##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547892_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3266155
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.484255646164986	31.0	31.0	33.0	30.0	34.0
2	31.903788399509516	31.0	31.0	34.0	30.0	34.0
3	31.99661467382901	33.0	31.0	34.0	30.0	34.0
4	35.76592323389429	37.0	35.0	37.0	35.0	37.0
5	35.67835941650044	37.0	35.0	37.0	33.0	37.0
6	35.71991745645874	37.0	35.0	37.0	33.0	37.0
7	36.02891810094744	37.0	35.0	37.0	35.0	37.0
8	36.05336427695563	37.0	35.0	37.0	35.0	37.0
9	37.793620939606356	39.0	38.0	39.0	35.0	39.0
10	37.23287290407222	39.0	37.0	39.0	34.0	39.0
11	37.05137539400304	39.0	37.0	39.0	33.0	39.0
12	36.52562722834648	39.0	35.0	39.0	33.0	39.0
13	36.336497196244515	39.0	35.0	39.0	32.0	39.0
14	37.30284906870617	40.0	35.0	41.0	32.0	41.0
15	37.51020573120382	40.0	35.0	41.0	32.0	41.0
16	37.61924679018601	40.0	35.0	41.0	33.0	41.0
17	37.5607171123232	39.0	35.0	41.0	33.0	41.0
18	37.470765778109126	39.0	36.0	41.0	33.0	41.0
19	37.40903906887456	39.0	35.0	41.0	32.0	41.0
20	37.27088977712325	39.0	35.0	41.0	32.0	41.0
21	37.18006095852769	39.0	35.0	41.0	32.0	41.0
22	37.092211790316135	39.0	35.0	41.0	32.0	41.0
23	36.99880379222664	39.0	35.0	41.0	32.0	41.0
24	37.044756602182076	39.0	35.0	41.0	32.0	41.0
25	37.017929032761764	39.0	35.0	41.0	32.0	41.0
26	36.9299393323342	39.0	35.0	41.0	32.0	41.0
27	36.85176698595137	39.0	35.0	41.0	31.0	41.0
28	36.8070223244151	39.0	35.0	41.0	31.0	41.0
29	36.806177906437384	38.0	35.0	41.0	31.0	41.0
30	36.7176517954598	38.0	35.0	41.0	31.0	41.0
31	36.57018849381	38.0	35.0	41.0	31.0	41.0
32	36.46996697952179	38.0	35.0	41.0	31.0	41.0
33	36.38938170417509	38.0	35.0	41.0	30.0	41.0
34	36.25825932939496	38.0	35.0	41.0	30.0	41.0
35	36.197146491822956	38.0	35.0	41.0	30.0	41.0
36	36.084285038523895	38.0	35.0	41.0	30.0	41.0
37	35.99316811357697	38.0	35.0	41.0	30.0	41.0
38	35.87625541347548	38.0	35.0	40.0	29.0	41.0
39	35.779466681771076	38.0	35.0	40.0	29.0	41.0
40	35.6074298372245	38.0	35.0	40.0	28.0	41.0
41	35.544618060073695	38.0	35.0	40.0	28.0	41.0
42	35.47704961950673	38.0	34.0	40.0	27.0	41.0
43	35.42237585172779	38.0	34.0	40.0	27.0	41.0
44	35.262198823999476	38.0	34.0	40.0	27.0	41.0
45	35.14246935616956	38.0	34.0	40.0	27.0	41.0
46	35.1150747591587	37.0	34.0	40.0	27.0	41.0
47	35.04040745157533	37.0	34.0	40.0	26.0	41.0
48	34.92645970567839	37.0	34.0	40.0	26.0	41.0
49	34.79275539587068	37.0	34.0	40.0	26.0	41.0
50	34.72075820039159	37.0	34.0	40.0	26.0	41.0
51	34.3780950383555	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	13.0
12	10.0
13	18.0
14	37.0
15	90.0
16	240.0
17	531.0
18	1083.0
19	2069.0
20	3764.0
21	6210.0
22	9462.0
23	14354.0
24	21144.0
25	30207.0
26	39686.0
27	46995.0
28	52676.0
29	61444.0
30	73315.0
31	89630.0
32	112308.0
33	147688.0
34	253227.0
35	369599.0
36	231247.0
37	306879.0
38	492791.0
39	899311.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.221952111886914	23.985328314179824	29.410882214714245	12.381837359219022
2	30.670069240437147	25.506046100077924	30.303766967581147	13.520117691903783
3	29.421628795938954	25.419859130996535	30.955940547830707	14.202571525233799
4	26.267675600208808	27.912361783197674	30.633206323643552	15.186756292949969
5	23.439824503123706	32.3476381249512	29.245397110669884	14.967140261255206
6	21.81966256959636	40.390918373439106	26.851603797125367	10.93781525983917
7	88.51254762863367	3.711673205956239	5.935205157134306	1.840574008275786
8	89.04017108802246	2.8088072978777796	5.687880703763294	2.463140910336466
9	83.88493503829426	5.106248784886204	7.781167764542711	3.2276484122768205
10	40.369149657624945	29.75005778966399	19.33068087705574	10.55011167565532
