##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547894_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3450191
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.487560833588635	31.0	31.0	33.0	30.0	34.0
2	31.903962128473466	31.0	31.0	34.0	30.0	34.0
3	31.995331562803333	33.0	31.0	34.0	30.0	34.0
4	35.76177202943257	37.0	35.0	37.0	35.0	37.0
5	35.64179171529924	37.0	35.0	37.0	33.0	37.0
6	35.689418353940404	37.0	35.0	37.0	33.0	37.0
7	36.001203121798184	37.0	35.0	37.0	35.0	37.0
8	36.020709578107414	37.0	35.0	37.0	35.0	37.0
9	37.76839977844705	39.0	38.0	39.0	35.0	39.0
10	37.31429216527433	39.0	37.0	39.0	34.0	39.0
11	37.02242455562605	39.0	37.0	39.0	33.0	39.0
12	36.27439669282077	38.0	35.0	39.0	32.0	39.0
13	36.00103588468001	38.0	35.0	39.0	32.0	39.0
14	36.92894103543833	39.0	35.0	41.0	31.0	41.0
15	37.17114646696371	39.0	35.0	41.0	32.0	41.0
16	37.29177254244765	39.0	35.0	41.0	33.0	41.0
17	37.21996289480785	39.0	35.0	41.0	32.0	41.0
18	37.152552713748314	39.0	35.0	41.0	32.0	41.0
19	37.09653175722735	39.0	35.0	41.0	32.0	41.0
20	36.92835179269785	38.0	35.0	41.0	32.0	41.0
21	36.79784916255361	38.0	35.0	41.0	31.0	41.0
22	36.72686787485099	38.0	35.0	41.0	31.0	41.0
23	36.659889843779666	38.0	35.0	41.0	31.0	41.0
24	36.67792826542067	38.0	35.0	41.0	31.0	41.0
25	36.629418777105386	38.0	35.0	41.0	31.0	41.0
26	36.52269830858639	38.0	35.0	41.0	31.0	41.0
27	36.43438493694987	38.0	35.0	40.0	31.0	41.0
28	36.39847301207382	38.0	35.0	40.0	31.0	41.0
29	36.39851706760582	38.0	35.0	40.0	31.0	41.0
30	36.314668956008525	38.0	35.0	40.0	31.0	41.0
31	36.1353571439958	38.0	35.0	40.0	30.0	41.0
32	36.02784628445208	38.0	35.0	40.0	30.0	41.0
33	35.92171476883453	38.0	35.0	40.0	30.0	41.0
34	35.783582416161885	38.0	35.0	40.0	30.0	41.0
35	35.719812033594664	38.0	35.0	40.0	29.0	41.0
36	35.60490853984606	38.0	35.0	40.0	29.0	41.0
37	35.491614812049534	38.0	34.0	40.0	29.0	41.0
38	35.37686870089221	37.0	34.0	40.0	28.0	41.0
39	35.31069178488959	37.0	34.0	40.0	27.0	41.0
40	35.11052315654409	37.0	34.0	40.0	27.0	41.0
41	35.05003433143266	37.0	34.0	40.0	26.0	41.0
42	34.978463800989566	37.0	34.0	40.0	26.0	41.0
43	34.90227120759401	37.0	34.0	40.0	26.0	41.0
44	34.6961730524484	36.0	34.0	40.0	26.0	41.0
45	34.5467146601449	36.0	34.0	40.0	25.0	41.0
46	34.54551733512724	36.0	34.0	40.0	25.0	41.0
47	34.45660225767211	36.0	34.0	40.0	24.0	41.0
48	34.36676810066457	36.0	34.0	40.0	24.0	41.0
49	34.23635561045751	36.0	33.0	40.0	24.0	41.0
50	34.13580842335975	35.0	33.0	40.0	24.0	41.0
51	33.821375396318636	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	5.0
11	7.0
12	8.0
13	18.0
14	38.0
15	126.0
16	298.0
17	705.0
18	1556.0
19	3008.0
20	5269.0
21	8290.0
22	12454.0
23	18086.0
24	26124.0
25	35921.0
26	46804.0
27	55592.0
28	62243.0
29	71116.0
30	83783.0
31	102079.0
32	126688.0
33	168735.0
34	307117.0
35	460851.0
36	236793.0
37	305692.0
38	479430.0
39	831226.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.426876656973484	23.522089066953107	29.27936453373161	12.771669742341802
