##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547893_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2291411
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.428720993309362	31.0	31.0	33.0	30.0	34.0
2	31.838149943419143	31.0	31.0	34.0	30.0	34.0
3	31.927299380163575	33.0	31.0	34.0	30.0	34.0
4	35.693939672978786	37.0	35.0	37.0	33.0	37.0
5	35.55864705196929	37.0	35.0	37.0	33.0	37.0
6	35.609015143944056	37.0	35.0	37.0	33.0	37.0
7	35.95906321476156	37.0	35.0	37.0	35.0	37.0
8	35.984966468259074	37.0	35.0	37.0	35.0	37.0
9	37.72776424657122	39.0	38.0	39.0	35.0	39.0
10	37.22820000427684	39.0	37.0	39.0	34.0	39.0
11	36.961363980534266	39.0	37.0	39.0	33.0	39.0
12	36.24229481310861	38.0	35.0	39.0	32.0	39.0
13	35.93847589978402	38.0	35.0	39.0	32.0	39.0
14	36.81043819725051	39.0	35.0	41.0	31.0	41.0
15	37.080563460679905	39.0	35.0	41.0	32.0	41.0
16	37.25265916939388	39.0	35.0	41.0	32.0	41.0
17	37.20418510690575	39.0	35.0	41.0	32.0	41.0
18	37.12867922864994	39.0	35.0	41.0	32.0	41.0
19	37.05836142010316	38.0	35.0	41.0	32.0	41.0
20	36.90426902899567	38.0	35.0	41.0	32.0	41.0
21	36.775413489766784	38.0	35.0	41.0	31.0	41.0
22	36.71758536552369	38.0	35.0	41.0	31.0	41.0
23	36.66576358409731	38.0	35.0	41.0	31.0	41.0
24	36.68657041447388	38.0	35.0	41.0	31.0	41.0
25	36.6520968957555	38.0	35.0	41.0	31.0	41.0
26	36.5347770434898	38.0	35.0	40.0	31.0	41.0
27	36.45654358820831	38.0	35.0	40.0	31.0	41.0
28	36.400292221692226	38.0	35.0	40.0	31.0	41.0
29	36.424754878107855	38.0	35.0	40.0	31.0	41.0
30	36.35541856087799	38.0	35.0	40.0	31.0	41.0
31	36.17949289760763	38.0	35.0	40.0	30.0	41.0
32	36.08256659324757	38.0	35.0	40.0	30.0	41.0
33	35.99925984469831	38.0	35.0	40.0	30.0	41.0
34	35.88711409694725	38.0	35.0	40.0	30.0	41.0
35	35.852292321194234	38.0	35.0	40.0	30.0	41.0
36	35.72678188242965	38.0	35.0	40.0	29.0	41.0
37	35.608206471907486	38.0	34.0	40.0	29.0	41.0
38	35.48958611091594	38.0	34.0	40.0	29.0	41.0
39	35.41635699575502	38.0	34.0	40.0	28.0	41.0
40	35.227635286729445	37.0	34.0	40.0	27.0	41.0
41	35.186062648734776	37.0	34.0	40.0	27.0	41.0
42	35.12223036373658	37.0	34.0	40.0	27.0	41.0
43	35.0677874898916	37.0	34.0	40.0	27.0	41.0
44	34.86354128526048	37.0	34.0	40.0	26.0	41.0
45	34.727943175624105	37.0	34.0	40.0	26.0	41.0
46	34.73372694815553	36.0	34.0	40.0	26.0	41.0
47	34.63445885526429	36.0	34.0	40.0	25.0	41.0
48	34.56023384717975	36.0	34.0	40.0	25.0	41.0
49	34.450937871905126	36.0	34.0	40.0	24.0	41.0
50	34.33127099416037	36.0	34.0	40.0	24.0	41.0
51	33.99725933060459	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	5.0
12	1.0
13	9.0
14	21.0
15	64.0
16	181.0
17	438.0
18	989.0
19	1859.0
20	3077.0
21	5047.0
22	7774.0
23	11371.0
24	16501.0
25	23615.0
26	31129.0
27	36238.0
28	40986.0
29	47764.0
30	56374.0
31	69329.0
32	85717.0
33	113400.0
34	199878.0
35	301045.0
36	156885.0
37	199728.0
38	311269.0
39	570634.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.8816611249575	24.551291758658746	29.973845809416122	12.593201306967627