11	35.73596476590976	21.599526048212653	25.806644203964602	16.857864981912986
12	32.7542936572208	21.411353717138347	27.802017969141087	18.032334656499767
13	24.90108399631983	29.05590824685295	28.596652638959263	17.446355117867952
14	18.968144500184465	32.80364832654911	30.2946124724638	17.933594700802626
15	17.74135030333833	26.19878725902475	39.6011518130646	16.45871062457232
16	21.799424705808512	23.82477255366019	36.441993720444984	17.933809020086308
17	22.088572036538377	23.13264373552388	28.10316717975724	26.675617048180506
18	23.779306248478715	25.148316598569266	31.790438604414057	19.281938548537962
19	25.981069483842624	27.05428860540911	26.69680404022467	20.267837870523596
20	27.07663904499327	26.29422057434506	26.89397778121369	19.735162599447975
21	24.442226409952987	26.39133782689431	30.10588903466002	19.060546728492678
22	23.24779442494309	22.924968349634355	29.87543457061897	23.951802654803583
23	20.796808479695546	27.403904591178314	28.971803236527354	22.82748369259879
24	20.870381228080113	26.432334044158956	33.277447028692755	19.41983769906817
25	21.01131146562242	26.605840812821192	30.82851242516047	21.554335296395916
26	19.85833495348506	30.190606385796144	27.45414715468188	22.496911506036916
27	19.102124669527317	29.717787428949332	30.72199574116966	20.45809216035369
28	18.02244535240979	27.63065439331569	33.54889770999846	20.798002544276066
29	19.028674389304857	26.187887592597413	32.9811965445608	21.802241473536927
30	21.228600602237186	26.800412105365485	31.545685982447253	20.42530130995008
31	25.25627228346481	26.51671460784929	27.50408354778019	20.722929560905715
32	25.6653771789765	26.44243766753262	27.959175238162302	19.933009915328576
33	25.01577543013115	25.7740982898852	27.799446137736883	21.410680142246772
34	19.688685931929133	26.396695808986408	30.98928250496379	22.92533575412067
35	20.519265007325128	25.959362002109515	30.637737645641433	22.883635344923924
36	26.20120600522633	24.483345095379736	29.0909647582555	20.224484141138433
37	20.76521169387246	27.984219977312776	30.041225845068592	21.20934248374618
38	21.77030790026805	28.05151623238946	27.226356373166617	22.951819494175872
39	20.484177878882047	26.61554641466801	29.7987082670602	23.101567439389743
40	22.65180311405919	25.339121995128828	29.52741067095713	22.481664219854842
41	19.982242116494778	23.878138055297438	30.07319003537799	26.066429792829794
42	22.03039965953851	25.420379620685484	28.780446733238318	23.768773986537685
43	21.609782756788945	25.149388194987683	29.516204834124526	23.724624214098842
44	20.975397677085137	27.409201339189355	28.92339769545536	22.69200328827015
45	20.287095989014606	29.114907283947026	26.74802634902508	23.849970378013293
46	22.453343457368067	27.73288469163282	28.408725244209172	21.40504660678994
47	21.39570840942944	26.415647757072154	29.860126050355845	22.328517783142562
48	21.692142595804548	25.581670190177746	30.221345894484493	22.504841319533213
49	21.895439744898816	23.67741273760737	31.068090767278346	23.35905675021547
50	20.70385514465786	26.924717289902038	29.482158685059346	22.889268880380754
51	19.645760841111336	28.30269843286678	27.786954385202172	24.26458634081971
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1954.0
1	3213.0
2	4472.0
3	14219.0
4	23966.0
5	17550.5
6	11135.0
7	10498.5
8	9862.0
9	10519.0
10	11176.0
11	11505.5
12	11835.0
13	11791.5
14	11748.0
15	11515.5
16	11283.0
17	10941.5
18	10600.0
19	10853.0
20	11106.0
21	12450.5
22	13795.0
23	15041.5
24	16288.0
25	19404.5
26	26910.0
27	31299.0
28	37782.5
29	44266.0
30	51996.0
31	59726.0
32	69643.0
33	79560.0
34	88057.5
35	96555.0
36	106840.5
37	117126.0
38	126382.0