2	30.460313646403925	25.914536325670085	29.774525526267965	13.850624501658023
3	29.76139581837643	25.867002725356365	30.525527427322142	13.846074028945063
4	26.58887580426707	27.723247785412457	29.590477744565447	16.097398665755026
5	23.547768804683567	32.5926303790138	28.796289828592098	15.063310987710535
6	22.851169688866502	40.17392080612348	25.965432058688926	11.009477446321087
7	88.55877834009769	3.882683596357419	5.766173524886013	1.7923645386588742
8	89.38603688897223	3.0705256607532743	5.355732479738078	2.187704970536414
9	84.67203699737202	5.394599893165335	6.89376327281591	3.0395998366467247
10	48.05104992738084	30.21145785842001	11.748682899004722	9.988809315194434
11	40.90231526312601	20.88791606029927	22.159092061859763	16.050676614714952
12	37.13892940999498	20.221576138828258	25.839497001760193	16.79999744941657
13	24.730572887124218	31.731518631867043	26.32503533862328	17.21287314238545
14	18.502772745045128	34.8381582352977	29.349418626389088	17.309650393268083
15	17.89898008545034	24.578639269536094	40.725397521470555	16.796983123543015
16	22.69796077956264	21.30316843328384	37.98601294826866	18.01285783888486
17	22.00199930960344	22.026316803910277	26.393292429317682	29.5783914571686
18	24.840363910287866	24.496730760702814	30.519643695088185	20.143261633921135
19	27.9849434422616	26.315644554171058	25.152491557713763	20.54692044585358
20	30.359739504276718	25.58620667667384	24.524990065767373	19.529063753282063
21	25.632928727713917	25.94450568098984	28.51740671748318	19.905158873813072
22	24.440357070086844	22.833025765819922	28.123138690002957	24.603478474090277
23	21.501389343372583	27.74492774457994	26.908510282474218	23.84517262957326
24	22.898239546738136	25.312308796817334	32.61610734014436	19.17334431630017
25	21.773055462726557	24.82091571162292	30.884782900424934	22.52124592522559
26	20.18302754832993	29.902025713938734	26.264690853346963	23.65025588438437
27	19.57700312823261	29.96167458555193	29.340694471697365	21.120627814518095
28	17.541898405044822	27.669627565546374	33.72590097185924	21.062573057549567
29	19.159490010842877	25.74967588750884	33.11845634053303	21.972377761115254
30	21.7607662880113	26.846600666455856	31.230097116362543	20.162535929170296
31	27.813938416742722	26.396509642509645	25.27280373753221	20.516748203215414
32	28.364255775984574	26.386133405367996	25.50148672928542	19.74812408936201
33	26.301529393590094	26.91343754592137	25.647333727321183	21.137699333167355
34	20.539790405806517	27.52972226754983	28.788493158784544	23.14199416785911
35	21.096107432892847	28.01566637904974	28.722815635424244	22.165410552633173
36	27.88364470256864	25.906188961712555	26.804863846668198	19.405302489050605
37	22.245753930724415	29.56810217173484	27.37877410265113	20.807369794889617
38	21.735724196138705	30.91144809084482	24.240657980963952	23.112169732052514