2	29.989076599527543	25.99782404815199	30.116596280632322	13.896503071688143
3	29.199301216586637	25.668812797005863	31.066796833915873	14.06508915249163
4	26.132326326442527	27.975208288691988	30.53520298191813	15.357262402947356
5	23.685580631322797	31.999540894235036	29.396079533527598	14.918798940914572
6	21.144788080357475	40.97754614951224	26.61635123511234	11.261314535017943
7	87.96440271954704	3.7269612478948564	6.219399313348849	2.089236719209256
8	88.48272963689186	2.832054136075981	5.734457938798408	2.9507582882337564
9	84.05113705048986	4.953847214663804	7.860353293232859	3.134662441613486
10	46.202187211285974	28.221388480722144	13.582548045723792	11.993876262268095
11	39.01177047679356	22.24349974753547	23.04881140921467	15.695918366456302
12	36.09474686121346	21.71208046046737	25.58772738718632	16.605445291132845
13	24.00647461324049	33.25632110520548	26.99847386610259	15.73873041545144
14	16.687665372995067	36.83468395674107	29.571211799192724	16.906438871071146
15	15.450829205236424	26.634244140400824	42.21634617272938	15.69858048163337
16	19.51465712611138	23.040650498753827	39.63365803864955	17.81103433648525
17	19.857546289164187	23.210720381459286	27.36785325722884	29.563880072147686
18	22.355657714831604	25.36816834692685	32.04968467027521	20.226489267966333
19	26.45828269131989	27.227764901189705	26.233224855776637	20.08072755171377
20	27.393776149280946	27.391026751639057	25.41743929831881	19.79775780076119
21	22.925874057513035	27.292790337482014	29.701306313009756	20.080029291995196
22	21.974320626024753	24.210584657226487	28.589982329665	25.225112387083765
23	19.45984373820323	29.016357170319946	27.677051388860402	23.846747702616423
24	20.288372535525053	26.80902727620667	33.804542266751795	19.09805792151648
25	20.063183776284568	27.041111350168084	30.69383886173192	22.201866011815426
26	18.034608370126527	32.64407825571231	26.840274398612905	22.481038975548255
27	18.629307444190502	31.887033797079617	29.392762799864364	20.09089595886552
28	15.978538987549593	28.806311918726056	34.53749676509364	20.6776523286307
29	17.110854403684016	26.6743940742189	33.89627613727961	22.318475384817475
30	20.46189007559098	28.912621960879125	30.21937138296011	20.406116580569787
31	25.08061626657112	27.84341176681093	25.546268216395923	21.529703750222023
32	26.04382190711313	28.062621677211112	25.40565616556785	20.487900250107902
33	24.41307997561328	28.739628115602134	25.84743636126387	20.99985554752072
34	18.397267011461498	29.167443116926645	28.26236759795602	24.17292227365584
35	18.675654433010926	28.666136280222098	29.125242045185257	23.532967241581716
36	25.9428360953142	26.619318838916286	26.588508128834153	20.849336936935366
37	19.143357520759043	30.98706430230107	28.34253654189493	21.52704163504496
38	19.731510409961373	30.995443418923973	25.517159514377823	23.755886656736834
