FastQCFastQC Report
Thu 26 May 2016
SRR1547891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547891_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2703293
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110770.4097595044266382No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42250.15629086451228186No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG207.034982E-445.034
CGTACCG253.8922117E-544.99999633
TTTCGCG1450.043.4482731
CGTAAGG4150.042.8313262
GCGCGAC4650.040.645169
TAATGCG1550.040.645161
CGACGGT3500.040.528
CCGCTCG1800.040.019
CGGTCTA3550.039.92957731
CGTTTTT60200.039.617941
CGGGATA5950.039.3277326
TAACGCG1150.039.1304361
CGAACGG1500.039.02
TACGCGG1850.038.9189222
TTAGGGA38500.038.7467544
GGGCGAT37050.038.6842167
CGCGCGA356.2514246E-638.57142629
TACGCAC356.2514246E-638.57142631
CACGACG3800.038.48684326
CGTGCGG2400.038.4375042
Produced by FastQC (version 0.11.5)