FastQCFastQC Report
Thu 26 May 2016
SRR1547895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547895_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2716093
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117270.43175988451058195No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30450.11210956325869549No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG207.034986E-445.00000423
TACGTAG950.040.2631571
CGTTTTT62050.040.2497981
TATCACG850.039.7058831
GCGATAA1750.038.5714269
TGTTACG1000.038.251
CGCATCG659.094947E-1238.0769221
TCGATAG1900.037.8947371
CTATCCG601.5643309E-1037.4999961
TCGTAAG1200.037.4999961
ATTGCGA301.14033195E-437.49999611
GGGCGAT38650.037.4902957
TACGGGA10900.037.1559644
TCACGAC3100.037.01613225
AGGGATT47950.036.9760176
TAGGGAG34150.036.9619335
GCGAGAC3350.036.94029621
GTAGGGA40550.036.898894
GACCGAT8850.036.8644079
ACCGTTC5250.036.85714345
Produced by FastQC (version 0.11.5)