FastQCFastQC Report
Thu 26 May 2016
SRR1547893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547893_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2291411
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94720.41336975339648807No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35830.15636653572842235No Hit
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC24480.10683373694199774Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG351.2126293E-745.00000444
CGGCCTA207.0347544E-445.00000444
TCCGAAC600.045.00000436
TATCGAT207.0347544E-445.0000041
AGTAACG551.8189894E-1245.01
CGACGGT4950.042.27272828
CGGGTAT1950.041.5384646
CGTTTTT47850.041.520381
TCACGAC5200.040.6730825
ATTAACG1550.040.645161
CGTTAGG3550.040.563382
TGCGTAG1800.040.01
GTAGGGC9000.039.54
TTAACGG2850.039.4736862
ACGGTCT5500.038.86363630
CACGACG5400.038.7526
GGTACGA356.251006E-638.5714336
CGTAAGG4200.038.571432
TAGGGAC21300.038.556345
CTCACGA5500.038.45454424
Produced by FastQC (version 0.11.5)