39	19.185995004824537	29.179357173374832	27.53696303282126	24.097684788979368
40	22.138368018657502	26.9654374531675	27.07013276972136	23.826061758453633
41	17.777168740134353	25.14878387159702	27.984940283519634	29.089107104748997
42	21.09246224269675	26.577030484710072	26.27529500382079	26.055212268772387
43	21.14836666141517	26.46338871551197	27.652263168850983	24.735981454221875
44	20.288503459222287	29.377750215915	27.347429160460518	22.986317164402195
45	18.41847665041322	32.6870648696371	24.888682126427778	24.005776353521913
46	21.825242176108954	29.574528532855954	26.531032625748942	22.069196665286146
47	20.32865339304036	27.82992662599595	27.928337605082632	23.91308237588106
48	21.1704054837827	26.105836098369085	28.661423027121717	24.0623353907265
49	21.359459302586924	24.007041949261833	30.071864017411105	24.561634730740142
50	18.455353491800466	29.335810991568078	28.781567339949056	23.427268176682404
51	18.24085683450066	30.63256657142695	25.96640236081611	25.160174233256278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1397.0
1	1999.5
2	2602.0
3	8727.5
4	14853.0
5	10991.5
6	7130.0
7	6985.5
8	6841.0
9	7751.5
10	8662.0
11	8867.0
12	9072.0
13	9128.0
14	9184.0
15	8776.0
16	8368.0
17	8371.5
18	8375.0
19	8155.5
20	7936.0
21	8735.5
22	9535.0
23	10552.0
24	11569.0
25	12102.0
26	16884.5
27	21134.0
28	25668.0
29	30202.0
30	32981.5
31	35761.0
32	44870.0
33	53979.0
34	58175.5
35	62372.0
36	64740.0
37	67108.0
38	81546.0
39	95984.0
40	118116.0
41	140248.0
42	165501.5
43	190755.0
44	200837.0
45	210919.0
46	216912.0
47	222905.0
48	224881.5
49	226858.0
50	207005.0
51	187152.0
52	164756.0
53	142360.0
54	129067.0
55	115774.0
56	107189.5
57	98605.0
58	91403.5
59	84202.0
60	72753.5
61	61305.0
62	52968.0
63	44631.0
64	38433.0
65	32235.0
66	25750.0
67	19265.0
68	15879.0
69	12493.0
70	10377.0
71	8261.0
72	6271.0
73	4281.0
74	3427.0
75	1646.5
76	720.0
77	678.0
78	636.0
79	456.0
80	276.0
81	208.5
82	141.0
83	98.0
84	55.0
85	43.5
86	32.0
87	21.5
88	11.0
89	9.5
90	8.0
91	5.5
92	3.0
93	3.5
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2291411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.83460586013259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.83766765465747	15.240448531419892
2	9.35111156612226	3.7095122453632445
3	3.3945973600704824	2.0199150207253385
4	1.661127035844601	1.3179120015835202
5	0.9887069634081863	0.9805306465184956
6	0.6560026021380162	0.7806931833977354
7	0.4966379346764499	0.6895432382648362
8	0.3796082803344288	0.6023504497340894
9	0.28019046224071054	0.5001720645881571
>10	2.9541733407434965	13.954533362369595
>50	1.7785268833929269	26.53720778239582
>100	1.212933424603055	31.588223289933303
>500	0.006034494300845154	0.8471077453235446
>1k	0.0024584976781220993	0.8119538156547199
>5k	2.2349978892019086E-4	0.4198966227277521
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9472	0.41336975339648807	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3583	0.15636653572842235	No Hit