39	135638.0
40	164362.0
41	193086.0
42	218964.5
43	244843.0
44	252441.5
45	260040.0
46	269338.5
47	278637.0
48	287325.5
49	296014.0
50	279294.0
51	262574.0
52	239819.5
53	217065.0
54	200348.5
55	183632.0
56	170012.0
57	156392.0
58	142876.0
59	129360.0
60	114991.0
61	100622.0
62	86152.0
63	71682.0
64	62670.5
65	53659.0
66	44050.0
67	34441.0
68	27161.0
69	19881.0
70	15813.5
71	11746.0
72	9757.0
73	7768.0
74	5924.0
75	3355.0
76	2630.0
77	1901.0
78	1172.0
79	780.5
80	389.0
81	324.5
82	260.0
83	197.0
84	134.0
85	92.5
86	51.0
87	43.0
88	35.0
89	28.0
90	21.0
91	19.0
92	17.0
93	13.0
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3266155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.70682114681483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.14592670278924	14.244482318875495
2	9.492725910517898	3.551574516075865
3	3.3951883222706885	1.905395421134162
4	1.6709851395986821	1.250352805818318
5	0.9857674551567368	0.922028773798394
6	0.6764466425291661	0.7592499814293896
7	0.473787674510897	0.6204142902048467
8	0.3516321325969203	0.5262335531170931
9	0.2944837125563601	0.4957968727297671
>10	3.2370437235826914	14.583884255113825
>50	1.9991431139506004	27.97082878277191
>100	1.2675288062489822	31.07304253571427
>500	0.00617150993888086	0.7386885137135814
>1k	0.0030023561864825805	0.9043115022576249
>5k	0.0	0.0
>10k+	1.6679756591569893E-4	0.453715877245467
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14541	0.44520238629213865	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4427	0.1355416384096897	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08624820316243412	0.0
2	0.0	0.0	0.0	0.2813399853956717	0.0
3	0.0	0.0	0.0	0.42058628570903706	0.0
4	0.0	0.0	0.0	0.6702682512005707	0.0
5	0.0	0.0	0.0	1.184328361636236	0.0
6	0.0	0.0	0.0	1.7335368345960311	0.0
7	0.0	0.0	0.0	2.0457387968421585	0.0
8	0.0	0.0	0.0	2.76925008151787	0.0
9	0.0	0.0	0.0	3.046089361956184	0.0
10	0.0	0.0	0.0	3.594746728186507	0.0
11	0.0	0.0	0.0	4.2120781163171985	0.0
12	0.0	0.0	0.0	4.70048114679187	0.0
13	0.0	0.0	0.0	4.895695397187213	0.0
14	0.0	0.0	0.0	4.983994942064905	0.0
15	0.0	0.0	0.0	5.116842280908285	0.0
16	0.0	0.0	0.0	5.419644811712855	0.0
17	0.0	0.0	0.0	5.756309789339452	0.0
18	0.0	0.0	0.0	6.190245104717933	0.0
19	0.0	0.0	0.0	6.4071056027653315	0.0
20	0.0	0.0	0.0	6.652929821150558	0.0
21	0.0	0.0	0.0	6.96353969728932	0.0
22	0.0	0.0	0.0	7.305225869562222	0.0
23	0.0	0.0	0.0	7.636471631015675	0.0
24	0.0	0.0	0.0	7.887990618938783	0.0
25	0.0	0.0	0.0	8.109688609389329	0.0
26	0.0	0.0	0.0	8.32064001861516	0.0
27	0.0	0.0	0.0	8.563984256717761	0.0
28	0.0	0.0	0.0	8.802062363849847	0.0
29	0.0	0.0	0.0	9.066379274712927	0.0
30	0.0	0.0	0.0	9.390828053169553	0.0
31	0.0	0.0	0.0	9.66754486544576	0.0
32	0.0	0.0	0.0	9.927514156554114	0.0
33	0.0	0.0	0.0	10.185523957068785	0.0
34	0.0	0.0	0.0	10.469190837544453	0.0
35	0.0	0.0	0.0	10.797497363107384	0.0
36	0.0	0.0	0.0	11.069897172669393	0.0
37	0.0	0.0	0.0	11.349369518592964	0.0
38	0.0	0.0	0.0	11.673910148171167	0.0
39	0.0	0.0	0.0	12.215280658756244	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7235	0.0	41.79682	1
ATCGTAG	120	0.0	41.249996	1
CACGACG	395	0.0	41.01266	26
TACGGGA	930	0.0	39.919357	4
TAATGCG	130	0.0	39.807693	1
TAACGGG	695	0.0	39.17266	3
ACGTTAG	110	0.0	38.863636	1
CGGTCTA	410	0.0	38.414635	31
TAAGGGA	3365	0.0	38.11293	4
ATAGGGT	1805	0.0	38.01939	4
TAGGGAT	4335	0.0	37.837368	5
AGGGATT	4635	0.0	37.475727	6
CTCACGA	465	0.0	37.258064	24
TAGGGTA	2225	0.0	37.21348	5
TTACGGG	890	0.0	37.16292	3
TTAGGGA	3635	0.0	37.138927	4
ACGGGAC	760	0.0	37.00658	5
GGCGATA	890	0.0	36.910114	8
GCGCGAC	515	0.0	36.699028	9
ACGGTCT	430	0.0	36.627907	30
>>END_MODULE