FastQCFastQC Report
Thu 26 May 2016
SRR1547894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547894_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3450191
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140760.4079774134243583No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45940.13315204868368158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA1150.041.08695624
ACCGTTC14750.040.27118745
CGTTTTT75750.040.00991
CGGTCTA6200.039.91935331
CGTAAGG4850.039.4329872
CGTAACG403.4609002E-739.37537
TACGGGA13100.038.8167954
TAAGGGA34850.038.6083224
TACGCGG1300.038.0769272
AGGGCGA27000.037.9166686
ACGTAAG1900.037.8947371
CACGACG6600.037.84090826
ACGGTCT6550.037.7862630
GCGATAA3400.037.720599
ATAGCGG5800.037.6293142
TAGGGAC36450.037.530865
CCTTACG301.1403956E-437.50000445
CAACGTA301.1403956E-437.50000425
CGACTAT301.1403956E-437.50000412
GGGCGAT56250.037.447
Produced by FastQC (version 0.11.5)