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	2448	0.10683373694199774	Illumina PCR Primer Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3641232410946795E-5	0.0	0.0	0.11289986824711935	0.0
2	4.3641232410946795E-5	0.0	0.0	0.4391617217513576	0.0
3	4.3641232410946795E-5	0.0	0.0	0.6487705610211351	0.0
4	4.3641232410946795E-5	0.0	0.0	1.0668099262855943	0.0
5	4.3641232410946795E-5	0.0	0.0	1.8616476921861682	0.0
6	4.3641232410946795E-5	0.0	0.0	2.675382111720682	0.0
7	4.3641232410946795E-5	0.0	0.0	3.133222280943925	0.0
8	4.3641232410946795E-5	0.0	0.0	4.208978659873764	0.0
9	4.3641232410946795E-5	0.0	0.0	4.559897809690186	0.0
10	4.3641232410946795E-5	0.0	0.0	5.221149763180852	0.0
11	4.3641232410946795E-5	0.0	0.0	6.042390474690049	0.0
12	8.728246482189359E-5	0.0	0.0	6.661615921368973	0.0
13	8.728246482189359E-5	0.0	0.0	6.906879647518494	0.0
14	8.728246482189359E-5	0.0	0.0	7.008825566430466	0.0
15	8.728246482189359E-5	0.0	0.0	7.168988889378641	0.0
16	8.728246482189359E-5	0.0	0.0	7.51838059606068	0.0
17	8.728246482189359E-5	0.0	0.0	7.941438703052398	0.0
18	8.728246482189359E-5	0.0	0.0	8.482459061250907	0.0
19	8.728246482189359E-5	0.0	0.0	8.73793483578459	0.0
20	8.728246482189359E-5	0.0	0.0	9.008030423175938	0.0
21	8.728246482189359E-5	0.0	0.0	9.35615653411806	0.0
22	8.728246482189359E-5	0.0	0.0	9.737537264157325	0.0
23	8.728246482189359E-5	0.0	0.0	10.111498984686728	0.0
24	8.728246482189359E-5	0.0	0.0	10.372124424644902	0.0
25	8.728246482189359E-5	0.0	0.0	10.600717200013442	0.0
26	8.728246482189359E-5	0.0	0.0	10.826386012810447	0.0
27	8.728246482189359E-5	0.0	0.0	11.08325830678128	0.0
28	8.728246482189359E-5	0.0	0.0	11.321714000674694	0.0
29	8.728246482189359E-5	0.0	0.0	11.598661261554561	0.0
30	8.728246482189359E-5	0.0	0.0	11.93971749284611	0.0
31	1.3092369723284039E-4	0.0	0.0	12.211951500625597	0.0
32	1.3092369723284039E-4	0.0	0.0	12.472140528259661	0.0
33	1.3092369723284039E-4	0.0	0.0	12.72792179142022	0.0
34	1.3092369723284039E-4	0.0	0.0	13.002861555609186	0.0
35	1.3092369723284039E-4	0.0	0.0	13.332963837565588	0.0
36	1.3092369723284039E-4	0.0	0.0	13.604586868091321	0.0
37	1.3092369723284039E-4	0.0	0.0	13.884938145099243	0.0
38	1.3092369723284039E-4	0.0	0.0	14.221761176846929	0.0
39	1.3092369723284039E-4	0.0	0.0	14.766447398567957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	35	1.2126293E-7	45.000004	44
CGGCCTA	20	7.0347544E-4	45.000004	44
TCCGAAC	60	0.0	45.000004	36
TATCGAT	20	7.0347544E-4	45.000004	1
AGTAACG	55	1.8189894E-12	45.0	1
CGACGGT	495	0.0	42.272728	28
CGGGTAT	195	0.0	41.538464	6
CGTTTTT	4785	0.0	41.52038	1
TCACGAC	520	0.0	40.67308	25
ATTAACG	155	0.0	40.64516	1
CGTTAGG	355	0.0	40.56338	2
TGCGTAG	180	0.0	40.0	1
GTAGGGC	900	0.0	39.5	4
TTAACGG	285	0.0	39.473686	2
ACGGTCT	550	0.0	38.863636	30
CACGACG	540	0.0	38.75	26
GGTACGA	35	6.251006E-6	38.57143	36
CGTAAGG	420	0.0	38.57143	2
TAGGGAC	2130	0.0	38.55634	5
CTCACGA	550	0.0	38.454544	24
>>END_MODULE