39	22.093269618986312	28.51581260283851	26.652785309566916	22.73813246860826
40	24.873898285631142	25.75637116901644	25.82729477875283	23.54243576659959
41	20.417536304511838	24.871637541225976	27.251737657422442	27.45908849683974
42	23.517364690824362	26.0624411807926	25.3921014807586	25.028092647624433
43	22.501102112897517	25.524412996266005	26.445608373565406	25.528876517271073
44	21.19676852672794	28.368487425768603	27.255824387693316	23.178919659810138
45	20.362669776832647	31.595758031946637	24.14382276227606	23.89774942894466
46	23.238974306060157	28.68052812148661	26.236721387308705	21.843776185144534
47	21.483970017891764	27.608065756359572	26.929726499199607	23.97823772654905
48	24.055972553403564	24.891288627209335	28.525609161927555	22.527129657459543
49	23.06805623224917	23.019160388511825	29.68206687687725	24.230716502361755
50	20.569556873807855	28.025955664483504	29.007176704130295	22.39731075757835
51	20.040832522025593	29.365417740641025	26.329730730849395	24.264019006483988
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1809.0
1	3242.5
2	4676.0
3	14614.0
4	24552.0
5	18089.0
6	11626.0
7	11065.0
8	10504.0
9	11318.5
10	12133.0
11	12231.5
12	12330.0
13	12043.0
14	11756.0
15	11630.5
16	11505.0
17	10959.5
18	10414.0
19	10580.0
20	10746.0
21	12025.0
22	13304.0
23	14196.5
24	15089.0
25	17228.5
26	22523.5
27	25679.0
28	30618.0
29	35557.0
30	43271.0
31	50985.0
32	57205.5
33	63426.0
34	73860.0
35	84294.0
36	89379.0
37	94464.0
38	108334.0
39	122204.0
40	159518.5
41	196833.0
42	228341.5
43	259850.0
44	268812.5
45	277775.0
46	280671.5
47	283568.0
48	284176.0
49	284784.0
50	270697.5
51	256611.0
52	242282.5
53	227954.0
54	213482.0
55	199010.0
56	196231.0
57	193452.0
58	183290.5
59	173129.0
60	155934.0
61	138739.0
62	120823.5
63	102908.0
64	89307.5
65	75707.0
66	62639.0
67	49571.0
68	41049.5
69	32528.0
70	27243.0
71	21958.0
72	17334.0
73	12710.0
74	10043.5
75	5637.5
76	3898.0
77	3195.5
78	2493.0
79	2047.0
80	1601.0
81	1170.0
82	739.0
83	498.5
84	258.0
85	204.0
86	150.0
87	117.5
88	85.0
89	68.5
90	52.0
91	34.0
92	16.0
93	12.0
94	8.0
95	4.5
96	1.0
97	2.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3450191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.04951139540714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94819728283844	13.28978677825198
2	8.92326341202912	3.042745624552202
3	3.180462981092319	1.626760195164122
4	1.5049070051485176	1.0263171653323073
5	0.9020862844139259	0.7690065192877859
6	0.588348517290451	0.6018632850008664
7	0.40851114432755725	0.4875440787254449
8	0.3269531352340153	0.4459512963949148
9	0.24635291646819085	0.3780177170954583
>10	2.6204927146826136	10.548999285400855
>50	1.5004338914877537	19.146323466470815
>100	1.8394391572647835	46.46801805094545
>500	0.006919054244075027	0.7824422642710507
>1k	0.0034595271220375133	0.9711001800880916
>5k	0.0	0.0
>10k+	1.729763561018757E-4	0.4151240930187068
