FastQCFastQC Report
Thu 26 May 2016
SRR1547892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547892_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3266155
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145410.44520238629213865No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44270.1355416384096897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72350.041.796821
ATCGTAG1200.041.2499961
CACGACG3950.041.0126626
TACGGGA9300.039.9193574
TAATGCG1300.039.8076931
TAACGGG6950.039.172663
ACGTTAG1100.038.8636361
CGGTCTA4100.038.41463531
TAAGGGA33650.038.112934
ATAGGGT18050.038.019394
TAGGGAT43350.037.8373685
AGGGATT46350.037.4757276
CTCACGA4650.037.25806424
TAGGGTA22250.037.213485
TTACGGG8900.037.162923
TTAGGGA36350.037.1389274
ACGGGAC7600.037.006585
GGCGATA8900.036.9101148
GCGCGAC5150.036.6990289
ACGGTCT4300.036.62790730
Produced by FastQC (version 0.11.5)