11	6623.5
12	6803.0
13	6653.0
14	6503.0
15	6315.0
16	6127.0
17	5942.5
18	5758.0
19	5655.0
20	5552.0
21	5802.0
22	6052.0
23	6118.0
24	6184.0
25	6877.0
26	9918.5
27	12267.0
28	14863.5
29	17460.0
30	19928.0
31	22396.0
32	28611.0
33	34826.0
34	39601.5
35	44377.0
36	46640.5
37	48904.0
38	55057.5
39	61211.0
40	80681.0
41	100151.0
42	125500.0
43	150849.0
44	156909.0
45	162969.0
46	167179.0
47	171389.0
48	168564.0
49	165739.0
50	153690.5
51	141642.0
52	125724.5
53	109807.0
54	94548.0
55	79289.0
56	70715.5
57	62142.0
58	56886.5
59	51631.0
60	45781.5
61	39932.0
62	34980.0
63	30028.0
64	26247.5
65	22467.0
66	18456.5
67	14446.0
68	11209.0
69	7972.0
70	6539.0
71	5106.0
72	4334.5
73	3563.0
74	2606.5
75	1225.5
76	801.0
77	527.5
78	254.0
79	220.0
80	186.0
81	117.5
82	49.0
83	39.0
84	29.0
85	20.5
86	12.0
87	10.5
88	9.0
89	5.5
90	2.0
91	3.5
92	5.0
93	5.5
94	6.0
95	5.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1644350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.929715275692548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46230888443671	15.43801760664573
2	9.27433188130062	3.696695875331988
3	3.314377372988344	1.981637920795666
4	1.6367734854812626	1.304817181457778
5	0.9232983956490223	0.9200537069894368
6	0.5788352459708273	0.692161298624043
7	0.41137279849834596	0.5738979922365814
8	0.2984842097568813	0.4758964251795788
9	0.23373393979107723	0.4192425783271831
>10	2.6586270982874103	13.39140811804336
>50	2.1817374782476464	32.42572111151851
>100	1.015944349641043	26.277236956193622
>500	0.007091569056654994	1.023619268274811
>1k	0.002774961804778041	1.0147705964875875
>5k	3.083290894197823E-4	0.36482336389407816
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5937	0.36105452002310945	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3876	0.23571624045975614	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1881	0.1143917049289993	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT	1793	0.10904004621887069	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	1781	0.10831027457658042	No Hit
GAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	1755	0.10672910268495149	No Hit
AAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	1706	0.10374920181226625	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	1647	0.1001611579043391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1454678140298598	0.0
2	0.0	0.0	0.0	0.5559035485146107	0.0
3	0.0	0.0	0.0	0.8027488065192934	0.0
4	0.0	0.0	0.0	1.3933773223462158	0.0
5	0.0	0.0	0.0	2.240885456259312	0.0
6	0.0	0.0	0.0	3.129747316568857	0.0
7	0.0	0.0	0.0	3.581111077325387	0.0
8	0.0	0.0	0.0	4.647125003800894	0.0
9	0.0	0.0	0.0	4.975218171313893	0.0
10	0.0	0.0	0.0	5.683279107246024	0.0
11	0.0	0.0	0.0	6.5691610666828835	0.0
12	0.0	0.0	0.0	7.267917414175814	0.0
13	0.0	0.0	0.0	7.52090491683644	0.0
14	0.0	0.0	0.0	7.61875513120686	0.0
15	0.0	0.0	0.0	7.780156292760057	0.0
16	0.0	0.0	0.0	8.149603186669504	0.0
17	0.0	0.0	0.0	8.567640709094778	0.0
18	0.0	0.0	0.0	9.14750509319792	0.0
19	0.0	0.0	0.0	9.408033569495545	0.0
20	0.0	0.0	0.0	9.679751877641621	0.0
21	0.0	0.0	0.0	10.047435156748866	0.0
22	0.0	0.0	0.0	10.400887888831454	0.0
23	0.0	0.0	0.0	10.804938121446163	0.0
24	0.0	0.0	0.0	11.09496153495302	0.0
25	0.0	0.0	0.0	11.33943503512026	0.0
26	0.0	0.0	0.0	11.55502174111351	0.0
27	0.0	0.0	0.0	11.76203363030985	0.0
28	0.0	0.0	0.0	11.991060297381944	0.0
29	0.0	0.0	0.0	12.236324383494997	0.0
30	0.0	0.0	0.0	12.56301882202694	0.0
31	6.081430352418889E-5	0.0	0.0	12.839784717365525	0.0
32	6.081430352418889E-5	0.0	0.0	13.082372974123514	0.0
33	6.081430352418889E-5	0.0	0.0	13.337306534496914	0.0
34	6.081430352418889E-5	0.0	0.0	13.591936023352693	0.0
35	6.081430352418889E-5	0.0	0.0	13.945449569738802	0.0
36	6.081430352418889E-5	0.0	0.0	14.204336059841275	0.0
37	6.081430352418889E-5	0.0	0.0	14.462857664122602	0.0
38	6.081430352418889E-5	0.0	0.0	14.711709794143582	0.0
39	6.081430352418889E-5	0.0	0.0	14.971569313102442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	30	2.1660744E-6	45.000004	1
GTCGTAC	30	2.1660744E-6	45.000004	15
CCAACGA	30	2.1660744E-6	45.000004	2
GTTGACG	20	7.0341746E-4	45.0	1
CTTAACG	35	1.2123564E-7	45.0	1
AACGTCA	20	7.0341746E-4	45.0	26
GTCGAAT	20	7.0341746E-4	45.0	27
GGTCGAA	20	7.0341746E-4	45.0	9
TAGCGGA	25	3.891542E-5	45.0	3
CGACCGT	20	7.0341746E-4	45.0	8
TGACGTA	85	0.0	45.0	22
CCCGTCG	20	7.0341746E-4	45.0	18
TGCGCAA	20	7.0341746E-4	45.0	32
ACGGCCC	20	7.0341746E-4	45.0	11
TACGAGT	25	3.891542E-5	45.0	22
CGTAGAT	25	3.891542E-5	45.0	34
GCGATCG	20	7.0341746E-4	45.0	9
CCGACAG	35	1.2123564E-7	45.0	30
ACCGTTG	25	3.891542E-5	45.0	1
CGCAACA	20	7.0341746E-4	45.0	34
>>END_MODULE