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14076	0.4079774134243583	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4594	0.13315204868368158	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.695170789095445E-5	0.0	0.0	0.04628729250061808	0.0
2	8.695170789095445E-5	0.0	0.0	0.17561346603709765	0.0
3	8.695170789095445E-5	0.0	0.0	0.26250720612279144	0.0
4	8.695170789095445E-5	0.0	0.0	0.46006148645103995	0.0
5	8.695170789095445E-5	0.0	0.0	0.8131144043909453	0.0
6	8.695170789095445E-5	0.0	0.0	1.1937889815375438	0.0
7	8.695170789095445E-5	0.0	0.0	1.4000674165575182	0.0
8	8.695170789095445E-5	0.0	0.0	1.8895185802757006	0.0
9	8.695170789095445E-5	0.0	0.0	2.0677695814521573	0.0
10	8.695170789095445E-5	0.0	0.0	2.413141765195028	0.0
11	8.695170789095445E-5	0.0	0.0	2.853697085175864	0.0
12	8.695170789095445E-5	0.0	0.0	3.206141341160533	0.0
13	8.695170789095445E-5	0.0	0.0	3.345177122078169	0.0
14	8.695170789095445E-5	0.0	0.0	3.405840430283425	0.0
15	8.695170789095445E-5	0.0	0.0	3.5093129626736608	0.0
16	8.695170789095445E-5	0.0	0.0	3.7294167192482965	0.0
17	8.695170789095445E-5	0.0	0.0	4.019719487993563	0.0
18	8.695170789095445E-5	0.0	0.0	4.375351973267567	0.0
19	8.695170789095445E-5	0.0	0.0	4.557689704714899	0.0
20	8.695170789095445E-5	0.0	0.0	4.76104076556921	0.0
21	8.695170789095445E-5	0.0	0.0	4.9931438578327985	0.0
22	8.695170789095445E-5	0.0	0.0	5.264549122063097	0.0
23	8.695170789095445E-5	0.0	0.0	5.543345281464128	0.0
24	8.695170789095445E-5	0.0	0.0	5.7413343203318306	0.0
25	8.695170789095445E-5	0.0	0.0	5.910165553153434	0.0
26	8.695170789095445E-5	0.0	0.0	6.075982460101484	0.0
27	8.695170789095445E-5	0.0	0.0	6.2695079779641185	0.0
28	8.695170789095445E-5	0.0	0.0	6.46184515581891	0.0
29	8.695170789095445E-5	0.0	0.0	6.677891166025301	0.0
30	8.695170789095445E-5	0.0	0.0	6.9545715005343185	0.0
31	8.695170789095445E-5	0.0	0.0	7.1908482747766715	0.0
32	8.695170789095445E-5	0.0	0.0	7.397706387849253	0.0
33	8.695170789095445E-5	0.0	0.0	7.614216140497729	0.0
34	8.695170789095445E-5	0.0	0.0	7.853536224516266	0.0
35	8.695170789095445E-5	0.0	0.0	8.13088318878578	0.0
36	8.695170789095445E-5	0.0	0.0	8.352001381952478	0.0
37	8.695170789095445E-5	0.0	0.0	8.586828961063315	0.0
38	8.695170789095445E-5	0.0	0.0	8.857422676019965	0.0
39	8.695170789095445E-5	0.0	0.0	9.298934464787601	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	115	0.0	41.086956	24
ACCGTTC	1475	0.0	40.271187	45
CGTTTTT	7575	0.0	40.0099	1
CGGTCTA	620	0.0	39.919353	31
CGTAAGG	485	0.0	39.432987	2
CGTAACG	40	3.4609002E-7	39.375	37
TACGGGA	1310	0.0	38.816795	4
TAAGGGA	3485	0.0	38.608322	4
TACGCGG	130	0.0	38.076927	2
AGGGCGA	2700	0.0	37.916668	6
ACGTAAG	190	0.0	37.894737	1
CACGACG	660	0.0	37.840908	26
ACGGTCT	655	0.0	37.78626	30
GCGATAA	340	0.0	37.72059	9
ATAGCGG	580	0.0	37.629314	2
TAGGGAC	3645	0.0	37.53086	5
CCTTACG	30	1.1403956E-4	37.500004	45
CAACGTA	30	1.1403956E-4	37.500004	25
CGACTAT	30	1.1403956E-4	37.500004	12
GGGCGAT	5625	0.0	37.44	7
>>END_MODULE